Ofloxacin-Loaded PLLA Nanofibrous Mats for Wound Dressing Applications

Abstract

Poly­(l-lactide) (PLLA) was synthesized from l-lactide in the presence of a zinc salen complex. Ofloxacin (OFLX)-loaded PLLA nanofibrous mats were fabricated by electrospinning using dichloromethane/dimethyl sulfoxide (4:1 v/v) solutions containing different amounts of OFLX (1, 3, 5, and 10 w/w%). The morphology and diameter of nanofibrous mats were studied by scanning electron microscopy (SEM). The internal morphology of the nanofibers was examined by transmission electron microscopy (TEM). Miscibility between OFLX and PLLA in nanofibrous mats was confirmed by attenuated total reflection–Fourier transform infrared (ATR–FTIR) spectroscopy. Differential scanning calorimetry (DSC) confirmed the interaction of OFLX with PLLA nanofibrous mats. The presence of OFLX in PLLA nanofibrous mats increased the tensile strength significantly, which was confirmed by dynamic mechanical analysis (DMA). The in vitro drug release profile was studied at pH values 4.6, 5.8, and 7.4. OFLX-loaded PLLA nanofibrous mats showed burst release till first 12 h, and sustained release followed up to 168 h. The in vitro biocompatibility test of nanofibrous mats was carried out using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against the NIH/3T3 fibroblast cell line. The results of MTT assay demonstrated cell viability and cell proliferation at a concentration as low as 10 w/w%, which proved their nontoxicity and biocompatibility. OFLX-loaded PLLA nanofibrous mats exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli. The in vivo wound healing study on rats showed 44, 65, and 88% wound closure rates on 14th day with control, PLLA, and OFLX-loaded PLLA nanofibrous mats, respectively. The results proved that the PLLA nanofibrous mats loaded with an appropriate concentration of OFLX are promising candidates for wound dressing applications.

Nanofibers Embedded with Nanoparticles as Carriers for the Controlled Release of Anticancer Drug: Promoting the Apoptosis of Breast Cancer Cell Line and Growth Inhibition of Microbial Strains

“…The pure PLA showed a characteristic peak at 1746.17 cm –1 that indicates the carbonyl group of ester and, furthermore, the stretching vibrations of C–H was appeared at 2997.75 and 2944.61 cm –1 . Meanwhile, the other characteristic peak observed at 1182.52 cm –1 was assigned to the C–O–C stretching vibration of the ether group. , All the nanofiber mats confirmed the presence of the ester group (−CO) at 1756.08, 1755.92, 1755.9, and 1754.95 cm –1 shifting from 1746.17 cm –1 from pure PLA. The −NH stretching vibration of new peaks were assigned for pristine NF, Fe 3 O 4 –NF1, Fe 3 O 4 –NF2, and Fe 3 O 4 –NF3 at 3131.78, 3146.14, 3160.51, and 3153.32 cm –1 , respectively, in addition to the amide bands appeared at 1671.92, 1668.35, 1670.47, and 1669.47 cm –1 for the nanofiber mats. , …”

“…The pure PLA showed a characteristic peak at 1746.17 cm −1 that indicates the carbonyl group of ester and, furthermore, the stretching vibrations of C−H was appeared at 2997.75 and 2944.61 cm −1 . Meanwhile, the other characteristic peak observed at 1182.52 cm −1 was assigned to the C−O−C stretching vibration of the ether group 20,21. All the nanofiber mats confirmed the presence of the ester group (−C�O) at 1756.08, 1755.92, 1755.9, and 1754.95 cm −1 shifting from 1746.17 cm −1 from pure PLA.…”

Antibacterial Porous Coaxial Drug-Carrying Nanofibers for Sustained Drug-Releasing Applications

“…PPR1 also showed double melting peaks at 144.88 and 152.36 °C that were related to the two different crystalline morphologies of PLA. There might be hydrogen bonding among ROX, PLA, and PCL, which reduced the thermal stability of the drug-loaded nanofibers [ 29 ]. In Figure 5 b, as shown in P1, PCL had good stability, with an extrapolated onset decomposing temperature (T e ) of 380.14 °C.…”

Advancements in release‐active antimicrobial biomaterials: A journey from release to relief

“…Ofloxacin, gentamycin, or vancomycin were loaded into the poly(L-lactide) nanofiber-based wound healing mat scaffold to tackle infection of acute and chronic wounds. Moreover, encapsulation of the growth factors in this mat also helped in chronic wound healing (Rade & Garnaik, 2020). Ampicillin and cefepime were loaded in polyacrylic acid and graphene-oxide crosslinked mesh, which exhibited no-inflammatory response at S. aureus infected superficial wound (Altinbasak et al, 2018).…”