Oesophagostomum radiatum: Successful vaccination of calves with an extract of in vitro cultured larvae

East, I.J.; Berrie, D.A.; Fitzgerald, C. J.

doi:10.1016/0020-7519(88)90047-1

Cited by 18 publications

(5 citation statements)

References 11 publications

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“…Three-month-old calves injected with homogenates of L4 larvae were protected 85% against oral challenge (30). A saline-soluble fraction from larvae cultured to the L4 stage in vitro, injected sub-cutaneously in Freund's adjuvant induced circulating specific antibodies and gave a 99% reduction in adult worm burden when used at a dose of 2 mg total protein per calf and a reduction of 80% at 0.4 mg per calf (17). The fraction clearly contains a mixture of proteins and by comparison with the Trichinella studies it may be calculated that essentially total protection could be obtained with between 10 and 20 ug of a purified antigen.…”

Section: Antigens Identified By Experimentally Induced Antibodiesmentioning

confidence: 99%

Strategies for vaccination against gastro-intestinal nematodes

Smith¹,

Munn²

1990

Rev. Sci. Tech. OIE

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A great diversity of gastro-intestinal nematodes parasitise man and his animals. Each nematode is antigenically highly complex with distinct developmental stages. The parasites occupy a range of niches in the host gut and have a variety of feeding habits. Hosts have evolved only partially successful local immune responses. The host and parasite factors modulating these responses are described.Strategies for vaccination are surveyed. It is argued that the best route to successful vaccines will be to seek parasite immunogens which are not normally seen by the host's immune system in the course of infection. Routes to be followed in identifying and characterising such immunogens are proposed and methods for evaluating and maximising the immune response are surveyed. The success of this strategy is illustrated by reference to an experimental molecular vaccine against Haemonchus contortus. Strategies for the use of vaccines and methods for their production in vitro are reviewed.

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Section: Antigens Identified By Experimentally Induced Antibodiesmentioning

confidence: 99%

Strategies for vaccination against gastro-intestinal nematodes

Smith¹,

Munn²

1990

Rev. Sci. Tech. OIE

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“…The in vitro culture technique for O. radiatum has been described in detail previously (EAST et al, 1988). In some experiments, the 50% foetal calf serum or the 50% NCTC 135 was replaced by alternative sera or media as indicated in the text.…”

Section: Culturementioning

confidence: 99%

“…The ELISA assay was done by the method of EAST et al (1988). Serial 1 in two dilutions of an initial 1/100 dilution of the various sera were assayed for antics radiatum antibody.…”

Section: Elisamentioning

confidence: 99%

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Oesophagostomum radiatum: inhibition of in vitro development by newborn calf serum

East

Fitzgerald

1990

J. Helminthol.

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Oesophagostomum radiatum developed to fourth stage larvae after 14 days in in vitro culture. However, development was totally inhibited if the standard 50% foetal calf serum in the medium was replaced by newborn calf serum. Inhibition did not occur with serum from cattle immune to O. radiatum through natural infection or experimental vaccination irrespective of the titre of specific antibodies to O. radiatum in each serum. The inhibitory action of NCS could be abolished by heat treatment at 56°C for 1 h but not by dialysis or repeated freeze-thawing. The inhibition was not consistent with observed differences in the activity of 19 enzymes in the various sera or the absence of various thiol-containing stimulants of worm development.

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“…este es un parásito importante que también infecta a humanos y tiene un amplio potencial zoonótico debido a su gran distribución (Li et al, 2017); pertenece a la superfamilia Strongyloidea y es un nematodo de gran importancia económica a nivel mundial(Tyagi et al, 2015). Se han inmunizado terneros con diferentes dosis de extracto soluble de larvas de Oesophagostomum radiatum cultivadas in vitro, con esta metodología se observó una reducción del 81% y 99% en la carga de nematodos adultos en intestino, y disminuciones en FEC en un 75% y 100%, a dosis bajas o altas de este extracto, respectivamente(East et al, 1988). Si bien existen grandes avances científicos, a lo largo de los años, sustentados en(Hill et al, 1994).…”

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Las generaciones de las vacunas: Caso de vacunas antiparasitarias gastrointestinales utilizadas en Medicina Veterinaria

Prieto

Borda

Hernández

2022

Rev Sist Prod Agroecol.

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Las vacunas son el pilar fundamental de la medicina preventiva y la base para posibles planes de control y/o erradicación de enfermedades, especialmente las infecciosas. Los parásitos internos en los animales de producción y de compañía continúan siendo una de las principales amenazas para la salud y el bienestar animal con importantes implicaciones económicas, además de su impacto en la salud pública mundial. Su control se ha basado casi exclusivamente en fármacos quimioterápicos, que desde hace varios años han perdido su eficacia y existen claros ejemplos de resistencia parasitaria a ellos. Hay pocos ejemplos comerciales de vacunas de parásitos gastrointestinales disponibles comercialmente para su uso en la práctica de la Medicina Veterinaria. Esta revisión describe algunos ejemplos comerciales de vacunas gastrointestinales antiparasitarias para su formulación en la práctica médica veterinaria, visto desde la perspectiva de “las generaciones de vacunas” y respaldado por estudios clínicos experimentales de antígenos prometedores para el control profiláctico de ciertos agentes parasitarios gastrointestinales de interés en salud pública principalmente. Hasta la fecha, está disponible con ciertas limitaciones comerciales en algunos países europeos y oceánicos Barbervax® y en países sudamericanos Providean® Hidatil EG95 para uso en rumiantes para el control de Haemonchus contortus y Echinococcus granulosus, respectivamente; en algunos países de América y África, Cysvax™ está disponible para el control de Taenia solium en cerdos; y en el mundo con muy pocas limitaciones, una serie de vacunas comerciales para el control de la coccidosis como la Eimeria spp. en la industria avícola: pavos, pollos de engorde y gallinas ponedoras (ej: CocciVac®, Immucox®, Paracox®, entre otros). Existe la necesidad de tener estos tipos de vacunas en todos los países donde estos parásitos gastrointestinales son endémicos y de esta manera brindar opciones para su control, por consiguiente, una serie de inversiones económicas son necesarias para apoyar el desarrollo técnico-científico en torno al desarrollo de nuevos biológicos (nueva generaciones de vacunas) efectivos y seguros para el control de los parásitos internos más relevantes en animales de producción y de compañía.

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Oesophagostomum radiatum: Successful vaccination of calves with an extract of in vitro cultured larvae

Cited by 18 publications

References 11 publications

Strategies for vaccination against gastro-intestinal nematodes

Strategies for vaccination against gastro-intestinal nematodes

Oesophagostomum radiatum: inhibition of in vitro development by newborn calf serum

Las generaciones de las vacunas: Caso de vacunas antiparasitarias gastrointestinales utilizadas en Medicina Veterinaria

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