Octreotide regulates CC but not CXC LPS-induced chemokine secretion in rat Kupffer cells

Expression, pharmacology, and functional role of somatostatin receptor subtypes 1 and 2 in human macrophages

“…Accordingly, our study showed that SRIF-14 decreased IL-8 secretion significantly in LPS-activated macrophages, although its effect appeared marginal. This is in contrast to previous studies, which failed to detect an effect of SMS 201-995 on the secretion of IL-8 by rat macrophage Kupffer cells activated with LPS [10]. We also found that SRIF-14 (and its synthetic analogs) did not affect IL-8 mRNA expression, suggesting the coupling of SRIF receptors to transduction pathways, which may not interact with nuclear regulatory factors of the IL-8 gene but rather with biochemical events triggering exocytosis of IL-8.…”

“…Cells of the monocyte lineage, i.e., macrophages, are known to be important components of the human immune circuitry and are involved in the modulation of immunological and inflammatory responses. Although the inhibitory role of SRIF-14 in cells of the monocyte lineage has been studied extensively in rodents [7][8][9][10][11][12][13][14], little is known about whether these findings can be extended to human monocytes. In this article, we report for the first time the expression and functional effects of distinct SRIF receptors in human macrophages differentiated from PBMC-derived monocytes.…”

“…In line with its effect on cell viability, SRIF-14 had no effect on MCP-1 production. Recently, SMS 201-995 was found to inhibit the LPS-induced mRNA expression and secretion of MCP-1 by rat Kupffer cells, the resident macrophages of the liver [10]. MCP-1 is a potent mononuclear cell chemoattractant, which has a critical role in vascular and nonvascular diseases, and efforts are under way to develop potent and specific modulators of this and related chemokines.…”

“…In rodent cells of the monocyte lineage, the inhibitory role of SRIF-14 has been studied extensively [7][8][9][10][11][12][13][14]. However, in human monocytes, the role of SRIF-14 is less well-understood, and reported results have been controversial [5,15].…”

Immunosuppression using the mTOR inhibition mechanism affects replacement of rat liver with transplanted cells

“…Liver RNA was extracted with Trizol reagent (Life Technologies) from 2 to 3 DPPIV Ϫ rats each 6, 12, and 24 hours after transplantation of F344 cells with unmanipulated rats and rats subjected to laparotomy alone serving as controls. Polymerase chain reaction primers have been published previously for vascular endothelial growth factor, Tie-2, angiopoietin 1, angiopoietin 2, ␤-actin, 15 macrophage inflammatory protein 2, cytokine-induced neutrophil chemoattractant 1, tumor necrosis factor ␣, 16 monocyte chemotactic protein 1, 17 and interferon-inducible protein 10. 18 RNAs (1 g) were reverse-transcribed (Omniscript RT PCR kit, Qiagen) and amplified for 35 cycles in 50 L using 1ϫ polymerase chain reaction buffer, 10 U RT, 100 U RNase inhibitor, 1.25 g oligo-dT primer, and dNTPs (Platinum PCR kit, Invitrogen).…”