Octreotide inhibits growth factor–induced and basal proliferation of lens epithelial cells in vitro

Baldysiak-Figiel, Alicja; Jong‐Hesse, Yvonne de; Lang, Gerhard K.; Lang, Gabriele E.

doi:10.1016/j.jcrs.2004.08.056

Cited by 6 publications

(8 citation statements)

References 29 publications

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“…In a key paper, Baldysiak‐Figiel et al . () demonstrated that octreotide inhibited growth factor‐induced proliferation of lens epithelial cells in vitro as measured by thymidine incorporation. However, a caveat must be added that systemic injection of long‐acting somatostatin analogues does not unequivocally provide evidence for a direct site of action of these compounds in the rat lens in experimental diabetes.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, attention has been drawn to the role of growth factors such as fibroblast growth factors and insulin-like growth factor 1 and to the effect of a somatostatin analogue, octreotide, on lens epithelial cell proliferation (Baldysiak-Figiel et al 2005;Kampmeier et al 2006). IGF-1 has been shown to regulate lens cell differentiation and proliferation and specifically binds to bovine lens epithelial cells (Palmade et al 1994;Klok et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, attention has been drawn to the role of growth factors such as fibroblast growth factors and insulin‐like growth factor 1 and to the effect of a somatostatin analogue, octreotide, on lens epithelial cell proliferation (Baldysiak‐Figiel et al . ; Kampmeier et al . ).…”

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confidence: 99%

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Effects of long‐acting somatostatin analogues on redox systems in rat lens in experimental diabetes

Kunjara

Greenbaum

Sochor

et al. 2014

Int J Experimental Path

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The effects of long-acting somatostatin analogues, angiopeptin (AGP) and Sandostatin (SMS), on the early decline in the lens content of glutathione (GSH), ATP and NADPH and increase in sorbitol were studied in STZ diabetic rats, and comparison was made with the effect of insulin. Three factors prompted this study: (i) the known increase in IGF-1 in ocular tissue in diabetes and antagonistic effect of somatostatins, (ii) the known effect of IGF-1 in increasing lens aldose reductase and (iii) the lack of effect of somatostatins on diabetic hyperglycaemia, the latter enabling a differentiation to be made between effects of hyperglycaemia per se and site(s) of IGF-1/somatostatins. All four metabolites studied showed a significant restoration towards the normal control level after 7 days of treatment with AGP and SMS, and AGP was more effective on levels of GSH and ATP. A significant correlation was found between GSH and ATP across all groups at 7 days treatment. The redox state changes in diabetes include both NADP+/NADPH and NAD+/NADH in the conversion of glucose to sorbitol and via sorbitol dehydrogenase to fructose with a linked decrease in ATP formation via NAD+/NADH regulation of the glycolytic pathway. The interlinked network of change includes the requirement for ATP in the synthesis of GSH. The present study points to possible loci of action of somatostatins in improving metabolic parameters in the diabetic rat lens via effects on aldose reductase and/or glucose transport at GLUT 3.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of long‐acting somatostatin analogues on redox systems in rat lens in experimental diabetes

Kunjara

Greenbaum

Sochor

et al. 2014

Int J Experimental Path

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“…It has been suggested that OCT has an inhibitory effect on lens epithelial cell (LEC) proliferation induced by basal and bFGF and IGF-1. Such mechanisms commonly include the massive overproduction of cytokines, including transforming growth factor (TGF-β), leading to the myofibroblastic conversion of LECs (Baldysiak-Figiel et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

The effect of octreotide, an analog of somatostatin, on bleomycin-induced interstitial pulmonary fibrosis in rats

