Members of the order Rickettsiales are small, obligate intracellular bacteria that are vector-borne and can cause mild to fatal diseases in humans worldwide. There is little information on the zoonotic rickettsial pathogens that may be harboured by dogs from rural localities in South Africa. To characterize rickettsial pathogens infecting dogs we screened 141 blood samples, 103 ticks and 43 fleas collected from domestic dogs in Bushbuckridge Municipality, Mpumalanga Province of South Africa between October 2011 and May 2012 using the reverse line blot (RLB) and Rickettsia genus and species-specific qPCR assays. Results from RLB showed that 49% of blood samples and 30% of tick pools were positive for the genus-specific probes for Ehrlichia/Anaplasma; 16% of the blood samples were positive for Ehrlichia canis. Haemoparasite DNA could not be detected in 36% of blood samples and 30% of tick pools screened. Seven (70%) of tick pools and both flea pools were positive for Rickettsia spp; three (30%) of tick pools were positive for R. africae and both flea pools phagocytophilum, and an Orientia tsutsugamushi-like sequence were identified from blood samples.The detection of emerging zoonotic agents from domestic dogs and their ectoparasites in a rural community in South Africa highlights the potential risk of human infection that may occur with these pathogens.