Ocular experimental leishmaniasis in C57BL/10 and BALB/c mice induced by Leishmania amazonensis infection

Calabrese, Kátia da Silva; Silva, L.S.; Hardoim, Daiana de Jesús; Souza, C. S. F.; Abreu-Silva, Ana Lúcia

doi:10.1016/j.exppara.2012.11.008

Cited by 3 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was possible to identify and characterize by ultrasonography the following pathologies: lens rupture, lens dislocation, retinal detachment and cataract. Calabrese et al [4] evaluated ocular changes in mice experimentally infected with Leishmania amazonensis and found that ocular lesions may be associated with the deposition of immunoglobulins in response to type III hypersensitivity stimulated by the presence of parasite antigens. This accumulation of immunocomplexes favors the appearance of ocular lesions.…”

Section: Discussionmentioning

confidence: 99%

B-Mode and Doppler Ocular Ultrasound Evaluation in Healthy and Positive Dogs for Visceral Canine Leishmaniasis

Silva¹,

Rodrigues²,

Pessoa³

et al. 2020

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background: The use of ultrasound examination in the evaluation of ophthalmopathies has been gaining more and more space within the ophthalmologic clinical routine. The hemodynamic study of ocular vascularization may anticipate future changes, aiding in the adequate establishment of therapeutic conduits. The objective of this study was to evaluate the structures of the ocular bulb and to perform the hemodynamic evaluation of the flow of the external ophthalmic artery of dogs with canine visceral leishmaniasis (CVL) correlating with healthy animals.Materials, Methods & Results: For this purpose, 100 animals were used, of these 70 positive for CVL and 30 healthy animals, submitted to B-mode and Doppler ultrasound examination. Two-dimensional evaluation included identification of ocular changes and biometry of the following segments: axial length (M1), anterior chamber depth (M2), lens thickness (M3), lens length (M4), glass chamber depth (M5), optical disc length (M6) and optic nerve length (M7). The Doppler velocimetric evaluation included the identification and hemodynamic evaluation of the external ophthalmic artery, being measured: systolic peak velocity (SPV), final diastolic velocity (FDV), resistivity index (RI) and pulsatility index (PI). Ophthalmopathies were frequent in animals with leishmaniasis in both right (91.42%) and left (29.14%) eyes, with identification of capsular cataract, lens dislocation, retinal detachment and lens rupture. No significant statistical difference (P > 0.05) was observed when comparing the biometric values between the right and left eyes of the animals with CVL, as well as for the measurements between healthy and CVL animals. Hemodynamic indexes of the flow of the external ophthalmic artery presented narrow limits for the right and left eyes of the positive animals, not statistically different from each other. However, a significant difference was observed when compared to hemodynamic evaluations of the flow of the ophthalmic artery between the right eyes of the group of normal animals and that of the carriers of leishmaniasis, with the latter presenting values superior to the first one. The B-mode and Doppler ultrasonographic evaluation enabled the morphological characterization of the ocular bulb, the identification of ophthalmopathies and the hemodynamic evaluation of the external ophthalmic artery of dogs with canine visceral leishmaniasis, establishing values that could be used in clinical ophthalmologic routine.Discussion: The presence of ocular changes diagnosed by B-mode ultrasonography were common in dogs with canine visceral leishmaniasis. Ocular lesions were observed in 80.5% of dogs with leishmaniasis, presenting a high frequency, corroborating with the results in the present research. In 22% of the animals, B-mode ultrasonographic lesions were identified in both eyes, 64 of these presented changes only in the right eye and 16 in the left eye. Ophthalmopathies can be uni or bilateral and cause more than one alteration in the same eye, resulting from both the immune-mediated mechanisms caused by the agent and direct parasitism. Although the literature reports that the highest frequency of lesions is bilateral, only 16 animals had alterations in both eyes. The origin of ophthalmopathies may be related to the species and tropism of the parasite, type and duration of the immune response developed by the host. The greater frequency of bilateral ophthalmopathies can have correlation with the systemic disease, in which in the initial stages of the disease only one eye is affected and the occurrence of bilateral manifestations is related to the chronic cases.

show abstract

Section: Discussionmentioning

confidence: 99%

B-Mode and Doppler Ocular Ultrasound Evaluation in Healthy and Positive Dogs for Visceral Canine Leishmaniasis

Silva¹,

Rodrigues²,

Pessoa³

et al. 2020

Acta Scientiae. Vet.

View full text Add to dashboard Cite

show abstract

“…On the other hand, BALB/c mice infected via the instillation route presented no lesions but an enhancement of intact MCs in the conjunctival region at 30 days p.i., and a discreet inflammatory infiltrate and degranulated MCs were observed in the conjunctival region at 60 days p.i. (32). …”

Section: Mcs In Leishmania Spp Infectionmentioning

confidence: 99%

The Roles of Mast Cells in Parasitic Protozoan Infections

Huang

2017

Front. Immunol.