Tuğ

Kara

Karaoğlu

et al. 2012

Drug and Chemical Toxicology

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In this study, octreotide (OCT), a synthetic somatostatin analog, was tested for its beneficial effects in the prevention of interstitial pulmonary fibrosis (IPF) induced by bleomycin (BLM) in rats by histological examination and by evaluating tissue OH-proline levels. Thirty male Wistar rats were divided randomly into three groups: group I: intratracheal (i.t.) BLM (7.5 mg/kg, single dose) + saline solution [0.9% NaCl, subcutaneously (s.c.), once-daily for 7 days]; group II: i.t. BLM (7.5 mg/kg, single dose) + OCT acetate (82.5 µg/kg, s.c., once-daily for 7 days); and the control group. At the end of the 7 days, lung tissues were excised and examined by histopathological methods. Levels of tissue hydroxyproline (OH-proline) were determined. BLM administration resulted in prominent histopathologic findings, such as diffuse alveolar damage and interstitial pulmonary fibrosis, as well as a significant increase in OH-proline level, as compared to controls. OCT application explicitly attenuated the histopathologic changes to a significant extent. OCT decreased paranchymal fibrosis and structural deformities in BLM-induced lung fibrosis. These results suggest that OCT administration to rats with BLM-induced IPF has a protective effect. Further studies are necessary to reveal the molecular mechanism(s) of OCT-induced protective effect.

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“…Since radiationand somatostatin analogs induce apoptosis in tumor cells throughdifferent signaling pathways, combination of the two modalitiesmay lead to synergistic effect on apoptotic cell death. Furthermore, it has reported that somatostatin analogs inhibit the expression and secretion of the growth factors, such as insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF), in tumor cells (Baldysiak-Figiel et al 2005, Kanashiro et al 2005. Since ionizing radiation activates and/or induces the overexpression of VEGF, bFGF and other growth factors in tumor cells (Kim et al 2006), combined use of lanreotide and ionizing radiation could inhibit the pro-survival autocrine and paracrine effects of these radiation-induced growth factors in targeted tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Lanreotide promotes apoptosis and is not radioprotective in GH3 cells

Ning

Knox

Harsh

et al. 2009

Endocrine-Related Cancer

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Somatostatin analogs are a mainstay of medical therapy in patients with GH producing human pituitary tumors, and it has been suggested that somatostatin analogs may be radioprotective. We utilized GH secreting rat GH3 cells to investigate whether a somatostatin analog may limit the effects of radiation on proliferation and apoptosis in vitro and on tumor growth in vivo. Treatment with lanreotide alone at doses of either 100 or 1000 nM for 48 h reduced clonogenic survival by 5-10%. Radiation alone produced a dose-dependent survival curve with a SF2 of 48-55%, and lanreotide had no effect on this curve. The addition of lanreotide resulted in a 23% increase in the proportion of apoptotic sub-G1 cells following irradiation (P!0.01). In a mouse GH3 tumor xenograft model, lanreotide 10 mg/kg moderately inhibited the growth of GH3 tumors, with a 4! tumor growth delay (TGD) time that ranged from 4.5 to 8.3 days. Fractionated local tumor radiation alone significantly inhibited tumor growth and produced a TGD of 35.1G5.7 days for 250 cGy fractions. The combination of lanreotide, either antecedent to or concurrent, with radiation of 250, 200 or 150 cGy/fraction for 5 days inhibited tumor growth and produced the TGD times that were similar to radiation alone (PO0.05). Pretreatment with lanreotide had the most significant radiosensitizing effect. These studies demonstrate that the somatostatin analog lanreotide is not radioprotective in GH3 cells, and further studies are necessary to determine the impact of lanreotide on apoptosis.

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Octreotide inhibits growth factor–induced and basal proliferation of lens epithelial cells in vitro

Cited by 6 publications

References 29 publications

Effects of long‐acting somatostatin analogues on redox systems in rat lens in experimental diabetes

Effects of long‐acting somatostatin analogues on redox systems in rat lens in experimental diabetes

The effect of octreotide, an analog of somatostatin, on bleomycin-induced interstitial pulmonary fibrosis in rats

Lanreotide promotes apoptosis and is not radioprotective in GH3 cells

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