Octreotide reduces ischaemia‐reperfusion injury in the retina

Abstract

ABSTRACT.Purpose: To investigate the role of octreotide on retinal lipid peroxidation and histopathological changes during ischaemia/reperfusion (I/R). Methods: Three groups of seven pigmented guinea pigs each were formed. These represented a control group, an ischaemia group and an ischaemia/octreotide group. One eye of each animal was selected for histopathological evaluation and the other for biochemical assay. Bilateral pressure-induced retinal ischaemia was instigated for 90 min and was followed by 24 hours of reperfusion. Animals in the ischaemia/octreotide and ischaemia groups received either 10 mg/kg of octreotide or saline, repeated five times at 6-hourly intervals, with the first dose administered 15 min prior to the ischaemic insult. Retinal malondialdehyde (MDA) levels and the thickness of the retinal layers were measured. These were compared with equivalent measurements of the control group. Results: The mean MDA level increased in the ischaemia group (p ∞0.01) but not in the octreotide group (p Ͼ0.05). Significant increases in the thickness of the overall retina (p ∞0.01), inner retina (p ∞0.05) ganglion cell layer (p ∞0.01) inner plexiform layer (p ∞0.01) and inner nuclear layer (p ∞0.01) were observed in the ischaemia group. No significant difference in thickness was found in any of the layers in the ischaemia/octreotide group. Conclusion: Octreotide reduces the increases in retinal MDA levels and retinal thickness observed during I/R.

Somatostatin and Neuroprotection in Retina

“…It was also efficient in reversing retinal cell death as observed with the TUNEL assay [103]. Comparable results were also observed in an earlier study where octreotide reduced the increase of lipid peroxidation levels observed subsequent to ischemia/reperfusion retinal injury [106]. In agreement with these data, the increased presence of functional sst 2 receptors, in retinas of sst 1 k.o.…”

Occludin downregulation in high glucose is regulated by SSTR2 via the VEGF/NRP1/Akt signaling pathway in RF/6A cells

“…It has been indicated that OCT was associated with the integrity of blood vessel endothelium, as confirmed by previous studies (14)(15)(16). Damage of endothelial cells is often observed in the early stage of DR (3,17,18).…”

Anti‐inflammatory and retinal protective effects of capsaicin on ischaemia‐induced injuries through the release of endogenous somatostatin

“…This will lead to an increase in the whole retinal and sub‐layer thickness. Along with the edema and inflammatory reaction, the neuronal injury will be aggravated (decrease in the number of cells) and lead to the reduction of retinal thickness at 7 days after retina I/R . For the retinal I/R injury, our results corresponded to those of previous reports.…”