Odonto/Osteogenic Differentiation of Dental Pulp Stem Cells of Type 1 Diabetic Patients with Mineral Trioxide Aggregate/1α,25-Dihydroxyvitamin D3 Combination

Effects of diabetes mellitus on dental pulp: A systematic review of in vivo and in vitro studies

“…From these records, 25 were excluded by reasons available in Figure 1 and Supporting Information File 2. Finally, 21 studies met the inclusion criteria and were included for qualitative analysis (Al‐Anesi et al, 2021; Catanzaro et al, 2006; Cintra et al, 2017; Claudino et al, 2015; Cohen et al, 1963; Delle Monache et al, 2021; Ferreira et al, 2018; Garber et al, 2009; Glickman & Shklar, 1954; Ilić et al, 2012; Inagaki et al, 2010; Lee et al, 2016; Leite et al, 2008; Leite et al, 2010; Madani et al, 2014; Milosavljević et al, 2018; Müller et al, 2015; Nakajima et al, 2014; Santamaria‐Jr et al, 2021; Sugiyama et al, 2021; Uzunoglu‐Ozyurek et al, 2022).…”

“…For the in vitro category, two studies performed a cell viability assay on human dental pulp cells (hDPCs) from extracted third molars of normoglycaemic and hyperglycaemic patients (Milosavljević et al, 2018; Uzunoglu‐Ozyurek et al, 2022), whereas other four used monolayer cultures of hDPCs (Müller et al, 2015; Nakajima et al, 2014) or rat dental pulp stem cells (RDSCs) (Sugiyama et al, 2021) incubated with or without advanced glycation end products (AGEs), or exposed to methylglyoxal (MG) (Delle Monache et al, 2021). Additionally, one article performed a mineralization assessment using cultured rat dental pulp cells (RPC‐C2A) incubated with or without different levels of glucose (10, 25 and 50 mmol/L) for up to 28 days (Inagaki et al, 2010).…”

“…Out of the five studies that evaluated cell viability, mostly using MTT assay, two reported no impact of DM and AGEs on hDPCs viability (Milosavljević et al, 2018; Müller et al, 2015), while other three showed a reduction on cell viability (Delle Monache et al, 2021; Sugiyama et al, 2021; Uzunoglu‐Ozyurek et al, 2022). Three studies performed a matrix mineralization assessment (Müller et al, 2015; Sugiyama et al, 2021; Uzunoglu‐Ozyurek et al, 2022) with controversial results, which it was not sufficient to assert a conclusion.…”

“…In contrast to these data, a few evaluated in vitro studies showed that DM and AGEs might impair cell viability (Delle Monache et al, 2021; Sugiyama et al, 2021; Uzunoglu‐Ozyurek et al, 2022), while other two found no impact on cell viability (Milosavljević et al, 2018; Müller et al, 2015). These controversial data may be because the different cell lines and methods for analysis, in addition to the traditional two‐dimensional model of in vitro studies, which do not properly resemble in vivo cell environments.…”

“…Out of the five studies that evaluated cell viability, mostly using MTT assay, two reported no impact of DM and AGEs on hDPCs viability (Milosavljević et al, 2018;Müller et al, 2015), while other three showed a reduction on cell viability (Delle Monache et al, 2021;Sugiyama et al, 2021;Uzunoglu-Ozyurek et al, 2022).…”