Oestrogen Responses of Rats Neonatally Sterilized With Steroids

Wrenn, T.R.; Wood, Joan R.; Bitman, Joel

doi:10.1677/joe.0.0450415

Cited by 27 publications

(15 citation statements)

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“…Our data on androgenized female rats are consistent with those of previous studies (1)(2)(3)(4)(5)(11)(12). Our findings suggest the animals had altered ovulation and even an absence of ovulation.…”

supporting

confidence: 92%

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Effects of metformin on the reproductive system of androgenized female rats

Mahamed

Maganhin

Simões

et al. 2011

Fertility and Sterility

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supporting

confidence: 92%

“…Afterward, the animals were evaluated through vaginal smears collected for 4 days. The animals medicated with testosterone propionate had a closed vagina (1)(2)(3)5).…”

mentioning

confidence: 99%

Effects of metformin on the reproductive system of androgenized female rats

Mahamed

Maganhin

Simões

et al. 2011

Fertility and Sterility

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“…Another group of nine androgenized and ten oestrous females were bilaterally ovariectomized. Six days later, one uterine horn was excised and weighed and oestrogen administered as above.This experiment confirms the findings of Wrenn, Wood & Bitman (1969) that the initial weight of the uterus is similar in normal dioestrous female (NF) and in androgenized female (AF) rats (NF = 113-9± 11-4 (s.e.m.) mg; AF = 114-4 + 4-9).…”

supporting

confidence: 91%

Oestrogen-Induced Proteins and Uterine Growth in the Androgenized Female Rat

Lobl¹,

Trotta²,

Brumberger³

1974

Journal of Endocrinology

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Administration of testosterone propionate (TP) to neonatal female rats induces a syndrome of sterility, characterized by acyclicity and anovulation (Barraclough & Gorski, 1961;Gorski, 1966;Lobi & Gorski, 1969). This study shows the ability of the uterus of androgenized female rats to respond to oestradiol by change in weight and by formation of specific oestrogen-induced proteins (IP) (Barnea & Gorski, 1970).To study the response of the uterus to oestradiol, groups of 15 normal dioestrous and 15 androgenized (1\m=.\25mg TP s.c. on postnatal day 3) adult female rats were hemihysterectomized (ipsilateral ovary removed simultaneously) and the excised uterine tissue was cleaned and weighed. These animals were then injected twice daily with 4 \g=m\g oestradiol benzoate for 5 days. On the 6th day, all animals were killed and the remaining uterine horn was excised, cleaned and weighed. Another group of nine androgenized and ten oestrous females were bilaterally ovariectomized. Six days later, one uterine horn was excised and weighed and oestrogen administered as above.This experiment confirms the findings of Wrenn, Wood & Bitman (1969) that the initial weight of the uterus is similar in normal dioestrous female (NF) and in androgenized female (AF) rats (NF = 113-9± 11-4 (s.e.m.) mg; AF = 114-4 + 4-9). The uterus of the normal female responded to oestradiol treatment by a 62% increase in wet weight (184-7 + 13-6 mg; < 0-001), but that of the AF rats did not (weight = 122-0 ±4-7 mg). In response to ovariectomy, uteri from both NF and AF rats responded by decreases in wet weight to similar levels (NF = 59-3+1-74; AF = 63-9 + 2-1). Administration of oestradiol increased uterine weight in ovari¬ ectomized rats to levels approaching oestrogen-injected, hemihysterectomized rats (NF = 152-83 ± 7-1 ; AF = 119-3 ± 3-7). These results suggest that the uterus from AF rats is tonically maximally stimulated and is less responsive to oestradiol than the normal uterus, although the response to oestrogen after ovariectomy and uterine atrophy is apparently similar.To test the ability of androgenized rats to respond to oestradiol by the formation of IP, we used the double-labelling method exactly as described by Katzenellenbogen & Gorski (1972). One uterus from a 3-day ovariectomized normal or ovariectomized and androgenized female was incubated in 1 ml Eagle's HeLa tissue culture medium. Control uteri were incubated in the absence of steroid and in the presence of 5/¿Ci L-[4,5-14CJleucine (312 mCi/mmol)/ml while experimental tissues were incubated in medium containing 3-7 IO-8 M-oestradiol-17/? and 20/¿Ci L-[3HJleucine (2-0 Ci/mmol)/ml. Three control and three experimental uteri were

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“…(2007a;2007b) exposed female rats to androgen compounds during intrauterine period at doses of 1.5 mg/kg and 2 mg/d and found a delay in the vaginal opening, agenesis of vaginal orifice and presence of hypospadias. Single dose of 250 µg TP on day of birth was also able to prevent vaginal opening (Wrenn et al, 1969). At low doses of 0.1 and 0.5 mg/d, intrauterineexposed females presented no marked alterations in this parameter (Wolf et al, 2002).…”

Section: Figurementioning

confidence: 84%