Oestrogen secreting Leydig cell tumour and GnRH agonist in‐vivo and in‐vitro studies

Kerlan, V.; Nahoul, K; Abalain, Jean-Hervé; Mangin, P; Bercovici, J. P.

doi:10.1111/j.1365-2265.1992.tb02314.x

Cited by 9 publications

(8 citation statements)

References 10 publications

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“…Leydig cell tumors of the testis have been demonstrated to produce and secrete various sex steroids [1][2][3][4]. In the present case, production and secretion of testosterone and androstenedione were confirmed by increased cencentrations of these hormones both in peripheral and spermatic venous blood samples.…”

Section: Discussionsupporting

confidence: 65%

“…Primary culture of the resected tumors clinically associated with abnormal steroid metabolism can potentially provide important information about neoplastic steroidogenesis including testicular Leydig cell tumor [4]. Primary culture of human steroidogenic tissues or tumors has generally been performed by dispersing the cells and producing a monolayer of cells inculture [9] or short-term incubation of tissue slices [4].…”

Section: Discussionmentioning

confidence: 99%

“…Primary culture of human steroidogenic tissues or tumors has generally been performed by dispersing the cells and producing a monolayer of cells inculture [9] or short-term incubation of tissue slices [4]. These attempts have suffered from difficulty in supporting long term growth of human steroidogenic tumor cells and maintaining their in vivo structural and functional…”

Section: Discussionmentioning

confidence: 99%

“…Key words: Testis, Leydig cell tumor, Histoculture, Testosterone, Immunohistochemistry (Endocrine Journal 43: 73-78, 1996) LEYDIG cell tumors of the testis are rare but the majority are associated with various steroid biosynthetic abnormalities including testosterone and estrogen overproduction [1][2][3][4][5]. Clinical hormonal analysis of testicular Leydig cell tumor has been extensively performed [1][2][3][4][5], but no detailed analysis of steroidogenesis of a Leydig cell tumor itself including in vitro studies has been reported.…”

Section: Introductionmentioning

confidence: 99%

“…Clinical hormonal analysis of testicular Leydig cell tumor has been extensively performed [1][2][3][4][5], but no detailed analysis of steroidogenesis of a Leydig cell tumor itself including in vitro studies has been reported. We had the opportunity to examine an 11-yearold boy with a Leydig cell tumor of the testis with increased plasma androstenedione and testosterone concentrations and pseudoprecocious puberty.…”

Section: Introductionmentioning

confidence: 99%

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Leydig Cell Tumor of the Testis: Analysis of Testosterone Production and Secretion by Three-Dimensional Histoculture.

et al. 1996

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Abstract.We treated an 11-year-old boy with a testicular Leydig cell tumor. We analyzed the testosterone production of this tumor by immunolocalization of steroidogenic enzymes and in vitro three-dimensional histoculture. Spermatic venous blood from the tumor bearing testis had noticeably high concentrations of testosterone and androstenedione. The tumor had the characteristic ultrastructural features of steroid producing cells and was immunoreactive for P450scc (side chain cleavage), 3I3HSD (hydroxysteroid dehydrogenase) and P450c17 (17a-hydroxylase).Three-dimensional collagengel-supported histoculture demonstrated that the tumor tissue in the culture maintained its histologic architecture, expression of steroidogenic enzymes, and secretion of testosterone into the medium for up to 7 days in culture. Histoculture preserved in vitro testosterone production in this case of testicular Leydig cell tumor.Key words: Testis, Leydig cell tumor, Histoculture, Testosterone, Immunohistochemistry (Endocrine Journal 43: 73-78, 1996) LEYDIG cell tumors of the testis are rare but the majority are associated with various steroid biosynthetic abnormalities including testosterone and estrogen overproduction [1][2][3][4][5]. Clinical hormonal analysis of testicular Leydig cell tumor has been extensively performed [1][2][3][4][5], but no detailed analysis of steroidogenesis of a Leydig cell tumor itself including in vitro studies has been reported. We had the opportunity to examine an 11-yearold boy with a Leydig cell tumor of the testis with increased plasma androstenedione and testosterone concentrations and pseudoprecocious puberty. Pathological analysis of the resected specimens including electron microscopy and immuno- The patient was an 11-year-old Japanese boy. His parents noticed linear and skeletal growth acceleration and virilization at the age of 9 years, but he did not receive medical attention at that time. He presented to the pediatrician with complaints of testicular enlargement and premature puberty at the age of 11 years and was referred to Tohoku University Hospital, Sendai, Japan for evaluation of a testicular mass.His height was 160.05 cm and his weight was 58.0 kg on admission, both greater than the 95

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Leydig Cell Tumor of the Testis: Analysis of Testosterone Production and Secretion by Three-Dimensional Histoculture.

et al. 1996

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Leydig Cell Aromatase

Carreau

Contemporary Endocrinology

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Overexpression of Aromatase Leads to Development of Testicular Leydig Cell Tumors

Fowler

Gill

Kirma

et al. 2000

The American Journal of Pathology

113

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Despite recent advances in diagnosis and treatment of testicular cancer, its causes remain unknown. The most common conditions known to be associated with testicular cancer are cryptorchidism, infertility, and overexposure to pesticides or radiation. Recent studies also indicate hormones may play a crucial role in testicular tumorigenesis. Our studies show that about half of the male transgenic mice overexpressing aromatase in testis were infertile and/or had larger than normal testicles. Gross pathology and histological analysis showed the mice to have Leydig cell tumors, unilaterally or bilaterally. Serum estradiol levels for transgenic mice were at least twice as high as those for nontransgenic mice. Expression of aromatase and estrogen receptor were also very high in testicular tissue of transgenic mice compared to nontransgenic mice. Consistent with increased estrogenic activity in the testicular tissue, we also saw an increase in the levels of genes involved in cell cycle that are regulated by the estrogen. To obtain a better understanding of the biological significance of testicular tumorigenesis, a reliable animal model is necessary to clarify the mechanisms and correlations associated with human cancers. Here we describe such a model, which shows that overexpression of aromatase results in increased estrogen production and a changed hormone milieu, leading to the induction of testicular cancer (Leydig cell tumors). This predictable and useful model is a potential tool for the study of testicular tumorigenesis, hormonal carcinogenesis, synergistic action of other carcinogens on hormone-induced tumors, and tumor dependency on endocrine factors.

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Oestrogen secreting Leydig cell tumour and GnRH agonist in‐vivo and in‐vitro studies

Cited by 9 publications

References 10 publications

Leydig Cell Tumor of the Testis: Analysis of Testosterone Production and Secretion by Three-Dimensional Histoculture.

Leydig Cell Tumor of the Testis: Analysis of Testosterone Production and Secretion by Three-Dimensional Histoculture.

Leydig Cell Aromatase

Overexpression of Aromatase Leads to Development of Testicular Leydig Cell Tumors

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