Odontogenic Epithelium: Immunolabeling of Ki-67, EGFR and Survivin in Pericoronal Follicles, Dentigerous Cysts and Keratocystic Odontogenic Tumors

Oliveira, Márcia Gaiger de; Lauxen, Isabel da Silva; Chaves, Anna Cecília Moraes; Rados, Pantelis Varvaki; Filho, Manoel Sant’Ana

doi:10.1007/s12105-010-0216-0

Cited by 40 publications

(42 citation statements)

References 36 publications

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“…Presented study also revealed that strong cytoplasmic and nuclear survivin staining in stromal cells was in CAs and KCOTs, but weak staining in DCs and RCs. In the article of de Oliveira et al (27), survivin staining patterns in DCs and KCOTs is also similar to our observations. Furthermore, some authors hypothesized that cytoplasmic survivin expression is responsible for poor prognosis and for the resistance to therapy in oral squamous cell carcinoma (31).…”

Section: Discussionsupporting

confidence: 92%

“…Although there are several studies related to role of these proteins in odontogenic lesions in English literature (8,9,18,24,(27)(28)(29), no comparative and comprehensive study, in which the expression of these proteins or adhesion molecules in odontogenic cystic lesions have been evaluated, to our knowledge. Therefore, we aim to evaluate the immunohistochemical expressions of CD138 (syndecan-1), CD38, E-cadherin and survivin in these cystic odontogenic lesions and investigate the potential implications of their expressions among these lesions.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical characteristics of cystic odontogenic lesions: a comparative study

Özcan

Yavan²,

Günhan³

2015

TJPATH

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Objective: Cystic ameloblastoma, keratocystic odontogenic tumor, dentigerous cyst, and radicular cyst are the most commonly encountered cystic odontogenic lesions. The aim of this study was to investigate the expressions of survivin, E-cadherin, CD138, and CD38 in these lesions and their potential diagnostic usage. Material and Method:A total of 20 cases, consisting 5 radicular cysts, 5 dentigerous cysts, 5 keratocystic odontogenic tumors and 5 cystic ameloblastomas were included in our series. For all cases, sections from the selected blocks were stained against the antibodies for survivin, E-cadherin, CD138, and CD38 on an automated device.Results: All cystic ameloblastomas and keratocystic odontogenic tumors showed diffuse and strong nuclear survivin expression. No specific survivin immunoreactivity was observed in the dentigerous and radicular cysts. E-cadherin expression was stronger in all dentigerous cysts and radicular cysts when compared to others. CD138 expression in stromal cells was prominent in cystic ameloblastomas, but gradually decreased in the other three lesions. All cases were negative for CD38. Conclusion:In the present study, loss of E-cadherin expression in epithelial cells, strong CD138 expression in stromal cells and strong nuclear survivin expression both in epithelial and stromal cells in cystic ameloblastomas and keratocystic odontogenic tumors were the most remarkable findings. These findings are also reinforced by the studies suggesting their role in the aggressiveness and pathogenesis of these tumors. Key Words: Odontogenic tumors, Odontogenic cysts, Immunohistochemistry ÖzAmaç: Kistik ameloblastoma, keratokistik odontojenik tümör, dentijeröz kist ve radiküler kist en sık karşılaşılan kistik odontojenik lezyonlardır. Çalışmada bu lezyonlarda survivin, E-cadherin, CD138 ve CD38 ekspresyonları ve bunların potansiyel tanısal kullanımları araştırılmıştır. Gereç ve Yöntem:Çalışma serimiz 5 kistik ameloblastoma, 5 keratokistik odontojenik tümör, 5 dentijeröz kist ve 5 radiküler kist olmak üzere toplam 20 olgudan oluşmaktadır. Bütün olgulara ait seçilmiş bloklardan sağlanan kesitler otomatize bir cihazda survivin, E-cadherin, CD138, and CD38 antikorları ile boyandı. Bulgular:Kistik ameloblastoma ve keratokistik odontojenik tümörlerin hepsi yaygın ve kuvvetli nükleer survivin ekspresyonu gösterdi. Dentijeröz ve radiküler kistlerde spesifik survivin ekspresyonu gözlenmedi. E-cadherin ekspresyonu diğer lezyonlarla karşılaştırıldığında, dentijeröz ve radiküler kistlerde daha belirgindi. CD138 ekspresyonu kistik ameloblastomalarda stromal hücrelerde daha belirgindi, ancak diğer lezyonlarda giderek azalan oranlardaydı. CD38 olguların hepsinde negatifdi.Sonuç: Çalışmada, keratokistik odontojenik tümörlerde ve kistik ameloblastomada epitelyal hücrelerde E-cadherin kaybı, stromal hücrelerde kuvvetli CD138 ekspresyonu ve hem epitelyal hem de stromal hücrelerde kuvvetli nükleer survivin ekspresyonu en karakteristik bulgulardır. Bu bulguların bu tür lezyonların patogenezini ve agresif davranış...

