Off-season flowering and expression of flowering-related genes during floral bud differentiation of rabbiteye blueberry in a subtropical climate

Omori, Masami; Cheng, Chieh-Chi; Hsu, Fu-Chiun; Chen, Shiang-Jiuun; Yamane, Hisayo; Tao, Ryutaro; Li, Kuo‐Tan

doi:10.1016/j.scienta.2022.111458

Cited by 6 publications

(7 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression studies have brought to light varying facets: grape CO linked to flowering initiation and COL1 associated with dormancy ( Almada et al., 2009 ); six pear ( Pyrus bretschneideri ) COLs , out of a total of 15, showed circadian clock and photoperiod-regulated expression ( Wang et al., 2017 ); and Mango ( Mangifera indica L.) CO and COLs implicated in photoperiod-mediated flowering, with CO-FT conservativity warranting further clarification ( Liu et al., 2020a ; Liu et al., 2022 ). Similar investigations have extended to bamboo ( Phyllostachys violascens ), poplar ( Populus trichocarpa ), and Rabbiteye blueberries ( Vaccinium virgatum Aiton), demonstrating their roles in photoperiod-controlled flowering through expression patterns or Arabidopsis ectopic expression ( Xiao et al., 2018 ; Li et al., 2020 ; Omori et al., 2022 ).…”

Section: Major Flowering Pathway Genes In Deciduous Woody Plantsmentioning

confidence: 67%

“…The promotion of flowering by AP1 in various woody plants supports these findings (see Table 1 ). Counter to the flowering promotion led by VcFT-CX, the functional opposite, VcTFL1 , triggers flowering delay ( Omori et al., 2020 ; Omori et al., 2021 ; Omori et al., 2022 ). Intriguingly, VcFT-CX resulted in a reduction in VcTFL1 expression within young leaves ( Walworth et al., 2016 ).…”

Section: Ft -Dominated Floral Induction and Soc1 ...mentioning

confidence: 99%

See 1 more Smart Citation

Regulatory frameworks involved in the floral induction, formation and developmental programming of woody horticultural plants: a case study on blueberries

Song,

Liu,

Zhong

2024

Front. Plant Sci.

View full text Add to dashboard Cite

Flowering represents a crucial stage in the life cycles of plants. Ensuring strong and consistent flowering is vital for maintaining crop production amidst the challenges presented by climate change. In this review, we summarized key recent efforts aimed at unraveling the complexities of plant flowering through genetic, genomic, physiological, and biochemical studies in woody species, with a special focus on the genetic control of floral initiation and activation in woody horticultural species. Key topics covered in the review include major flowering pathway genes in deciduous woody plants, regulation of the phase transition from juvenile to adult stage, the roles of CONSTANS (CO) and CO-like gene and FLOWERING LOCUS T genes in flower induction, the floral regulatory role of GA-DELLA pathway, and the multifunctional roles of MADS-box genes in flowering and dormancy release triggered by chilling. Based on our own research work in blueberries, we highlighted the central roles played by two key flowering pathway genes, FLOWERING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1, which regulate floral initiation and activation (dormancy release), respectively. Collectively, our survey shows both the conserved and diverse aspects of the flowering pathway in annual and woody plants, providing insights into the potential molecular mechanisms governing woody plants. This paves the way for enhancing the resilience and productivity of fruit-bearing crops in the face of changing climatic conditions, all through the perspective of genetic interventions.

show abstract

Section: Major Flowering Pathway Genes In Deciduous Woody Plantsmentioning

confidence: 67%

Section: Ft -Dominated Floral Induction and Soc1 ...mentioning

confidence: 99%

Regulatory frameworks involved in the floral induction, formation and developmental programming of woody horticultural plants: a case study on blueberries

Song,

Liu,

Zhong

2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…However, with the exception of blueberry, it has not been reported that mature flower buds in those precociously flowering woody plants still require chilling to bloom ( Walworth et al, 2016 ). Recent genetic studies have demonstrated that VcFT , VcCOL5 (blueberry CONSTANS-LIKE 5), and VcTFL1 are major flowering regulators in blueberry ( Gaire and Wilde, 2018 ; Omori et al, 2020 ; Omori et al, 2022 ). Increased expression of VcFT with decreased VcCOL5 is responsible for the off-season apical flowering of Rabbiteye blueberries ( Vaccinium virgatum Aiton) ( Omori et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…Recent genetic studies have demonstrated that VcFT , VcCOL5 (blueberry CONSTANS-LIKE 5), and VcTFL1 are major flowering regulators in blueberry ( Gaire and Wilde, 2018 ; Omori et al, 2020 ; Omori et al, 2022 ). Increased expression of VcFT with decreased VcCOL5 is responsible for the off-season apical flowering of Rabbiteye blueberries ( Vaccinium virgatum Aiton) ( Omori et al, 2022 ). A question remains as to why mature flower buds in FT - OX woody plants had a chilling requirement to break their endodormancy.…”

Section: Discussionmentioning

confidence: 99%

Multiple transcriptome comparisons reveal the essential roles of FLOWERING LOCUS T in floral initiation and SOC1 and SVP in floral activation in blueberry

