Off-target effects of an insect cell-expressed influenza HA-pseudotyped Gag-VLP preparation in limiting postinfluenza Staphylococcus aureus infections

Klausberger, Miriam; Ленева, И. А.; Egorov, Andrey I.; Strobl, Florian; Ghorbanpour, Sahar Masoumeh; Фалынскова, И. Н.; Поддубиков, А. В.; Махмудова, Н. Р.; Krokhin, Artem; Svitich, Oxana; Grabherr, Reingard

doi:10.1016/j.vaccine.2019.10.083

Cited by 13 publications

(14 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recombinant baculoviruses for the expression of HA-Gag and Gag only -VLPs were generated as described in [22]. Briefly, the HA of A/Puerto Rico/8/1934 was expressed under control of the AcMNPV p10 promoter, whereas the HIV-1 Gag protein was under control of the AcMNPV pH promoter.…”

Section: Production and Purification Of Influenza Ha-gag Vlpsmentioning

confidence: 99%

“…Dialyzed VLP suspensions were either treated with 5 mM binary ethylenimine (BEI) (Sigma, St. Louis, MO) for 36 h or with 16 mM β-propiolactone (βPL) (Acros Organics, Geel, BE) for 16 h at 37 • C in a water bath. A 0.1 M BEI solution was prepared, as previously described [22]. After inactivation, residual BEI or βPL in the preparation was inactivated by 15 mM or 160 mM sodium thiosulfate (Na 2 S 2 O 3 , final concentration), respectively, and preparations were again dialyzed for 19 h against 500 dialysis volumes of 20 mM HEPES pH 7.4.…”

Section: Production and Purification Of Influenza Ha-gag Vlpsmentioning

confidence: 99%

“…HA content. The HA content of the HA-Gag VLP preparations was determined using the Influenza A H1N1 (A/Puerto Rico/8/1934) Hemagglutinin/HA ELISA pair set (Sino Biological, Wayne, PA, USA) with some deviations from the manufacturer's protocol, as previously described [22]. Briefly, we employed a recombinant soluble, trimeric insect cell-expressed PR8 HA protein as calibration standard [26] and pre-treated samples with Zwittergent 3-14 for the solubilization of VLPs.…”

Section: Characterization Of Ha-gag Vlpsmentioning

confidence: 99%

“…With the widespread introduction of childhood pneumococcal immunization programs vaccine serotypes in circulation have been rapidly replaced by non-vaccine serotypes, which compromises the benefit of implemented programs [18]. There is a limited but growing number of studies available that acknowledge the protective role of influenza vaccination in the context of secondary bacterial infections (SBIs) in the mouse model and in humans [19][20][21][22]. Influenza vaccination predominantly focuses on the induction of antibodies towards the head domain of the influenza hemagglutinin (HA).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Potential of Influenza HA-Specific Immunity in Mitigating Lethality of Postinfluenza Pneumococcal Infections

et al. 2019

View full text Add to dashboard Cite

: Influenza virus infections pre-dispose an individual to secondary pneumococcal infections, which represent a serious public health concern. Matching influenza vaccination was demonstrated helpful in preventing postinfluenza bacterial infections and associated illnesses in humans. Yet, the impact of influenza hemagglutinin (HA)-specific immunity alone in this dual-infection scenario remains elusive. In the present study, we assessed the protective effect of neutralizing and non-neutralizing anti-hemagglutinin immunity in a BALB/c influenza-pneumococcus superinfection model. Our immunogens were insect cell-expressed hemagglutinin-Gag virus-like particles that had been differentially-treated for the inactivation of bioprocess-related baculovirus impurities. We evaluated the potential of several formulations to restrain the primary infection with vaccine-matched or -mismatched influenza strains and secondary bacterial replication. In addition, we investigated the effect of anti-HA immunity on the interferon status in mouse lungs prior to bacterial challenge. In our experimental setup, neutralizing anti-HA immunity provided significant but incomplete protection from postinfluenza bacterial superinfection, despite effective control of viral replication. In view of this, it was surprising to observe a survival advantage with non-neutralizing adaptive immunity when using a heterologous viral challenge strain. Our findings suggest that both neutralizing and non-neutralizing anti-HA immunity can reduce disease and mortality caused by postinfluenza pneumococcal infections.

