Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities

Miller, John I.; Techtmann, Stephen M.; Fortney, Julian L.; Mahmoudi, Nagissa; Joyner, Dominique C.; Liu, Jiang; Olesen, Scott W.; Alm, Eric J.; Maza, Adolfo; Gardinali, Piero R.; GaraJayeva, Nargiz; Askerov, Faig S.; Hazen, Terry C.

doi:10.3389/fmicb.2019.00995

Cited by 32 publications

(15 citation statements)

References 67 publications

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“…7 ), consistent with the significantly higher losses of PAHs from these treatments compared to diesel amendments ( Fig. 2C and D ) and with previous reports of aromatic hydrocarbon biodegradation by Amphritea ( 50 – 52 ). MAGs with novel classifications in the Genome Taxonomy Database (GTDB) exhibiting hydrocarbon biodegradation potential included Sphingorhabdus_ A, 50-400-T64, Pseudomonas _ D, and PGZG01 ( Fig.…”

Section: Resultssupporting

confidence: 91%

Diesel and Crude Oil Biodegradation by Cold-Adapted Microbial Communities in the Labrador Sea

Murphy

Bautista

Cramm

et al. 2021

Appl Environ Microbiol

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Oil spills in the subarctic marine environment off the coast of Labrador, Canada, are increasingly likely due to potential oil production and increases in ship traffic in the region. To understand the microbiome response and how nutrient biostimulation promotes biodegradation of oil spills in this cold marine setting, marine sediment microcosms amended with diesel or crude oil were incubated at in situ temperature (4°C) for several weeks. Sequencing of 16S rRNA genes following these spill simulations revealed decreased microbial diversity and enrichment of putative hydrocarbonoclastic bacteria that differed by petroleum product. Metagenomic sequencing revealed Paraperlucidibaca and Cycloclasticus harbour previously unrecognized capabilities for alkane biodegradation. Genomic and amplicon sequencing together suggest that Oleispira and Thalassolituus degraded alkanes from diesel, while Zhongshania and the novel PGZG01 lineage contributed to crude oil alkane biodegradation. Greater losses in PAHs from crude oil than from diesel were consistent with Marinobacter , Pseudomonas _ D and Amphritea genomes exhibiting aromatic hydrocarbon biodegradation potential. Biostimulation with nitrogen and phosphorus (4.67 mM NH 4 Cl; 1.47 mM KH 2 PO 4 ) was effective at enhancing n -alkane and PAH degradation following low concentration (0.1% v/v) diesel and crude oil amendments, while at higher concentrations (1% v/v) only n -alkanes in diesel were consumed, suggesting toxicity induced by compounds in unrefined crude oil. Biostimulation allowed for a more rapid turnover in the microbial community in response to petroleum amendments, more than doubling the rates of CO 2 increase during the first few weeks of incubation. Importance Increases in transportation of diesel and crude oil in the Labrador Sea will pose a significant threat to remote benthic and shoreline environments, where coastal communities and wildlife are particularly vulnerable to oil spill contaminants. Whereas marine microbiology has not been incorporated into environmental assessments in the Labrador Sea, there is a growing demand for microbial biodiversity evaluations given the pronounced impact of climate change in this region. Benthic microbial communities are important to consider given that a fraction of spilled oil typically sinks such that its biodegradation occurs at the seafloor, where novel taxa with previously unrecognized potential to degrade hydrocarbons were discovered in this work. Understanding how cold-adapted microbiomes catalyze hydrocarbon degradation at low in situ temperature is crucial in the Labrador Sea, which remains relatively cold throughout the year.

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Section: Resultssupporting

confidence: 91%

Diesel and Crude Oil Biodegradation by Cold-Adapted Microbial Communities in the Labrador Sea

Murphy

Bautista

Cramm

et al. 2021

Appl Environ Microbiol

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“…However, after the 15th subculture, they still grew in all consortia, except in C4. Those Gammaproteobacteria were regularly discovered in the oil-impacted marine environments and were found competent in degrading oil [39]. They were also explored for their ability to degrade low molecular weight alkanes, aromatics, BTEX (benzene, toluene, ethylbenzene and xylenes), proline, catechol, cyclohexanone, and nitroalkanes.…”

Section: Discussionmentioning

confidence: 99%

Potential of Biosurfactants’ Production on Degrading Heavy Oil by Bacterial Consortia Obtained from Tsunami-Induced Oil-Spilled Beach Areas in Miyagi, Japan

