Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Guo, Ren; Gao, Peng; Min, Junwei; Zhou, Meiling; Han, Jun; Yu, Xun; Yu, Xianghua; Lei, Ming; Yan, Shaohui; Yang, Yue; Dan, Dan; Ye, Tong

doi:10.1364/ao.52.008233

Cited by 37 publications

(17 citation statements)

References 40 publications

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“…Due to the coherence of the light source, there exist artefacts in intensity images with or without scattering media. Illumination by sources with low coherence length such as LEDs may reduce this type of artefact and improve the imaging quality further [39].…”

Section: Resultsmentioning

confidence: 99%

Rapid wide-field imaging through scattering media by digital holographic wavefront correction

Peng

Zhou

et al. 2019

Appl. Opt.

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Imaging through scattering media has been a long standing challenge in many disciplines. One of the promising solutions to address the challenge is the wavefront shaping technique, in which the phase distortion due to a scattering medium is corrected by a phase modulation device such as a spatial light modulator (SLM). However, the wide-field imaging speed is limited either by the feedback-based optimization to search the correction phase or by the update rate of SLMs. In this report, we introduce a new method called digital holographic wavefront correction, in which the correction phase is determined by a single-shot off-axis holography. The correction phase establishes the so-called “scattering lens”, which allows any objects to be imaged through scattering media; in our case, the “scattering lens” is a digital one established through computational methods. As no SLM is involved in the imaging process, the imaging speed is significantly improved. We have demonstrated that moving objects behind scattering media can be recorded at the speed of 2.8 fps with each frame corrected by the updated correction phase while the image contrast is maintained as high as 0.9. The image speed can potentially reach the video rate if the computing power is sufficiently high. We have also demonstrated that the digital wavefront correction method also works when the light intensity is low, which implicates its potential usefulness in imaging dynamic processes in biological tissues.

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Section: Resultsmentioning

confidence: 99%

Rapid wide-field imaging through scattering media by digital holographic wavefront correction

Peng

Zhou

et al. 2019

Appl. Opt.

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“…The ideal solution, however, would be replacing the coherent laser source with LEDs. Lately, various groups have proposed configurations to achieve off-axis DH microscopy using external optical components to address the low coherence of LEDs 42 , 43 , 44 , 45 . Although effective, these do not fully exploit the advantage of using such a compact light source, as cumbersome interferometric schemes are required and a portable device has not been realized yet.…”

Section: Discussionmentioning

confidence: 99%

Endowing a plain fluidic chip with micro-optics: a holographic microscope slide

et al. 2017

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Lab-on-a-Chip (LoC) devices are extremely promising in that they enable diagnostic functions at the point-of-care. Within this scope, an important goal is to design imaging schemes that can be used out of the laboratory. In this paper, we introduce and test a pocket holographic slide that allows digital holography microscopy to be performed without an interferometer setup. Instead, a commercial off-the-shelf plastic chip is engineered and functionalized with this aim. The microfluidic chip is endowed with micro-optics, that is, a diffraction grating and polymeric lenses, to build an interferometer directly on the chip, avoiding the need for a reference arm and external bulky optical components. Thanks to the single-beam scheme, the system is completely integrated and robust against vibrations, sharing the useful features of any common path interferometer. Hence, it becomes possible to bring holographic functionalities out of the lab, moving complexity from the external optical apparatus to the chip itself. Label-free imaging and quantitative phase contrast mapping of live samples are demonstrated, along with flexible refocusing capabilities. Thus, a liquid volume can be analyzed in one single shot with no need for mechanical scanning systems.

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“…As a typical representative of this technique, digital holographic microscopy (DHM) can be implemented for complex amplitude imaging and be used to investigate transparent specimens, such as biological samples including tissues, dry mass, membrane fluctuation, etc [5][6][7][8]. In DHM, a hologram that carries specimen information is recorded digitally first, and then the hologram is numerically reconstructed to extract the amplitude or phase of the specimens' complex field [9][10][11][12][13]. After that, the quantitative phase information can be converted to dry mass density of the cell with extremely high accuracy which has been demonstrated so far as a valuable tool in hematological or cancer diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Phase Imaging Using Deep Learning-Based Holographic Microscope

Wang

et al. 2021

Front. Phys.

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Digital holographic microscopy enables the measurement of the quantitative light field information and the visualization of transparent specimens. It can be implemented for complex amplitude imaging and thus for the investigation of biological samples including tissues, dry mass, membrane fluctuation, etc. Currently, deep learning technologies are developing rapidly and have already been applied to various important tasks in the coherent imaging. In this paper, an optimized structural convolution neural network PhaseNet is proposed for the reconstruction of digital holograms, and a deep learning-based holographic microscope using above neural network is implemented for quantitative phase imaging. Living mouse osteoblastic cells are quantitatively measured to demonstrate the capability and applicability of the system.

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Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Cited by 37 publications

References 40 publications

Rapid wide-field imaging through scattering media by digital holographic wavefront correction

Rapid wide-field imaging through scattering media by digital holographic wavefront correction

Endowing a plain fluidic chip with micro-optics: a holographic microscope slide

Quantitative Phase Imaging Using Deep Learning-Based Holographic Microscope

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