Oestradiol and progesterone differentially alter cytoskeletal protein expression and flame cell morphology in Taenia crassiceps

Ambrosio, Javier R.; Ostoa‐Saloma, Pedro; Palacios-Arreola, Margarita Isabel; Ruíz-Rosado, Azucena; Sánchez-Orellana, Pedro L.; Reynoso-Ducoing, Olivia; Nava-Castro, Karen Elizabeth; Martínez-Velázquez, Nancy; Escobedo, Galileo; Ibarra-Coronado, Elizabeth; Valverde-Islas, Laura; Morales‐Montor, Jorge

doi:10.1016/j.ijpara.2014.04.004

Cited by 16 publications

(9 citation statements)

References 39 publications

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“…The effect could be mediated through a specific estrogen receptor that promotes the expression of c-fos and c-jun (AP-1 transcriptional complex) and suggests that E2 has proliferative effects on the parasite [92]. Our laboratory also demonstrates that exposure of T. crassiceps cysticerci to E2 and P4 induced differential protein expression patterns regarding to changes in actin, tubulin and myosin expression altering flame cells at the level of the ciliary tuft [93]. In contrast T4 and DHT induced 90% of mortality caused by an alteration in the function of flame cells, without changes in actin, tubulin or myosin expression [94].…”

Section: Hormones Immune Response and Parasitic Infectionmentioning

confidence: 57%

Endocrine Immune Interactions in the Host-Parasite Relationship: Steroid Hormones as Immune Regulators in Parasite Infections

H¹,

KE²

2015

J Steroids Hormon Sci

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Section: Hormones Immune Response and Parasitic Infectionmentioning

confidence: 57%

Endocrine Immune Interactions in the Host-Parasite Relationship: Steroid Hormones as Immune Regulators in Parasite Infections

H¹,

KE²

2015

J Steroids Hormon Sci

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“…This characteristic has facilitated the search for molecules with potential pharmacological, diagnostic, or immunotherapeutic value against parasitic diseases [11, 12]. Owing to its morphological and cellular similarity to T. solium, T. crassiceps has enabled the successful characterization of cytoskeletal proteins and changes to their expression pattern in response to antihelminthic drugs [13, 14] or other potential antiparasitic chemicals [15].…”

Section: Introductionmentioning

confidence: 99%

In VitroAnalyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci

Ríos-Valencia

López-Villegas

Chiguer

et al. 2019

Journal of Parasitology Research

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Cytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. The aim of this work was to determine the presence of a septin-like protein in cysticerci of Taenia crassiceps and Taenia solium using the deduced amino acid sequence of T. solium septin 4 (SEPT4_Tsm), to design and synthesize a derived immunogenic peptide (residues 88 to 103), to prepare a specific rabbit polyclonal antibody, and to examine the effects of FCF at different concentrations and exposure times on an in vitro culture of T. crassiceps cysticerci. In vitro, FCF altered the morphology and motility of T. crassiceps cysticerci, and its effects were reversible under specific concentrations. In addition, we observed by ultrastructural observation that FCF alters the cellular subunit of the protonephridial system of cestodes, where disruption of the axoneme pattern of flame cells was observed. The rabbit polyclonal antibody prepared against the synthetic peptide recognized a major band of 41 kDa in both parasites. Our results establish the importance of SEPT4_Tsm in the dynamics and survival of taeniid cysticerci, as well as their susceptibility to FCF. This is also the first report that a septin is present in the cytoskeleton of taeniids.

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“…[ 16 ]. Likewise, P 4 treatment increases cytoskeleton protein expression including actin, tubulin and myosin in T. crassiceps , all prominent components of flame cells belonging to the parasite excretory system [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

A novel progesterone receptor membrane component (PGRMC) in the human and swine parasite Taenia solium: implications to the host-parasite relationship

et al. 2018

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BackgroundWe have previously reported that progesterone (P4) has a direct in vitro effect on the scolex evagination and growth of Taenia solium cysticerci. Here, we explored the hypothesis that the P4 direct effect on T. solium might be mediated by a novel steroid-binding parasite protein.MethodsBy way of using immunofluorescent confocal microscopy, flow cytometry analysis, double-dimension electrophoresis analysis, and sequencing the corresponding protein spot, we detected a novel PGRMC in T. solium. Molecular modeling studies accompanied by computer docking using the sequenced protein, together with phylogenetic analysis and sequence alignment clearly demonstrated that T. solium PGRMC is from parasite origin.ResultsOur results show that P4 in vitro increases parasite evagination and scolex size. Using immunofluorescent confocal microscopy, we detected that parasite cells showed expression of a P4-binding like protein exclusively located at the cysticercus subtegumental tissue. Presence of the P4-binding protein in cyst cells was also confirmed by flow cytometry. Double-dimension electrophoresis analysis, followed by sequencing the corresponding protein spot, revealed a protein that was previously reported in the T. solium genome belonging to a membrane-associated progesterone receptor component (PGRMC). Molecular modeling studies accompanied by computer docking using the sequenced protein showed that PGRMC is potentially able to bind steroid hormones such as progesterone, estradiol, testosterone and dihydrodrotestosterone with different affinities. Phylogenetic analysis and sequence alignment clearly demonstrated that T. solium PGRMC is related to a steroid-binding protein of Echinoccocus granulosus, both of them being nested within a cluster including similar proteins present in platyhelminths such as Schistocephalus solidus and Schistosoma haematobium.ConclusionProgesterone may directly act upon T. solium cysticerci probably by binding to PGRMC. This research has implications in the field of host-parasite co-evolution as well as the sex-associated susceptibility to this infection. In a more practical matter, present results may contribute to the molecular design of new drugs with anti-parasite actions.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-2703-1) contains supplementary material, which is available to authorized users.

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Oestradiol and progesterone differentially alter cytoskeletal protein expression and flame cell morphology in Taenia crassiceps

Cited by 16 publications

References 39 publications

Endocrine Immune Interactions in the Host-Parasite Relationship: Steroid Hormones as Immune Regulators in Parasite Infections

Endocrine Immune Interactions in the Host-Parasite Relationship: Steroid Hormones as Immune Regulators in Parasite Infections

In VitroAnalyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci

A novel progesterone receptor membrane component (PGRMC) in the human and swine parasite Taenia solium: implications to the host-parasite relationship

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