Primeia

Inoue

Chien

2020

JMSE

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Bioremediation is one of the promising environment-friendly approaches to eliminate oil contamination. However, heavy oil is known to degrade slowly due to its hydrophobicity. Therefore, microorganisms capable of producing biosurfactants are gaining substantial interest because of their potential to alter hydrocarbon properties and thereby speed up the degradation process. In this study, six bacterial consortia were obtained from the oil-spilled beach areas in Miyagi, Japan, and all of which exhibited high potential in degrading heavy oil measured by gas chromatography with flame ionization detector (GC-FID). The polymerase chain reaction—denaturing gradient gel electrophoresis (PCR-DGGE) and next-generation sequencing (NGS) revealed that the diverse microbial community in each consortium changed with subculture and became stable with a few effective microorganisms after 15 generations. The total petroleum hydrocarbons (TPH) degradation ability of the consortia obtained from a former gas station (C1: 81%) and oil refinery company (C6: 79%) was higher than that of the consortia obtained from wastewater treatment plant (WWTP) (C3: 67%, and C5: 73%), indicating that bacteria present in C1 and C6 were historically exposed to petroleum hydrocarbons. Moreover, it was intriguing that the consortium C4, also obtained from WWTP, exhibited high TPH degradation ability (77%). The NGS results revealed that two bacteria, Achromobacter sp. and Ochrobactrum sp., occupied more than 99% of the consortium C4, while no Pseudomonas sp. was found in C4, though this bacterium was observed in other consortia and is also known to be a potential candidate for TPH degradation as reported by previous studies. In addition, the consortium C4 showed high biosurfactant-producing ability among the studied consortia. To date, no study has reported the TPH degradation by the combination of Achromobacter sp. and Ochrobactrum sp.; therefore, the consortium C4 provided an excellent opportunity to study the interaction of and biosurfactant production by these two bacteria during TPH degradation.

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“…A microcosm study [ 81 ] reported better degradation of 19 and 21C aliphatic chains under anoxic than oxic conditions. In the same study, heavy aliphatic hydrocarbons (>28C) were completely degraded in oxic microcosms but not in anoxic.…”

Section: Metabolic Mechanisms Of Bacterial Biodegradation Of Hydromentioning

confidence: 99%

“…In anoxic microcosms, most but not all the EPA16 compounds were degraded, with benz(a)anthracene and chrysene being particularly effectively degraded. A higher proportion of dibenzothiophene was degraded in anoxic than oxic microcosms [ 81 ].…”

Section: Metabolic Mechanisms Of Bacterial Biodegradation Of Hydromentioning

confidence: 99%

Oil Bioremediation in the Marine Environment of Antarctica: A Review and Bibliometric Keyword Cluster Analysis

et al. 2021

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Bioremediation of hydrocarbons has received much attention in recent decades, particularly relating to fuel and other oils. While of great relevance globally, there has recently been increasing interest in hydrocarbon bioremediation in the marine environments of Antarctica. To provide an objective assessment of the research interest in this field we used VOSviewer software to analyze publication data obtained from the ScienceDirect database covering the period 1970 to the present, but with a primary focus on the years 2000–2020. A bibliometric analysis of the database allowed identification of the co-occurrence of keywords. There was an increasing trend over time for publications relating to oil bioremediation in maritime Antarctica, including both studies on marine bioremediation and of the metabolic pathways of hydrocarbon degradation. Studies of marine anaerobic degradation remain under-represented compared to those of aerobic degradation. Emerging keywords in recent years included bioprospecting, metagenomic, bioindicator, and giving insight into changing research foci, such as increasing attention to microbial diversity. The study of microbial genomes using metagenomic approaches or whole genome studies is increasing rapidly and is likely to drive emerging fields in future, including rapid expansion of bioprospecting in diverse fields of biotechnology.

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Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities

Cited by 32 publications

References 67 publications

Diesel and Crude Oil Biodegradation by Cold-Adapted Microbial Communities in the Labrador Sea

Diesel and Crude Oil Biodegradation by Cold-Adapted Microbial Communities in the Labrador Sea

Potential of Biosurfactants’ Production on Degrading Heavy Oil by Bacterial Consortia Obtained from Tsunami-Induced Oil-Spilled Beach Areas in Miyagi, Japan

Oil Bioremediation in the Marine Environment of Antarctica: A Review and Bibliometric Keyword Cluster Analysis

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