Odorant-binding protein-based identification of natural spatial repellents for the African malaria mosquito Anopheles gambiae

Kröber, Thomas; Koussis, Konstantinos; Bourquin, Martine; Tsitoura, Panagiota; Κωνσταντοπούλου, Μαρία; Awolola, T. S.; Dani, Francesca Romana; Qiao, Hui; Pelosi, Paolo; Iatrou, Kostas; Guérin, Patrick M.

doi:10.1016/j.ibmb.2018.03.008

Cited by 28 publications

(28 citation statements)

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“…Albopictus, in 5-fold-lower doses to DEET (Giatropoulos et al, 2018) and An. Gambiae (Kröber et al, 2018). Their respective larvicidal bioassays results are summarized in Table 5, verifying that the specific EO displays…”

Section: Dose Response and Formulationsupporting

confidence: 60%

From Bio-Prospecting to Field Assessment: The Case of Carvacrol Rich Essential Oil as a Potent Mosquito Larvicidal and Repellent Agent

et al. 2018

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Contemporary legislation tends to increase limitation on the use of all synthetic pesticides, promoting bio-pesticides as a safer alternative. Bio-prospecting efforts for bio-pesticides provide results, which rarely reach the industry. Present essay elaborates on our efforts to chart the path from the laboratory bench to field assessment. Eight Mediterranean wild gathered foods provided the essential oils that were assessed as mosquito control agents against the Asian tiger mosquito (Aedes albopictus). Three Lamiaceae essential oils, derived from Satureja thymbra, Origanum onites, and Thymbra spicata presented carvacrol as principal component. All exhibited DEET-like repellent performance and total larvae mortality defining the carvacrol rich essential oil (CREO) as a promising mosquito control agent. A commercial variety of Origanum vulgare ssp. hirtum, was selected as CREO source and subjected to dose-response and eco-toxicity studies. We have found significant larvicidal (LC 90 of 58,747 mg/L), and repellent (0.2 µL/cm 2) properties, but also severe toxicity (LC 90 of 12,806 mg/L) against Macrocyclops albidus. This last figure was the limit for the larvicidal field assessment; while for the repellent evaluation was used double the minimum indication (0.4 µL/cm 2). CREO was tested per se as larvicidal agent, and emulsified for both repellent and larvicidal field activity. The emulsified CREO's spatial repellent assessment showed maximum efficacy of 86% in day 1 that gradually declined in the following 2 days (81%, 69%). Both emulsified and crude CREO proved to be efficient larvicidal agents, with crude CREO (3 weeks) overrunning slightly the emulsified (2 weeks) in terms of endurance. Conclusively, CREO in its emulsified form may be considered as a promising mosquito larvicidal and repellent agent, applicable in both precautionary and emergency response measures.

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Section: Dose Response and Formulationsupporting

confidence: 60%

From Bio-Prospecting to Field Assessment: The Case of Carvacrol Rich Essential Oil as a Potent Mosquito Larvicidal and Repellent Agent

et al. 2018

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“…gambiae and Culex spp. mosquitoes (33), raised the question of whether these as well as the new antagonists identified in this study were also active against Aedes, the third major mosquito genus of the Culicidae family, which comprises multiple haematopagous species and infectious disease vectors. The initial testing of two selected antagonists, CRV and OCT, in X. laevis oocytes expressing ORco of Ae.…”

Section: Discussionmentioning

confidence: 94%

“…Such effects had been noted previously in our studies on laboratory and field populations of An. gambiae and Culex spp (33). The enhanced bioactivities of binary mixtures of chemically diverse AgamORco antagonists raised the question of the possible relevance of antagonist binding sites.…”

Section: Discussionmentioning

confidence: 99%

“…albopictus very similar to DEET (landing inhibition of 93.1% versus 96.7%), was previously shown to repel An. gambiae and Culex spp mosquitoes in the field more efficiently than DEET (33). A ternary mixture consisting of 0.0033 μL/cm 2 each of CRV (21.7 nmole/cm 2 ), CA (21.7 nmole/cm 2 ) and OCT (23.3 nmole/cm 2 ), displayed repellence activity of 91.1% against Ae.…”

