Odontoblast stimulation in ferrets by dentine matrix components

Smith, Anthony J.; Tobias, R. S.; Cassidy, N.; Plant, C G; Browne, R.M.; Bègue-Kirn, Catherine; Ruch, J V; Lesot, Hervé

doi:10.1016/0003-9969(94)90029-9

Cited by 172 publications

(172 citation statements)

References 26 publications

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“…The biological events underlying the formation of both types of tertiary dentin are distinct, [19][20][21][22] although in both situations the end result is the secretion of the dentin matrix, yet the mechanism by which this happens is different. In the case of reparative dentinogenesis, a cascade of events involving cell division, chemotaxis, cell migration and cytodifferentiation must occur before secretion of matrix can take place.…”

Section: Introductionmentioning

confidence: 99%

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

et al. 2005

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Odontoblasts are postmitotic cells that differentiate from the dental papilla. These cells are responsible for producing the calcified dentin matrix. The pulp-odontoblast interphase contains undifferentiated mesenchymal stem cells, which have the ability to cytodifferentiate into odontoblast-like cells in response to specific signaling molecules. Dentin matrix protein 1 (DMP1) is one of the dentin noncollagenous extracellular matrix proteins that has been implicated in regulation of mineralization. In this study, we have examined the potential role of DMP1 in inducing cytodifferentiation of dental pulp stem cells into odontoblast-like cells and formation of reparative dentin in a rat model. Cavities were drilled and pulps exposed in maxillary first molars. Collagen matrix impregnated with recombinant DMP1 was implanted directly in Group 1, while calcium hydroxide, a commonly used pulp-capping agent was implanted in group 2, collagen matrix that was not impregnated with rDMP1 was implanted directly in group 3, which served as control. Each of these three groups was subdivided into two subgroups, A for 2 weeks time duration and B for 4 weeks duration. At the end of the time period the maxillae were excised, tissues were processed for histological and immunohistochemical evaluations. The results showed that DMP1 could act as a morphogen on undifferentiated mesenchymal cells present in the dentin-pulp complex. These differentiated cells had the potential to regenerate dentin-like tissue, which was confirmed by the presence of collagenous matrix, odontoblast specific markers and calcified deposits. Gene Therapy (2006) 13, 611-620.

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Section: Introductionmentioning

confidence: 99%

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

et al. 2005

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“…Morphologically, odontoblasts are columnar polarized cells with eccentric nuclei and long cellular processes aligned at the outer edges of dentin (1). After tooth eruption, reparative dentin is formed by odontoblasts in response to general mechanical erosion or disruption, and through dentinal degradation caused by bacteria (2).…”

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confidence: 99%

Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo

Gronthos

Mankani

Brahim

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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Dentinal repair in the postnatal organism occurs through the activity of specialized cells, odontoblasts, that are thought to be maintained by an as yet undefined precursor population associated with pulp tissue. In this study, we isolated a clonogenic, rapidly proliferative population of cells from adult human dental pulp. These DPSCs were then compared with human bone marrow stromal cells (BMSCs), known precursors of osteoblasts. Although they share a similar immunophenotype in vitro, functional studies showed that DPSCs produced only sporadic, but densely calcified nodules, and did not form adipocytes, whereas BMSCs routinely calcified throughout the adherent cell layer with clusters of lipidladen adipocytes. When DPSCs were transplanted into immunocompromised mice, they generated a dentin-like structure lined with human odontoblast-like cells that surrounded a pulp-like interstitial tissue. In contrast, BMSCs formed lamellar bone containing osteocytes and surface-lining osteoblasts, surrounding a fibrous vascular tissue with active hematopoiesis and adipocytes. This study isolates postnatal human DPSCs that have the ability to form a dentin͞pulp-like complex.odontoblast ͉ dentin ͉ in vivo transplantation

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“…Following primary dentinogenesis, these odontoblasts remain functional and secrete physiological secondary dentine at a continuing, reduced rate. These cells retain the ability to respond to mild environmental stimuli and upregulate their secretory activity during reactionary dentinogenesis leading to dentinal regeneration [57]. However, a more intensive stimulus may lead to death of the existing odontoblast population and, in such cases, dentin regeneration is mediated by the differentiation of a new generation of odontoblast-like cells from a precursor population during the process of reparative dentinogenesis [58].…”

Section: Dental Regenerative Therapymentioning

confidence: 99%