2023

View full text Add to dashboard Cite

The flowering mechanisms, especially chilling requirement-regulated flowering, in deciduous woody crops remain to be elucidated. Flower buds of northern highbush blueberry cultivar Aurora require approximately 1,000 chilling hours to bloom. Overexpression of a blueberry FLOWERING LOCUS T (VcFT) enabled precocious flowering of transgenic “Aurora” mainly in non-terminated apical buds during flower bud formation, meanwhile, most of the mature flower buds could not break until they received enough chilling hours. In this study, we highlighted two groups of differentially expressed genes (DEGs) in flower buds caused by VcFT overexpression (VcFT-OX) and full chilling. We compared the two groups of DEGs with a focus on flowering pathway genes. We found: 1) In non-chilled flower buds, VcFT-OX drove a high VcFT expression and repressed expression of a major MADS-box gene, blueberry SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (VcSOC1) resulting an increased VcFT/VcSOC1 expression ratio; 2) In fully chilled flower buds that are ready to break, the chilling upregulated VcSOC1 expression in non-transgenic “Aurora” and repressed VcFT expression in VcFT-OX “Aurora”, and each resulted in a decreased ratio of VcFT to VcSOC1; additionally, expression of a blueberry SHORT VEGETATIVE PHASE (VcSVP) was upregulated in chilled flower buds of both transgenic and non-transgenic’ “Aurora”. Together with additional analysis of VcFT and VcSOC1 in the transcriptome data of other genotypes and tissues, we provide evidence to support that VcFT expression plays a significant role in promoting floral initiation and that VcSOC1 expression is a key floral activator. We thus propose a new hypothesis on blueberry flowering mechanism, of which the ratios of VcFT-to-VcSOC1 at transcript levels in the flowering pathways determine flower bud formation and bud breaking. Generally, an increased VcFT/VcSOC1 ratio or increased VcSOC1 in leaf promotes precocious flowering and flower bud formation, and a decreased VcFT/VcSOC1 ratio with increased VcSOC1 in fully chilled flower buds contributes to flower bud breaking.

show abstract

“…In addition, blueberry is a chilling-mediated flowering species that requires a certain number of chilling hours before flowering; fully chilled blueberry plants flower normally, whereas non-chilled plants might not flower [ 24 ]. Under natural conditions, most blueberry cultivars initiate flower buds in autumn and bloom the following spring [ 25 ]. In Shanghai, China, the blueberry buds begin to differentiate in July as soon as the harvest is finished, under a SD length rhythm.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-Based Identification of Candidate Flowering-Associated Genes of Blueberry in a Plant Factory with Artificial Lighting (PFAL) under Short-Day-Length Conditions

An,

Zhang,

et al. 2024

IJMS

View full text Add to dashboard Cite

In blueberry (Vaccinium corymbosum L.), a perennial shrub, flower bud initiation is mediated by a short-day (SD) photoperiod and buds bloom once the chilling requirement is satisfied. A plant factory with artificial lighting (PFAL) is a planting system that can provide a stable and highly efficient growing environment for blueberry production. However, the characteristics of bud differentiation of blueberry plants inside PFAL systems are poorly understood. To better understand flower bud initiation and the flowering mechanism of blueberry in PFAL systems, the anatomical structure of apical buds under SD conditions in a PFAL system was observed using the southern highbush cultivar ‘Misty’ and a transcriptomic analysis was performed to identify the candidate flowering genes. The results indicated that the apical bud of ‘Misty’ differentiated gradually along with SD time course and swelled obviously when chilling was introduced. A total of 39.28 Gb clean data were generated, and about 20.31–24.11 Mb high-quality clean reads were assembled, yielding a total of 17370 differentially expressed genes (DEGs), of which 9637 were up-regulated and 7733 were down-regulated. Based on the functional annotation, 26 DEGs were identified including 20 flowering-related and 6 low-temperature DEGs, out of which the expressive level of four flowering-related DEGs (VcFT2, VcFPA, VcFMADS1, and VcCOP1) and two low-temperature-induced DEGs (VcTIL-1 and VcLTI 65-like) were confirmed by qRT-PCR with a good consistency with the pattern of transcriptome. Functional analysis indicated that VcFT2 was highly conserved with nuclear and cytoplasmic subcellular localization and was expressed mainly in blueberry leaf tissue. In Arabidopsis, ectopic overexpression of VcFT2 results in an early flowering phenotype, indicating that VcFT2 is a vital regulator of the SD-mediated flowering pathway in blueberry. These results contribute to the investigation of photoperiod-mediated flowering mechanisms of blueberry in PFAL systems.

show abstract

Off-season flowering and expression of flowering-related genes during floral bud differentiation of rabbiteye blueberry in a subtropical climate

Cited by 6 publications

References 16 publications

Regulatory frameworks involved in the floral induction, formation and developmental programming of woody horticultural plants: a case study on blueberries

Regulatory frameworks involved in the floral induction, formation and developmental programming of woody horticultural plants: a case study on blueberries

Multiple transcriptome comparisons reveal the essential roles of FLOWERING LOCUS T in floral initiation and SOC1 and SVP in floral activation in blueberry

Transcriptome-Based Identification of Candidate Flowering-Associated Genes of Blueberry in a Plant Factory with Artificial Lighting (PFAL) under Short-Day-Length Conditions

Contact Info

Product

Resources

About