Odor-Driven Activity in the Olfactory Cortex of an In Vitro Isolated Guinea Pig Whole Brain With Olfactory Epithelium

Ishikawa, Tsuneo; Sato, Takaaki; Shimizu, Akira; Tsutsui, Ken; Curtis, Marco de; Iijima, Toshio

doi:10.1152/jn.01366.2005

Cited by 20 publications

(24 citation statements)

References 43 publications

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“…Olfactory feedforward inhibition is activated in the rostro-ventral portion of the aPC (aPC vr ) [40]. Notably, in insects, input synchronization via inhibition is also important for discrimination of similar odors [41].…”

Section: The Second Residue Of Helix 8 Partially Governs the Hieramentioning

confidence: 99%

Functional Role of the C-Terminal Amphipathic Helix 8 of Olfactory Receptors and Other G Protein-Coupled Receptors

Sato

Kawasaki

Mine

et al. 2016

IJMS

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G protein-coupled receptors (GPCRs) transduce various extracellular signals, such as neurotransmitters, hormones, light, and odorous chemicals, into intracellular signals via G protein activation during neurological, cardiovascular, sensory and reproductive signaling. Common and unique features of interactions between GPCRs and specific G proteins are important for structure-based design of drugs in order to treat GPCR-related diseases. Atomic resolution structures of GPCR complexes with G proteins have revealed shared and extensive interactions between the conserved DRY motif and other residues in transmembrane domains 3 (TM3), 5 and 6, and the target G protein C-terminal region. However, the initial interactions formed between GPCRs and their specific G proteins remain unclear. Alanine scanning mutagenesis of the murine olfactory receptor S6 (mOR-S6) indicated that the N-terminal acidic residue of helix 8 of mOR-S6 is responsible for initial transient and specific interactions with chimeric Gα15_olf, resulting in a response that is 2.2-fold more rapid and 1.7-fold more robust than the interaction with Gα15. Our mutagenesis analysis indicates that the hydrophobic core buried between helix 8 and TM1–2 of mOR-S6 is important for the activation of both Gα15_olf and Gα15. This review focuses on the functional role of the C-terminal amphipathic helix 8 based on several recent GPCR studies.

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Section: The Second Residue Of Helix 8 Partially Governs the Hieramentioning

confidence: 99%

Functional Role of the C-Terminal Amphipathic Helix 8 of Olfactory Receptors and Other G Protein-Coupled Receptors

Sato

Kawasaki

Mine

et al. 2016

IJMS

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“…The sensory system generates oscillatory activities between the related cortical regions and the thalamus, and the latter acts (except in the olfactory system) to gate the sensory input to the cortex and provides feedback from the cortical pyramidal neurons. In olfaction, transient oscillatory brain waves are observed in the aPC [5,[17][18][19][20][21]. Strong feed-forward inhibition [5,22,23] via the sensitive pathway from the olfactory bulb [24] and the other sensory thalamocortical circuit [25,26] or higher olfactory centers [27] could induce oscillatory brain waves that would contribute to parts of the EEGs recorded at the respective positions on the human scalp, in analogy to these experimental animals.…”

Section: A New Methods Of Real-time Estimation Of In-brain Informationmentioning

confidence: 99%

“…condition of no inputs from the nonolfactory sensory systems ( Figure 1) [1,5]. Oscillatory brain waves initiate during the 1-s odor presentation before the peak of the receptor potential, the electro-olfactogram (EOG) (the lowest trace in Figure 1) [1].…”

Section: Introductionmentioning

confidence: 99%

Wavelet Correlation Analysis for Quantifying Similarities and Real-Time Estimates of Information Encoded or Decoded in Single-Trial Oscillatory Brain Waves

