Octanediol treatment of glutaraldehyde fixed bovine pericardium: evidence of anticalcification efficacy in the subcutaneous rat model☆

Pettenazzo, Elena; Valente, Marialuisa; Thiene, Gaetano

doi:10.1016/j.ejcts.2008.05.012

Cited by 41 publications

(41 citation statements)

References 20 publications

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“…After centrifugation (15 minutes, 3000 rpm), the fluid was removed and diluted, and the calcium content was determined using a fast sequential atomic absorption spectrometer (Varian AA240FS, Varian Inc, Palo Alto, CA). 29 …”

Section: Cytotoxicity Assay In Vitromentioning

confidence: 99%

Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds

Tao¹,

Hu²,

Wu³

et al. 2012

IJN

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Biocompatibility and biomechanical stability are two of the main obstacles limiting the effectiveness of vascular scaffolds. To improve the biomechanical stability and biocompatibility of these scaffolds, we created a heparin-nanomodified acellular bovine jugular vein scaffold by alternating linkage of heparin and dihydroxy-iron via self-assembly. Features of the scaffold were evaluated in vitro and in vivo. Heparin was firmly linked to and formed nanoscale coatings around the fibers of the scaffold, and the amount of heparin linked was about 808 ± 86 µg/cm 2 (101 ± 11 USP/cm 2 ) per assembly cycle. The scaffolds showed significantly strengthened biomechanical stability with sustained release of heparin for several weeks in vitro. Importantly, the modified scaffolds showed significantly reduced platelet adhesion, stimulated proliferation of endothelial cells in vitro, and reduced calcification in a subcutaneous implantation rat model in vivo. Heparin nanomodification improves the biocompatibility and biomechanical stability of vascular scaffolds.

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Section: Cytotoxicity Assay In Vitromentioning

confidence: 99%

Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds

Tao¹,

Hu²,

Wu³

et al. 2012

IJN

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“…Most studies used the implant model in TCSC of rats, which limits its interpretation because it does not assess the influence of bloodstream and tissue stress effects on calcification [12,13,15,16,21]. Our research group evaluated the effect of SDS decellularization in GDA-fixed bovine pericardium and implanted in the TCSC of rats for 90 days, with a reduction of 98% in the final amount of calcium (65.91 µg/mg tissue versus 1.24 µg/mg tissue) (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

“…Several treatments were tested in order to eliminate or retard the calcification, but no method is quite effective yet [12][13][14][15][16]. The decellularization has been proposed as an alternative during the biological tissue processing of cardiovascular prostheses.…”

Section: Introductionmentioning

confidence: 99%

Descelularização como método anticalcificante em próteses valvares de pericárdio bovino sem suporte

Collatusso¹,

Roderjan²,

Vieira³

et al. 2011

Rev Bras Cir Cardiovasc

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Descelularização como método anticalcificante em próteses valvares de pericárdio bovino sem suporte: estudo em ovinosDecellularization as an anticalcification method in stentless bovine pericardium valve prosthesis: a study in sheep Work performed at the Cardiac Surgery Department at Santa Casa Hospital, PUCPR, Curitiba, Parana, Brazil. Methods:The valved tubs were implanted in pulmonary artery position in sheep by 180 days. The animals were divided in two groups of eight animals: control groupglutaraldehyde-preserved bovine pericardium and the study group -decellularized bovine pericardium with 0.1% SDS and glutaraldehyde-preserved. After explantation the tubs were analized by x-ray macroscopy, hematoxilin-eosin, alizarin-red and Russel-Movatz pentacromic histology. The calcium content was measured by flame atomic absorption spectrometry.Results: There was no early mortality, but two animals in each group died during the study. All cusps in the control group were severely calcified and in some points in the conduits, while the decellularized group did not show macroscopic calcification. Data were proved by x-ray and histologycal exams. The matrix was preserved in histologycal analysis in decellularized group, without gross calcification.

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“…This study used the subcutaneous implantation of specimens. The subcutaneous model has emerged as a technically convenient and economically advantageous vehicle for investigating host and implant determinants and mechanisms of mineralization, as well as for screening potential strategies for inhibition of calcification [11] [13] [14]. The rat subcutaneous implant is a favorable low cost model with easy availability.…”

Section: Commentsmentioning

confidence: 99%

Does the Animal Origin Influence the Calcification of Xenograft Tissue Heart Valve Substitutes? Comparison between Bovine and Camel Pericardium in a Subcutaneous Rat Model

Harmoodi¹,

Shafy²,

Guichardant³

et al. 2015

IJCM

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Objective: To validate the hypothesis that camel pericardium could be more protected than bovine pericardium against calcification process according to the huge difference in their respective lifestyle and lifetime. Methods: Glutaraldehyde (GA) fixed bovine and camel pericardium samples (BP and CP respectively) were both implanted in 30 New Zealand white rats (2 BP and 2 CP matched specimens in each animal) and explanted after 60 days. Unimplanted GA-fixed samples of both species served as control. Matched implanted samples and unimplanted samples were randomly submitted to elemental analysis by spectroscopy, phospholipid extraction, macroscopic and X-ray examination and histology. Results: At 60 days, calcium and phosphorus content were respectively 9.54% ± 3.1% and 4.79% ± 1.4% of tissue dry weight in BP, and 12.52% ± 2.7% and 6.14% ± 1.3% of tissue dry weight in CP (ns). In X-ray analysis, the calcification score was 1.28 ± 0.45 and 2.14 ± * Corresponding author. F. Al Harmoodi et al. 7010.98 in BP and CP samples respectively without significant difference (p < 0.08). In histology, calcifications were lower in BP than in CP: 1.37 ± 0.85 vs 2.28 ± 0.83 (ns); collagen fibers were better conserved in BP than in CP: 2.4 ± 0.48 vs 1.87 ± 0.78 (ns), and less disoriented: 25% vs 62% (ns). In unimplanted samples, there was a higher but not significant rate of extracted lipids in CP: 5.7 ± 1.8 vs 9.5 ± 3.8 nanomoles in PS fraction and 11.3 ± 3.7 vs 19 ± 7.7 nanomoles in total fatty acids, in BP and CP samples respectively. All results were in conjunction and demonstrated a higher but not significant rate of mineralization in camel pericardium after implantation, which could be related to a higher but not significant basic rate of phospholipid and fatty acids. Conclusion: This experiment study in a subcutaneous rat model has failed to valid our hypothesis. Because the differences observed between bovine and camel pericardium did not reach the significance, at the best, there is no difference between both species and at the worst, camel pericardium has a higher rate of the phosphatidylserine fraction of phospholipid, and is more sensitive and prompt to calcification.

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Octanediol treatment of glutaraldehyde fixed bovine pericardium: evidence of anticalcification efficacy in the subcutaneous rat model☆

Abstract: Treatment with octanediol strongly prevents calcification of glutaraldehyde fixed bovine pericardium in rat subdermal model, even in the long-term. Evidence of octanediol efficacy may entail important implications for new generation bioprosthetic valves.

Cited by 41 publications

References 20 publications

Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds

Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds

Descelularização como método anticalcificante em próteses valvares de pericárdio bovino sem suporte

Does the Animal Origin Influence the Calcification of Xenograft Tissue Heart Valve Substitutes? Comparison between Bovine and Camel Pericardium in a Subcutaneous Rat Model

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