Offspring resulting from transfer of cryopreserved embryos in camel (Camelus dromedarius)

Nowshari, M.A.; Ali, Syed Azmal; Saleem, Shazia

doi:10.1016/j.theriogenology.2004.10.014

Cited by 20 publications

(10 citation statements)

References 31 publications

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“…In vivo-produced embryos of dromedary camel (Camelus dromedarius), collected at the blastocyst stage, were vitrified. Post-warming survival and intact morphology were high (92%) and following transfer of 45 embryos (20 during the breeding season and 25 off-season), three pregnancy were obtained, one of which was carried to term (Nowshari et al, 2005). This report follows a previous one in which cryopreserved embryos did not lead to a pregnancy after transfer (Skidmore & Loskutoff, 1999).…”

Section: Ungulatessupporting

confidence: 67%

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Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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Section: Ungulatessupporting

confidence: 67%

“…Freezingpregnancy by ultrasound, vitrificationpregnancy to term (Skidmore & Loskutoff, 1999;Nowshari et al, 2005) www. (Swanson, 2001(Swanson, , 2003 Tigrina (Leopardus tigrinus)…”

Section: Domestic Donkey (Equus Acinus)mentioning

confidence: 99%

Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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“…However, in our studies, the beneficial effect has been observed only when sucrose is added to the cryopreservation media but when cryoprotectant removal is performed in MEM without sucrose. Nowshari et al (2005) and Bianchi et al (2005) have obtained good results with cryopreserved camel embryos and human oocytes, respectively, when cryoprotectant removal is carried out in medium without sucrose. Martinez-Madrid et al (2004) have also obtained encouraging results after cryoprotectant removal from frozen/thawed human ovaries by a series of decreasing concentrations of sucrose (0.25 M, 0.1 M, and 0 M).…”

Section: Discussionmentioning

confidence: 99%

Preservation of caprine preantral follicle viability after cryopreservation in sucrose and ethylene glycol

et al. 2006

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Caprine preantral follicles within ovarian fragments were cryopreserved in the absence or presence of 0.5 M sucrose with or without 1 M dimethyl sulfoxide and/or 1 M ethylene glycol (EG). After being thawed, they were washed in minimum essential medium with or without 0.3 M sucrose. Histological analysis of follicle integrity immediately after cryopreservation showed consistent beneficial effects of including sucrose in the three cryoprotectant solutions analyzed when tissue was thawed without sucrose (53.9+/-14.8-82.4+/-3.2% normal vs 27.6+/-1.6-36.6+/-6.5%, P<0.05). However, in further studies, the addition of sucrose to the thaw solutions proved detrimental or of no benefit. An analysis of the cryopreserved material with calcein-AM and ethidium homodimer (markers for living and dead cells, respectively) gave comparable results to those obtained by histology. Follicles cryopreserved in EG, EG plus sucrose, or sucrose alone were cultured in vitro for 24 h following warming. During this culture period, viability fell most rapidly in material cryopreserved in sucrose alone and was no longer correlated with either the viability or integrity estimates made immediately after warming. By contrast, the viability of follicles cryopreserved in EG with sucrose and then cultured for 24 h was not significantly different from the cultured non-frozen controls. These results indicate that cryopreservation in 1 M EG plus 0.5 M sucrose combined with thawing without sucrose is effective for caprine ovarian tissue.

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“…In camelids, although EG was successfully used in vitro vitrification of dromedary (Nowshari, Sayed, & Saleem, ), llama (Aller et al, ; Palasz, Adams, Brogliatti, & Mapletoft, ) and alpaca (Vivanco‐Mackie, ) embryos as well as alpaca oocytes (Ruiz et al, ) , there were no available data about its role in vitrification of dromedary oocytes. From the present study, it was found that vitrification using 40% EG was a better cryoprotectant compared to DMSO as shown by high acceptable viable rate of C. dromedaries oocytes (86.11 ± 5.6).…”

Section: Discussionmentioning

confidence: 99%

Effects of cryoprotectants and cryoprotectant combinations on viability and maturation rates of Camelus dromedarius oocytes vitrified at germinal vesicle stage

Moawad

Hussein

El-Ghani

et al. 2018

Reprod Domestic Animals

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Camel fertility faces many problems, which could be solved by assisted reproductive technologies (ARTs). We designed the experiment to explore the effect of different cryoprotectant concentrations and combinations on viability and maturation rates of vitrified/warmed camel oocytes. We collected ovaries directly after slaughtering from local abattoir and transported them to laboratory in a thermo-flask containing normal physiological saline. We aspirated the oocytes from follicles, which is 2-8 mm in diameter, washed three times in TCM-199 and then examined under stereo-microscope for selection. We selected morphologically normal oocytes with an evenly granulated cytoplasm and a compact cumulus cell layer. We equilibrated morphologically normal oocytes in equilibration solution (ES), which is half concentration of vitrification one. After equilibration, We transported oocytes to vitrification solution using ethylene glycol (EG, 40%), dimethyl sulphoxide (DMSO, 40%) and EG 40% + DMSO 40%. The obtained results revealed that addition of EG 40% + DMSO 40% resulted in the best quality of vitrified/warmed oocytes, which is demonstrated by higher per cent survival rate (90.16%) and maturation rate (58.95%). While DMSO 40% resulted in 62.79% and 29.54%, respectively, EG 40% reported 86.11% and 53.47%, respectively. We could conclude that vitrification of immature camel oocytes by using 40% EG + 40% DMSO is suitable methods to limit drawbacks of vitrification methods, and we need further studies to assess the ability of in vitro-produced blastocyst to develop in vivo and establish pregnancy after embryo transfer.

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Offspring resulting from transfer of cryopreserved embryos in camel (Camelus dromedarius)

Cited by 20 publications

References 31 publications

Genome Banking for Vertebrates Wildlife Conservation

Genome Banking for Vertebrates Wildlife Conservation

Preservation of caprine preantral follicle viability after cryopreservation in sucrose and ethylene glycol

Effects of cryoprotectants and cryoprotectant combinations on viability and maturation rates of Camelus dromedarius oocytes vitrified at germinal vesicle stage

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