Sato¹,

Kajiwara²,

Takashima³

et al. 2018

Wavelet Theory and Its Applications

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Even in cases when we recognize identical objects or when we behave similarly, the spatiotemporal activities in the brain are likely to fluctuate to various degrees. Temporally fluctuating responses easily decrease by averaging replicate measures. We previously developed a wavelet correlation analysis that tolerates the across-trial oscillatory phase variability observed in odor-induced cortical responses. The wavelet correlation analysis revealed a change in the neuronal information redundancy of transient and oscillatory brain waves from the dependencies on stimulus experience (high redundancy) to stimulus quality (low redundancy) between the input and output layers of the anterior piriform cortex in guinea pigs. We report on its application to estimate information in the fine temporal structures of single-trial brain waves. By using a set of standard brain waves for each information in a given category, the highest wavelet correlation coefficients provided the first candidate of estimated information with 75% accuracy. Moreover, the probability of including the correct information for the two upper candidates, regardless of information redundancy of the signal sources, was >92%. The wavelet correlation analysis is useful for similarity analyses and real-time estimates of in-brain information and for its application to brain-machine interfaces or medical/research tools.

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“…We modified the procedure reported by de Curtis et al . (1998) for maintaining the nose attached to the brain for odor stimulation of the sensory organ (Iijima et al ., 2000; Ishikawa et al ., 2007). Lactate was added to the artificial plasma solution as another energy source to facilitate the recovery of synaptic function (Fox et al ., 1988; Schurr et al ., 1997, 1999; Fornai et al ., 2000).…”

Section: Methodsmentioning

confidence: 99%

“… Convergent and overlapped inputs of odorant receptor (OR) signals to the olfactory centers via two possible pathways (modified from Ekstrand et al ., 2001; Zou et al ., 2001): a possible feedforward inhibition (Ishikawa et al ., 2007) and a higher firing rate of the middle tufted cells compared to the mitral cells (Nagayama et al ., 2004). Genetic tracing indicated clusters of pyramidal cells receiving M5 OR signals (blue) and M50 OR signals (red) from the olfactory bulb to the entorhinal cortex in mice.…”

Section: Introductionmentioning

confidence: 99%

Architecture of odor information processing in the olfactory system

Sato

Hirono

Hamana

et al. 2008

Anatomical Science International

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Since the discovery of the superfamily of approximately 1000 odorant receptor genes in rodents, the structural simplicity as well as the complexity of the olfactory system have been revealed. The simple aspects include the one neuron-one receptor rule and the exclusive convergence of projections from receptor neurons expressing the same receptors to one or two glomeruli in the olfactory bulb. Odor decoding in the olfactory cortex or higher cortical areas is likely to be a complicated process that depends on the sequence of signal activation and the relative signal intensities of receptors overlapping for similar but different odors. The aim of the present study was to investigate odor information processing both in receptors and in the olfactory cortex. At the receptor level, the similarity and difference in receptor codes between a pair of chiral odorants were examined using the tissue-printing method for sampling all the epithelial zones. In order to dissect odor-driven signal processing in the olfactory cortex by reducing cross-talk with the non-olfactory activities, such as cyclic respiration or other sensory inputs, an in vitro preparation of isolated whole brain with an attached nose was developed, and the methodologies and resulting hypothesis of receptor-sensitivity-dependent hierarchical odor information coding were reviewed.

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Odor-Driven Activity in the Olfactory Cortex of an In Vitro Isolated Guinea Pig Whole Brain With Olfactory Epithelium

Abstract: doi: 10.1152/jn.01366.2005 You might find this additional info useful... This article cites 46 articles, 18 of which you can access for free at

Cited by 20 publications

References 43 publications

Functional Role of the C-Terminal Amphipathic Helix 8 of Olfactory Receptors and Other G Protein-Coupled Receptors

Functional Role of the C-Terminal Amphipathic Helix 8 of Olfactory Receptors and Other G Protein-Coupled Receptors

Wavelet Correlation Analysis for Quantifying Similarities and Real-Time Estimates of Information Encoded or Decoded in Single-Trial Oscillatory Brain Waves

Architecture of odor information processing in the olfactory system

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