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Immunohistochemical characteristics of cystic odontogenic lesions: a comparative study

Özcan

Yavan²,

Günhan³

2015

TJPATH

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“…The results of this study confirm previously published data on survivin expression in KCOTs (Andric et al, 2010; De Oliveira et al, 2011;Leonardi et al, 2013). The study also demonstrated that survivin immunostaining is a feature of the basal and parabasal epithelial layers of KCOTs.…”

Section: Discussionsupporting

confidence: 92%

“…It is hypothesized that survivin expression is correlated with high proliferative activity in the epithelium of KCOTs as well as apoptosis inhibition (Andric et al, 2010;De Oliveira et al, 2011). Similarly, cyclin D1, a protein which regulates the transition from G1 to S phase of the cell cycle, is also expressed in KCOTs (Kimi et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Survivin, cyclin D1, and p21hras in keratocystic odontogenic tumors before and after decompression

et al. 2016

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Within the limits of the study, we can conclude that following decompression, keratocystic odontogenic tumors preserve distinct immunohistochemical profiles of cyclin D1 and p21hras expression, despite substantial reduction in size of the lesions. Significant increase of survivin expression after decompression might be attributed to higher level of epithelial proliferation caused by this procedure.

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“…Epithelial cells in KCOTs seem to have a different proliferative potential from those of other odontogenic lesions 4.…”

Section: Discussionmentioning

confidence: 92%

Keratocystic Odontogenic Tumors: Predictive Factors of Recurrence by Ki-67 and AgNOR Labelling

Selvi¹,

Tekkeşın²,

Çakarer³

et al. 2012

Int. J. Med. Sci.

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Purpose: The purpose of the present study was to investigate the possible role of Ki-67 and argyrophilic nucleolar organizing regions (AgNOR) between the recurrent and nonrecurrent keratocystic odontogenic tumors (KCOTs). Another aim was to compare the correlation between these two markers.Materials and Methods: 22 KCOTs were evaluated retrospectively. The actual proliferative activity of the KCOT was measured by Ki-67 labelling index and argyrophilic nucleolar organizing regions AgNOR count per nucleus. Results: Recurrence occurred in 3 patients (13.6%) during the follow-up period (mean follow-up, 37.8 months) The Ki-67 and AgNOR counts were significantly higher in the recurrent lesions comparing to the non-recurrent lesions. (p=0,045; p=0,049) The correlation between Ki-67 and AgNOR counts was found to be positive (r=0,853 p=0,0001).Conclusion: Within the limit of the present study, it is thought that Ki-67 and AgNOR might be helpful as a prognostic marker for the recurrences of KCOTs. These markers reinforced the meaning of the new classification of the lesion as an odontogenic tumor. Enucleation with curettage or decompression following enucleation with curettage is a simple and appropriate surgical model for the treatment of KCOT despite the relative high recurrence rate. On the other hand, the conservative treatment can be chosen only if there is no coronoid invasion, no interruptive cortical lysis and no tissular invasion.

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Odontogenic Epithelium: Immunolabeling of Ki-67, EGFR and Survivin in Pericoronal Follicles, Dentigerous Cysts and Keratocystic Odontogenic Tumors

Abstract: The aim of this study was to evaluate the biological profile of odontogenic epithelium by immunolabeling of epidermal growth factor receptor (EGFR), Ki-67 and survivin in keratocystic odontogenic tumors (KOT

Cited by 40 publications

References 36 publications

Immunohistochemical characteristics of cystic odontogenic lesions: a comparative study

Immunohistochemical characteristics of cystic odontogenic lesions: a comparative study

Survivin, cyclin D1, and p21hras in keratocystic odontogenic tumors before and after decompression

Keratocystic Odontogenic Tumors: Predictive Factors of Recurrence by Ki-67 and AgNOR Labelling

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