View full text Add to dashboard Cite

Protozoan parasites such as Plasmodium spp., Leishmania spp., Trypanosoma spp., and Toxoplasma gondii are major causes of parasitic diseases in both humans and animals. The immune system plays a critical role against protozoa, but their immune mechanism remains poorly understood. This highlights the need to investigate the function of immune cells involved in the process of parasite infections and the responses of host immune system to parasite infections. Mast cells (MCs) are known to be central players in allergy and anaphylaxis, and it has been demonstrated that MCs have crucial roles in host defense against a number of different pathogens, including parasites. To date, there are many studies that have examined the interaction of helminth-derived antigens and MCs. As one of the major effector cells, MCs also play an important role in the immune response against some parasitic protozoa, but their role in protozoan infections is, however, less well characterized. Herein, we review the current knowledge about the roles of MCs and their mediators during infections involving highly pathogenic protozoa including Plasmodium spp., Leishmania spp., Trypanosoma spp., and T. gondii. We offer a general review of the data from patients and experimental animal models infected with the aforementioned protozoa, which correlate MCs and MC-derived mediators with exacerbated inflammation and disease progression as well as protection against the parasitic infections in different circumstances. This review updates our current understanding of the roles of MCs during parasitic protozoan infections, and the participation of MCs in parasitic protozoan infections could be of a potential therapeutic target.

show abstract

“…Literatürde göz ve çevresinin değerlendirildiği birkaç çalışmada tutulum oranı %2-5 olarak rapor edilmiştir 10,[13][14][15] . Durdu ve ark.…”

unclassified

Perioküler kutanöz layşmanyazis: Pitozis gelişmiş iki olgu sunumu

Turan¹,

Yeşilova²,

Başaran³

et al. 2014

TURKDERM

View full text Add to dashboard Cite

Türk derm-De ri Has ta lık la rı ve Fren gi Ar şi vi Der gi si, Ga le nos Ya yı ne vi ta ra f›n dan ba s›l m›fl t›r. Turk derm-Arc hi ves of the Tur kish Der ma to logy and Ve ne ro logy, pub lis hed by Ga le nos Pub lis hing. ÖzetKutanöz layşmanyazis (KL) insanlara tatarcık ısırığı ile bulaşan, Leishmania genusundan bazı kamçılı parazitlerin neden olduğu parazitik bir hastalıktır. Dünyanın pek çok bölgesinde olduğu gibi ülkemizde de önemli bir sağlık sorunudur. Hastalık sıklıkla çocukluk yaş grubunda görülmekte birlikte her yaş grubunu etkileyebilir. Lezyonlar sıklıkla yüz, boyun, el, kol ve bacak gibi vücudun dışa açık alanlarında yerleşim göstermektedir. Daha nadir olarak saçlı deri, gövde, göz kapağı ve penis gibi diğer bölgelerde de görülebilmektedir. Göz kapağı tutulumu muhtemelen kapağın hareketli olması ve dişi tatarcık sineğinin rahatça konamaması nedeniyle sık değildir. Burada klinik ve laboratuvar bulguları ışığında kutanöz layşmanyazis tanısı konulan iki olgu sunulmuş, göz kapağı yerleşimli lezyonların erken tedavi edilmediğinde kozmetik yakınmalarla birlikte göz kapağı fonksiyon bozukluğuna yol açabileceğine dikkat çekilmiştir. (Türk derm 2014; 48: 43-6) Anah tar Ke li me ler: Kutanöz layşmanyazis, pitozis, lagoftalmus Sum maryLeishmaniasis is a parasitic disease caused by flagellate protozoa of the genus Leishmania. It is transmitted to humans via the bite of sandflies. The disease is a major health problem in our country as in many regions in the world. Although the disease is more often seen in children, it may affect any age group. The lesions usually occur on the exposed areas of the body such as face, neck, hands, arms and lower legs. However, they may be seen in other areas of the body such as scalp, trunk, eyelids and, penis. Eyelid involvement is not common, probably due to the movement of the lids preventing the female phlebotomine sandfly from biting the skin. In this paper, we present two cases of cutaneous leishmaniasis diagnosed on the basis of clinical and laboratory findings and we aim the draw attention to the fact that if not treated early, palpebral lesions can lead to dysfunction of palpebrae along with cosmetic problems. (Turkderm 2014; 48: 43-6)

show abstract

Ocular experimental leishmaniasis in C57BL/10 and BALB/c mice induced by Leishmania amazonensis infection

Cited by 3 publications

References 41 publications

B-Mode and Doppler Ocular Ultrasound Evaluation in Healthy and Positive Dogs for Visceral Canine Leishmaniasis

B-Mode and Doppler Ocular Ultrasound Evaluation in Healthy and Positive Dogs for Visceral Canine Leishmaniasis

The Roles of Mast Cells in Parasitic Protozoan Infections

Perioküler kutanöz layşmanyazis: Pitozis gelişmiş iki olgu sunumu

Contact Info

Product

Resources

About