show abstract

Section: Production and Purification Of Influenza Ha-gag Vlpsmentioning

confidence: 99%

Section: Production and Purification Of Influenza Ha-gag Vlpsmentioning

confidence: 99%

Section: Characterization Of Ha-gag Vlpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Potential of Influenza HA-Specific Immunity in Mitigating Lethality of Postinfluenza Pneumococcal Infections

et al. 2019

View full text Add to dashboard Cite

show abstract

“…В случае несовпадения вакцинного штамма с циркулирующим наблюдается отсутствие адекватной защиты от первичной инфекции, что может приводить к незащищенности вакцинированных от бактериальных осложнений [17]. Нами ранее на экспериментальной модели вторичной бактериальной пневмонии мышей, индуцированной S. pneumoniae или S. aureus после гриппозной инфекции, показано, что вакцинация вирусоподобными частицами (ВПЧ), несущими НА ВГ, обеспечивает защиту от бактериальных суперинфекций после гриппозной инфекции, инициированной гомологичным, но не гетерологичным ВГ [21,22].…”

Section: Introductionunclassified

The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice

Ленева¹,

Фалынскова²,

Карташова³

et al. 2021

Journal of microbiology, epidemiology and immunobiology

View full text Add to dashboard Cite

Introduction. Pneumonia often occurs secondary to influenza infection and accounts for a large proportion of the morbidity and mortality associated with seasonal and pandemic influenza outbreaks. We previously have shown that vaccination with Virus-like particles (VLPs) containing hemagglutinin (HA) of influenza virus reduces mortality caused by bacterial infections after an influenza infections in mice.The aim of this work is to study whether this protective effect may be potentiated by supplementing the HA preparation with the influenza neuraminidase (NA).Materials and methods. We studied the effect of Gag-VLPs with the influenza HA or NA from А/PR/8/34 alone or in combination, in a lethal BALB/c mouse model of S. aureus infection after vaccine-matched or mismatched influenza virus challenge.Results. A cocktail of HA-Gag and NA-Gag-VLPs fully protected from weight loss, mortality and viral replication and significantly reduced the bacterial burden in the lungs of А/PR/8/34 infected animals. Immunization with this cocktail HA-Gag-VLPs 100 ng + NA-Gag-VLPs 20 ng also protected 60% of animals from mortality associated with secondary bacterial S. aureus infection following a heterologous H1N1 influenza virus challenge, and led to the significant protection from weight loss and pulmonary pathogen replication even in the absence of HA-inhibition and NA-inhibition antibodies.Conclusion. Our results indicate that influenza vaccination may improve the outcome of a secondary bacterial pneumonia induced by S. aureus after influenza even when the virus is antigenically different from the vaccine strain. At the same time, in our model, the significance of the immunity to influenza virus HA was prevalent.

show abstract

Virusähnliche Partikel – Impfstoffe, die den Eindringling imitieren

Klausberger,

Satzer,

Aguilar

2024

Biospektrum

View full text Add to dashboard Cite

show abstract

Off-target effects of an insect cell-expressed influenza HA-pseudotyped Gag-VLP preparation in limiting postinfluenza Staphylococcus aureus infections

Cited by 13 publications

References 39 publications

The Potential of Influenza HA-Specific Immunity in Mitigating Lethality of Postinfluenza Pneumococcal Infections

The Potential of Influenza HA-Specific Immunity in Mitigating Lethality of Postinfluenza Pneumococcal Infections

The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice

Virusähnliche Partikel – Impfstoffe, die den Eindringling imitieren

Contact Info

Product

Resources

About