OFF bipolar cells express distinct types of dendritic glutamate receptors in the mouse retina

Puller, Christian; Ivanova, Elena; Euler, Thomas; Haverkamp, Silke; Schubert, Timm

doi:10.1016/j.neuroscience.2013.03.054

Cited by 58 publications

(87 citation statements)

References 56 publications

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“…Our data, in conjunction with published data on AMPARs, indicate that at least in the adult, KARs are found at OHC afferent synapses but AMPARs are not. The adult pattern of synaptic GluRs, with AMPARs and KARs at IHCs but only KARs at OHCs is consistent with the complex pattern of AMPARs and KARs found in different cell types of the retina (Puller et al, 2013). …”

Section: Discussionsupporting

confidence: 80%

Localization of kainate receptors in inner and outer hair cell synapses

et al. 2014

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Glutamate plays a role in hair cell afferent transmission, but the receptors that mediate neurotransmission between outer hair cells (OHCs) and type II ganglion neurons are not well defined. A previous study using in situ hybridization showed that several kainate-type glutamate receptor (KAR) subunits are expressed in cochlear ganglion neurons. To determine whether KARs are expressed in hair cell synapses, we performed X-gal staining on mice expressing lacZ driven by the GluK5 promoter, and immunolabeling of glutamate receptors in whole-mount mammalian cochleae. X-gal staining revealed GluK5 expression in both type I and type II ganglion neurons and OHCs in adults. OHCs showed X-gal reactivity throughout maturation from postnatal day 4 (P4) to 1.5 months. Immunoreactivity for GluK5 in IHC afferent synapses appeared to be postsynaptic, similar to GluA2 (GluR2; AMPA-type glutamate receptor (AMPAR) subunit), while GluK2 may be on both sides of the synapses. In OHC afferent synapses, immunoreactivity for GluK2 and GluK5 was found, although GluK2 was only in those synapses bearing ribbons. GluA2 was not detected in adult OHC afferent synapses. Interestingly, GluK1, GluK2 and GluK5 were also detected in OHC efferent synapses, forming several active zones in each synaptic area. At P8, GluA2 and all KAR subunits except GluK4 were detected in OHC afferent synapses in the apical turn, and GluA2, GluK1, GluK3 decreased dramatically in the basal turn. These results indicate that AMPARs and KARs (GluK2/GluK5) are localized to IHC afferent synapses, while only KARs (GluK2/GluK5) are localized to OHC afferent synapses in adults. Glutamate spillover near OHCs may act on KARs in OHC efferent terminals to modulate transmission of acoustic information and OHC electromotility.

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Section: Discussionsupporting

confidence: 80%

Localization of kainate receptors in inner and outer hair cell synapses

et al. 2014

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“…Their synapses are so-called ‘sign-preserving’ because like photoreceptors, horizontal cells and OFF-bipolar cells are depolarized at light offset. Mouse OFF-bipolar cell dendrites express AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) and/or Kainate receptors and different proportions of AMPA (GluA1 subunit) and Kainate (GluK1 subunit) receptors have been localized on dendrites of distinct OFF-bipolar cell subtypes (Puller et al, 2013). However, recent imaging of glutamate release from OFF-bipolar cells in mouse retina and electrophysiological recordings from Type 4 OFF-bipolar cells have proposed that Kainate receptors are instrumental in mediating light-evoked responses of mouse OFF-bipolar cells (Borghuis et al, 2014).…”

Section: Synapse Structure and Connectivity Of Retinal Neuronsmentioning

confidence: 99%

Functional architecture of the retina: Development and disease

Hoon

Okawa

Santina

et al. 2014

Progress in Retinal and Eye Research

467

385

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Structure and function are highly correlated in the vertebrate retina, a sensory tissue that is organized into cell layers with microcircuits working in parallel and together to encode visual information. All vertebrate retinas share a fundamental plan, comprising five major neuronal cell classes with cell body distributions and connectivity arranged in stereotypic patterns. Conserved features in retinal design have enabled detailed analysis and comparisons of structure, connectivity and function across species. Each species, however, can adopt structural and/or functional retinal specializations, implementing variations to the basic design in order to satisfy unique requirements in visual function. Recent advances in molecular tools, imaging and electrophysiological approaches have greatly facilitated identification of the cellular and molecular mechanisms that establish the fundamental organization of the retina and the specializations of its microcircuits during development. Here, we review advances in our understanding of how these mechanisms act to shape structure and function at the single cell level, to coordinate the assembly of cell populations, and to define their specific circuitry. We also highlight how structure is rearranged and function is disrupted in disease, and discuss current approaches to re-establish the intricate functional architecture of the retina.

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“…The ON bipolar cells express the metabotropic glutamate receptor 6 (mGluR6) and the OFF bipolar cells express different variations of ionotropic glutamate receptors (iGluR) of AMPA and kainate types (Slaughter and Miller, 1981;Puller et al, 2013). A decrease in glutamate release at the photoreceptor terminals cause the OFF BCs to hyperpolarize due to closure of the iGluR cation channel .…”

Section: On Bipolar Cell Signalingmentioning

confidence: 99%

Constructing the rod bipolar cell signalplex using animal models of retinal dysfunction.

Ray¹,

Andrew²

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OFF bipolar cells express distinct types of dendritic glutamate receptors in the mouse retina

Cited by 58 publications

References 56 publications

Localization of kainate receptors in inner and outer hair cell synapses

Localization of kainate receptors in inner and outer hair cell synapses

Functional architecture of the retina: Development and disease

Constructing the rod bipolar cell signalplex using animal models of retinal dysfunction.

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