Section: Binary Mixtures Of Orco Antagonists Are More Active Than Sinmentioning

confidence: 99%

“…In previous studies, we have used multiple OBPs of the African malaria mosquito vector Anopheles gambiae (AgamOBPs; [26][27][28][29][30][31] as screening tools for the discovery of natural volatile organic compounds (VOCs) capable of modifying olfactory-mediated behaviors (32,33). This effort resulted in the identification of natural compounds with strong repellent activities against both Anopheles and Culex mosquitoes (33) suggesting the existence of phylogenetically conserved molecular mechanisms and behavioral outputs in mosquitoes. Further studies revealed that the most potent of the identified repellents acted as allosteric inhibitors of multiple AgamORs in the heteromeric complexes and blocked odorant-specific responses by interacting directly with AgamORco (34).…”

Section: Introductionmentioning

confidence: 99%

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Volatile allosteric antagonists of mosquito odorant receptors inhibit normal odor-dependent behaviors

Kythreoti

Sdralia

Tsitoura

et al. 2020

Preprint

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Odorant-dependent behaviors in insects are triggered by the binding of odorant ligands to the variable subunits of heteromeric olfactory receptors. Previous studies have shown, however, that specific odor binding to ORco, the common subunit of odorant receptor heteromers, may alter allosterically olfactory receptor function and affect profoundly subsequent behavioral responses. Here we report on the identification of several antagonists of the odorant receptor co-receptor of the African malaria vector Anopheles gambiae, AgamORco, in a small collection of natural volatile organic compounds (VOCs) using a relevant insect cell-based screening platform. Because some of the identified antagonists were previously shown to strongly repel Anopheles and Culex mosquitoes, here we examined the bioactivities of the identified antagonists against Aedes, the third major genus of the Culicidae family. The tested antagonists were found to inhibit the function of Ae. aegypti ORco ex vivo and repel Asian tiger, Ae. albopictus, adult mosquitoes. Specific antagonist binary mixtures elicited higher repellency than single antagonists. Binding competition assays suggested antagonist binding to distinct ORco sites as a likely cause for the enhanced repellence of the blends. These findings demonstrate that a simple screening assay may be used for the identification of allosteric modifiers of olfactory-driven 2 behaviors capable of providing enhanced indoor and outdoor protection against multiple mosquito borne infectious diseases.

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Host plant recognition by two odorant‐binding proteins in Rhynchophorus ferrugineus (Coleoptera: Curculionidae)

Huang

Hou

2023

Pest Management Science

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BACKGROUNDRhynchophorus ferrugineus, the red palm weevil (RPW), is a key pest that attacks many economically important palm species and that has evolved a sensitive and specific olfactory system to seek palm hosts. Odorant‐binding proteins (OBPs) not only play crucial roles in its olfactory perception process but are also important molecular targets for the development of new approaches for pest management.RESULTSAnalysis of the tissue expression profiles of RferOBP8 and RferOBP11 revealed that these two Rhynchophorus ferrugineus odorant binding proteins (RferOBPs) exhibited high expression in the antennae and showed sexual dimorphism. We analyzed the volatiles of seven host plants by gas chromatography–mass spectrometry and screened 13 potential ligands by molecular docking. The binding affinity of two recombinant OBPs to aggregation pheromones and 13 palm odorants was tested by fluorescence competitive binding assays. The results revealed that eight tested palm volatiles and ferrugineol have high binding affinities with RferOBP8 or RferOBP11. Behavioral trials showed that these eight odor compounds could elicit an attraction response in adult RPW. RNA interference analysis indicated that the reduction in the expression levels of the two RferOBPs led to a decrease in behavioral responses to these volatiles.CONCLUSIONThese results suggest that RferOBP8 and RferOBP11 are involved in mediating the responses of RPW to palm volatiles and to aggregation pheromones and may play important roles in RPW host‐seeking. This study also provides a theoretical foundation for the promising application of novel molecular targets in the development of new behavioral interference strategies for RPW management in the future. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Odorant-binding protein-based identification of natural spatial repellents for the African malaria mosquito Anopheles gambiae

Cited by 28 publications

References 94 publications

From Bio-Prospecting to Field Assessment: The Case of Carvacrol Rich Essential Oil as a Potent Mosquito Larvicidal and Repellent Agent

From Bio-Prospecting to Field Assessment: The Case of Carvacrol Rich Essential Oil as a Potent Mosquito Larvicidal and Repellent Agent

Volatile allosteric antagonists of mosquito odorant receptors inhibit normal odor-dependent behaviors

Host plant recognition by two odorant‐binding proteins in Rhynchophorus ferrugineus (Coleoptera: Curculionidae)

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