Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization

Luo, Huacheng; Li, Xiaoyong; Tian, Geng; Li, Dali; Hou, Changliang; Ding, Xinbao; Hou, Lin; Lyu, Qifeng; Yang, Yuanyuan; Cooney, Austin J.; Xie, Wenhai; Xiong, Ji; Wang, Hu; Zhao, Xiaodong; Wu, Ji

doi:10.1016/j.jare.2021.03.006

Cited by 25 publications

(23 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Stella -overexpressing FGSC lines were generated as previously described 9 . Briefly, gene coding sequences were amplified and cloned into the multiple cloning site of lentivirus overexpression vectors.…”

Section: Methodsmentioning

confidence: 99%

“…In situ Hi-C was performed as previously reported 9 , 32 . Briefly, cells were fixed, lysed, and digested with Dpn II restriction enzyme.…”

Section: Methodsmentioning

confidence: 99%

“…Ma et al revealed that the PI3K-AKT pathway is important for FGSC maintenance 5 . Etv5 , Foxo1 , and Akt were also shown to positively regulate FGSC self-renewal 9 . In addition, Zou et al evaluated different differentiation conditions of mouse FGSCs in vitro , and successfully differentiated FGSCs into GV oocytes 8 .…”

Section: Introductionmentioning

confidence: 95%

“…In addition to its role in mouse oocytes and embryos, Stella also affects the differentiation of human embryonic stem cells 22 and tumor cells 23 . Recent studies in our laboratory have shown that, after overexpressing Stella , H19 , and Zfp57, and knockdown of Plzf in spermatogonial stem cells (SSCs), SSCs could be converted into induction of germline stem cells, which had similar morphology, DNA methylation pattern, and 3D chromatin structure as FGSCs 9 . This finding suggests Stella is an important maternal-origin gene.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Stella Regulates the Development of Female Germline Stem Cells by Modulating Chromatin Structure and DNA Methylation

Hou¹,

Zhao²,

Tian³

et al. 2022

Int. J. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

Female germline stem cells (FGSCs) have the ability to self-renew and differentiate into oocytes. Stella , encoded by a maternal effect gene, plays an important role in oogenesis and early embryonic development. However, its function in FGSCs remains unclear. In this study, we showed that CRISPR/Cas9-mediated knockout of Stella promoted FGSC proliferation and reduced the level of genome-wide DNA methylation of FGSCs. Conversely, Stella overexpression led to the opposite results, and enhanced FGSC differentiation. We also performed an integrative analysis of chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), high-throughput genome-wide chromosome conformation capture (Hi-C), and use of our published epigenetic data. Results indicated that the binding sites of STELLA and active histones H3K4me3 and H3K27ac were enriched near the TAD boundaries. Hi-C analysis showed that Stella overexpression attenuated the interaction within TADs, and interestingly enhanced the TAD boundary strength in STELLA-associated regions. Taking these findings together, our study not only reveals the role of Stella in regulating DNA methylation and chromatin structure, but also provides a better understanding of FGSC development.

show abstract

Section: Methodsmentioning

confidence: 99%

“…In situ Hi-C was performed as previously reported 9 , 32 . Briefly, cells were fixed, lysed, and digested with Dpn II restriction enzyme.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stella Regulates the Development of Female Germline Stem Cells by Modulating Chromatin Structure and DNA Methylation

Hou¹,

Zhao²,

Tian³

et al. 2022

Int. J. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yamashiro, C. et al took a major step forward successfully in human vitro gametogenesis by coculturing hPGCLCs with mouse embryonic ovarian somatic cells to generate xenogeneic reconstituted ovaries (xrOvaries), a type of organoid, which were cultured in vitro for 4 months to produce oogonia ( Yamashiro et al, 2018 ). The entry of hPGCLCs into gametogenesis in vitro is possible due to the successes of in vitro gametogenesis in mice by the coculture of mPGCLCs with mouse embryonic somatic gonadal cells in vitro or their transplantation into the corresponding position in vivo ( Hayashi et al, 2011 ; Hayashi et al, 2012 ; Hikabe et al, 2016 ; Ishikura et al, 2016 ; Luo et al, 2021 ). However, human embryonic somatic gonadal cells cannot easily obtain due to ethical and technical difficulties, which also prevents the verification of the ability of hPGCLCs to enter the later stage of germ cell development.…”

Section: In Vitro Induced Gametes Using Pluripotent Stem Cellsmentioning

confidence: 99%

Research Advances in Gametogenesis and Embryogenesis Using Pluripotent Stem Cells

Luo

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The previous studies of human gametogenesis and embryogenesis have left many unanswered questions, which hinders the understanding of the physiology of these two vital processes and the development of diagnosis and treatment strategies for related diseases. Although many results have been obtained from animal studies, particularly mouse research, the results cannot be fully applied to humans due to species differences in physiology and pathology. However, due to ethical and material limitations, the direct study of human gametes and embryos is very difficult. The emergence and rapid development of organoids allow the construction of organoid systems that simulate gametogenesis and embryogenesis in vitro, and many studies have successfully established organoid systems for some parts of or even the entire processes of gametogenesis and embryogenesis. These studies typically start with the establishment of mouse models and then modify these models to obtain human organoid models. These organoid models can be used to obtain a better understanding of the signaling pathways, molecular mechanisms, genetics, and epigenetic changes involved in gametogenesis and embryogenesis and could also be applied to clinical applications, such as drug screening. Here, we discuss the formation of primordial stem cell-like cells (PGCLCs), and in vitro-induced gametes and embryoids using pluripotent stem cells (PSCs). We also analyze their applications and limitations.

show abstract

Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair

Mu,

Li,

Yang

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Ovarian dysfunction is a major factor leading to female infertility. Understanding how to improve or reshape ovarian function has become an important entry point for preventing and treating female infertility caused by ovarian dysfunction. Here, plant‐derived compounds are screened for in vitro activity upon ovarian organoids derived from feeder‐free female germline stem cells. Tetrahydroxy stilbene glucoside (TSG) is found to promote the development of ovarian organoids. Single nucleus transcriptome sequencing and spatial transcriptome sequencing are used to establish a comprehensive spatiotemporal map to elucidate the role of TSG in ovarian organoid development, encompassing cell types and subtypes, transcription factors, pseudo‐time sequence, and cell communication dynamics. This analysis indicates that TSG promotes ovarian organoid development through the vascular endothelial growth factor A‐Eph receptor B2 ligand‐receptor pair between granulosa cells and oocytes. This study has enhanced the understanding of the mechanisms of ovarian organoid development, establishes a technical platform for screening compounds for treating infertility and related diseases, and lays a foundation for clinically applying plant‐derived compounds.

show abstract

Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization

Cited by 25 publications

References 64 publications

Stella Regulates the Development of Female Germline Stem Cells by Modulating Chromatin Structure and DNA Methylation

Stella Regulates the Development of Female Germline Stem Cells by Modulating Chromatin Structure and DNA Methylation

Research Advances in Gametogenesis and Embryogenesis Using Pluripotent Stem Cells

Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa‐Ephb2 Pair

Contact Info

Product

Resources

About