Oestrogen-Like and Anxiolytic Properties of Pueraria phaseoloides Extracts Onovariectomized Wistar Rats

Abstract

Studies carried out on leguminous plants of the genus Pueraria showed that some species of this genus are endowed with oestrogenic properties and are able to relieve some menopausal symptoms. The aim of thiswork was to assess the potential health benefit of Pueraria phaseoloides in ovariectomized rats, an experimental model of menopause. Ovariectomized Wistar rats were treated for 3-days with aqueous (AE) and dichloromethane/methanol (DCM/MeOH) extracts of Pueraria phaseoloides, following the 14-days interval necessary for functionally perceptible oestrogen decline. Oestrogen-like effects were assessed on primary oestrogen target organs, while anxiolytic properties were evaluated with help of the elevated plus-maze (EPM) and the open field (OF) tests. Then, the effects of extracts on hot flushes were evaluated using data loggers. The DCM/MeOH extract (dose 500 mg/kg) significantly increased the uterine epithelial thickness (p < 0.05). Both AE and DCM/MeOH extracts induced the differentiation of the acini, and led to an increase in eosinophilic secretary granules in mammary gland sat all doses tested. Pueraria phaseoloides induced an increase in the percentages of time spent and number of entries in the open arms of the EPM, in the time spent at the centre of the arena in the OF test. The most pronounced anxiolytic effects were induced by AE at the doses 150 and 300 mg/kg. Instead, the DCM/MeOH extract had the strongest oestrogen-like effects and reduced significantly the total number (p < 0.05, p < 0.01), the frequency of hot flushes (at all the doses tested), and their average duration at 300 mg/kg (p < 0.01). Our results suggest that Pueraria phaseoloides has oestrogenic properties and can correct various physiological alterations related to oestrogen depletion in ovariectomized rats, possibly through oestrogenic pathway.

Efficacy of Non-Soy Isoflavone (Pueraria phaseoloides) on The Symptoms Severity Scoring by Kupperman Index in Menopausal Women

“…Isoflavones are being used for the alternative' natural' management of menopausal symptoms, which are analogs of 17-estradiol and can bind both estrogen receptors  (ER) and  (ER) [7]. One of the plantderived isoflavones was isolated from non-soy Tropical kudzu or Tunggak bean, Pueraria phaseoloides which compounds estrogenic properties, such as miroestrol, puerarin, deoxymireostrol, kwakhurin, and among coumestrol class [5].…”

“…Isoflavones have already been isolated from Pueraria species which are not soy. The genus of Pueraria is a structural analog of estrogen 17-estradiol in the human body that is useful for alternative therapy to treat menopausal symptoms [4,7]. Tropical kudzu or Tunggak bean, Pueraria phaseoloides was used in Chinese herbal medicine that traced back to century-old.…”