Odorant Representations Are Modulated by Intra- but Not Interglomerular Presynaptic Inhibition of Olfactory Sensory Neurons

McGann, John P.; Pírez, Nicolás; Gainey, Melanie; Muratore, Christina; Elias, Adam S.; Wachowiak, Matt

doi:10.1016/j.neuron.2005.10.031

Cited by 179 publications

(203 citation statements)

References 39 publications

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“…Release probability at OSN terminals is strongly regulated by GABAergic and dopaminergic intraglomerular feedback operating via presynaptic GABAB and D 2 receptors, respectively (Hsia et al, 1999;Aroniadou-Anderjaska et al, 2000;McGann et al, 2005). To ensure that the lack of maturation we saw in PPR was not attributable to developmental differences in this intrinsic presynaptic modulation, we recorded 50 ms ISI PPR before and after washin of the GABA B antagonist CGP-55845 (CGP; 5 M) and the D 2 antagonist sulpiride (sul; 100 M).…”

Section: Quantal Size Does Not Change With Maturation In Any Cell Groupmentioning

confidence: 99%

Functional Maturation of the First Synapse in Olfaction: Development and Adult Neurogenesis

Grubb

Nissant²,

Murray³

et al. 2008

J. Neurosci.

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The first synapse in olfaction undergoes considerable anatomical plasticity in both early postnatal development and adult neurogenesis, yet we know very little concerning its functional maturation at these times. Here, we used whole-cell recordings in olfactory bulb slices to describe olfactory nerve inputs to developing postnatal neurons and to maturing adult-born cells labeled with a GFP-encoding lentivirus. In both postnatal development and adult neurogenesis, the maturation of olfactory nerve synapses involved an increase in the relative contribution of AMPA over NMDA receptors, and a decrease in the contribution of NMDA receptors containing the NR2B subunit. These postsynaptic transformations, however, were not mirrored by presynaptic changes: in all cell groups, paired-pulse depression remained constant as olfactory nerve synapses matured. Although maturing cells may therefore offer, transiently, a functionally distinct connection for inputs from the nose, presynaptic function at the first olfactory connection remains remarkably constant in the face of considerable anatomical plasticity.

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Section: Quantal Size Does Not Change With Maturation In Any Cell Groupmentioning

confidence: 99%

Functional Maturation of the First Synapse in Olfaction: Development and Adult Neurogenesis

Grubb

Nissant²,

Murray³

et al. 2008

J. Neurosci.

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show abstract

“…Intriguingly, results from this imaging study were different from those obtained using optical imaging or 2-deoxyglucose analysis, possibly because these methods are lower resolution, requiring greater signal averaging within and between animals, or because these methods may be more sensitive to post-synaptic activity (rather than presynaptic release). These animals have also been used to test hypotheses about inter-and intra-glomerular inhibition in vitro and in vivo [29].…”

Section: Phluorins Ph-based Sensorsmentioning

confidence: 99%

Visualizing circuits and systems using transgenic reporters of neural activity

Barth

2007

Current Opinion in Neurobiology

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Genetically encoded sensors of neural activity enable visualization of circuit-level function in the central nervous system. Although our understanding of the molecular events that regulate neuronal firing, synaptic function, and plasticity has expanded rapidly over the past fifteen years, an appreciation for how cellular changes are functionally integrated at the circuit level has lagged. A new generation of tools that employ fluorescent sensors of neural activity promises unique opportunities to bridge the gap between cellular and system-levels analysis.This review will focus on genetically-encoded sensors. A primary advantage of these indicators is that they can be non-selectively introduced to large populations of cells using either transgenic or viral-mediated approaches. This ability removes the non-trivial obstacles of how to get chemical indicators into cells of interest, a problem that has dogged investigators who have been interested in mapping neural function in the intact CNS. Five different types of approaches and their relative utility will be reviewed here: 1) reporters of immediate-early gene (IEG) activation using promoters such as c-fos and arc, 2) voltage-based sensors, such as GFPcoupled Na + and K + channels, 3) Cl − based sensors, 4) Ca ++ -based sensors, such as Camgaroo and the troponin-based TN-L15, and 5) pH-based sensors, which have been particularly useful for examining synaptic activity of highly convergent afferents in sensory systems in vivo. Particular attention will be paid to reporters of IEG expression, since these tools employ the built-in threshold function that occurs with activation of gene expression, provoking new experimental questions by expanding the timescale of analysis for circuit and system-level functional mapping. Fluorescent reporters of inducible gene expressionImmediate-early gene expression (IEG) can be a reliable marker of elevated neuronal firing, reporting activity over time scales that range from minutes to hours compared to ms and seconds for voltage-or ion-based sensors. In ion-based approaches, indicators are constitutively present in the cell, and the fluorescent protein must be engineered to be a sensor with rapid onset and offset kinetics to achieve temporal fidelity with the process being measured. In contrast, monitoring IEG expression can be directly coupled to transcription of the reporter gene. However, because maturation of the fluorophore is required for detection, a temporal delay may be introduced into reporter detection. This delay in reporting activity is advantageous in that it expands the types of experimental questions that can be addressed. For example, do IEGexpressing cells remain more excitable after stimulus cessation? Are cells activated by two temporally distinct stimuli (i.e. training and recall in a memory task) overlapping or distinct?

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“…While the rise in fluorescence is caused by vesicular release at olfactory nerve terminals, we do not have yet an explanation for the transient dip. Several hypotheses could be proposed, going from activity dependant changes in fluorescent light absorption by the tissue (intrinsic signal), to transient changes in extracellular acidity due to release as observed in slice experiments (McGann et al, 2005; with the restriction that the latter phenomenon seems faster and should be more spatially restricted than the transient dip). Nevertheless it was clear that a linear model of SpH signal should have four components: resting fluorescence level, photobleaching, odour induced fluorescence rise and transient dip (Fig.…”

Section: Sph Signalmentioning

confidence: 99%

Wavelet-based multi-resolution statistics for optical imaging signals: Application to automated detection of odour activated glomeruli in the mouse olfactory bulb

Bathellier¹,

Ville²,

Blu³

et al. 2007

NeuroImage

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Odorant Representations Are Modulated by Intra- but Not Interglomerular Presynaptic Inhibition of Olfactory Sensory Neurons

Cited by 179 publications

References 39 publications

Functional Maturation of the First Synapse in Olfaction: Development and Adult Neurogenesis

Functional Maturation of the First Synapse in Olfaction: Development and Adult Neurogenesis

Visualizing circuits and systems using transgenic reporters of neural activity

Wavelet-based multi-resolution statistics for optical imaging signals: Application to automated detection of odour activated glomeruli in the mouse olfactory bulb

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