Oil in water nanoemulsion formulations of botanical active substances

Pascual‐Villalobos, M. J.; Guirao, P.; Baños, F. Guillermo Díaz; Cantó‐Tejero, Manuel; Víllora, Gloria

doi:10.1016/b978-0-12-815829-6.00009-7

Cited by 39 publications

(37 citation statements)

References 15 publications

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“…As defined by Nikam et al [ 39 ], NEs are kinetically stable “biphasic dispersions of two immiscible liquids: either water-in-oil (W/O) or oil-in-water (O/W) droplets stabilized by an amphiphilic surfactant”; in this way, protection from the surrounding environment, suitable spreading, and penetration of the bioactive molecules are guaranteed by the matrix and low surface and interfacial tension [ 40 ]. Toxicity of EO-based NEs was tested on several insects of agricultural and medical interest such as aphids [ 41 , 42 , 43 ], mosquitoes [ 44 , 45 , 46 ], stored-product beetles [ 47 , 48 ], and some Lepidoptera [ 49 , 50 , 51 ]. Furthermore, it was also highlighted that the bioactivity of EO-based NEs was often higher compared to the EOs themselves [ 52 , 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

et al. 2020

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The growing interest in the development of green pest management strategies is leading to the exploitation of essential oils (EOs) as promising botanical pesticides. In this respect, nanotechnology could efficiently support the use of EOs through their encapsulation into stable nanoformulations, such as nanoemulsions (NEs), to improve their stability and efficacy. This technology assures the improvement of the chemical stability, hydrophilicity, and environmental persistence of EOs, giving an added value for the fabrication of natural insecticides effective against a wide spectrum of insect vectors and pests of public and agronomical importance. Carlina acaulis (Asteraceae) root EO has been recently proposed as a promising ingredient of a new generation of botanical insecticides. In the present study, a highly stable C. acaulis-based NE was developed. Interestingly, such a nanosystem was able to encapsulate 6% (w/w) of C. acaulis EO, showing a mean diameter of around 140 nm and a SOR (surfactant-to-oil ratio) of 0.6. Its stability was evaluated in a storage period of six months and corroborated by an accelerated stability study. Therefore, the C. acaulis EO and C. acaulis-based NE were evaluated for their toxicity against 1st instar larvae of the European grapevine moth (EGVM), Lobesia botrana (Denis & Schiffermüller, 1775) (Lepidoptera: Tortricidae), a major vineyard pest. The chemical composition of C. acaulis EO was investigated by gas chromatography–mass spectrometry (GC–MS) revealing carlina oxide, a polyacetylene, as the main constituent. In toxicity assays, both the C. acaulis EO and the C. acaulis-based NE were highly toxic to L. botrana larvae, with LC50 values of 7.299 and 9.044 µL/mL for C. acaulis EO and NE, respectively. The C. acaulis-based NE represents a promising option to develop highly stable botanical insecticides for pest management. To date, this study represents the first evidence about the insecticidal toxicity of EOs and EO-based NEs against this major grapevine pest.

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Section: Introductionmentioning

confidence: 99%

Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

et al. 2020

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“…This is due to the fact that an emulsion consists of lipid droplets dispersed in an aqueous medium and this system is difficult to stabilize due to the large number of complex microstructures. 125,126 Considering the demand for novel natural products to diminish the use of emulsifiers derived from genetically modified plants, such as soy, which can alter the natural flavor and texture of foods, biosurfactants can be used to replace these compounds and enhance these factors. 127,128 Although biosurfactants have explicit advantages over their counterparts, there are no reports of their use as emulsifying agents.…”

Section: Applications Of Biosurfactant In the Food Industrymentioning

confidence: 99%

“…As emulsions naturally form from the different combinations of these ingredients, these additives are essential to the adequate performance of the end product. This is due to the fact that an emulsion consists of lipid droplets dispersed in an aqueous medium and this system is difficult to stabilize due to the large number of complex microstructures 125,126 …”

Section: Biosurfactantsmentioning

confidence: 99%

Biosurfactants: Production and application prospects in the food industry

Ribeiro

Guerra

Sarubbo

2020

Biotechnology Progress

108

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There has been considerable interest in the use of biosurfactants due to the diversity of structures and the possibility of production from a variety of substrates. The potential for industrial applications has been growing, as these natural compounds are tolerant to common processing methods and can compete with synthetic surfactants with regards to the capacity to reduce surface and interfacial tensions as well as stabilise emulsions while offering the advantages of biodegradability and low toxicity. Among biosurfactant-producing microorganisms, some yeasts present no risks of toxicity or pathogenicity, making them ideal for use in food formulations. Indeed, the use of these biomolecules in foods has attracted industrial interest due to their properties as emulsifiers and stabilizers of emulsions. Studies have also demonstrated other valuable properties, such as antioxidant and antimicrobial activity, enabling the aggregation of greater value to products and the avoidance of contamination both during and after processing. All these characteristics allow biosurfactants to be used as additives and versatile ingredients for the processing of foods. The present review discusses the potential application of biosurfactants as emulsifying agents in food formulations, such as salad dressing, bread, cakes, cookies, and ice cream. The antioxidant, antimicrobial and anti-adhesive properties of these biomolecules are also discussed, demonstrating the need for further studies to make the use of the natural compounds viable in this expanding sector.

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“…Other references in the literature [10][11][12] point out at direct contact activity of the substances against aphids and stored products pests (Ephestia kuehniella Zeller, Lepidoptera: Pyralidae). Fennel EO (with 67.5% of (E)-anethole and 25.5% of fenchone) was more active in M. persicae than anise (93% (E)-anethole), contact LD 50 = 0.06 or 0.43%, respectively, by spraying on aphid-infested cabbage plants [13].…”

Section: Introductionmentioning

confidence: 99%

Fumigant Toxicity in Myzus persicae Sulzer (Hemiptera: Aphididae): Controlled Release of (E)-anethole from Microspheres

Pascual‐Villalobos

Cantó‐Tejero

Guirao

et al. 2020

Plants

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(E)-anethole is a phenylpropanoid that is the main compound found in the essential oils (EOs) of anise and fennel seeds, and either fumigant or direct contact activity of this compound has been demonstrated against aphids and stored product pests. In this work, solid microspheres were prepared by three methods—oil emulsion entrapment, spray-drying, and complexed with β-cyclodextrin. Fumigation activity of each microsphere preparation was tested against the green peach aphid, Myzus persicae Sulzer (Hemiptera: Aphididae), on pepper leaves. The best insecticidal activity was with (E)-anethole encapsulated in oil emulsion beads and introduced to aphids as a vapour over 24 h, with an LC50 of 0.415 μL/L compared to 0.336 μL/L of vapors from free (E)-anethole. Scanning electron microscopy of the beads revealed a compact surface with low porosity that produced a controlled release of the bioactive for more than 21 d, whilst most of the volatile was evaporated within two days if applied unformulated. Spray drying gave spherical particles with the greatest encapsulated yield (73%) of 6.15 g of (E)-anethole incorporated per 100 g of powder. Further work will be done on improving the formulation methods and testing the solid microspheres in all aphid stages scaling up the experimental assay. It is foreseen that nanotechnology will play a role in future developments of low risk plant protection products.

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Oil in water nanoemulsion formulations of botanical active substances

Cited by 39 publications

References 15 publications

Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

Developing a Highly Stable Carlina acaulis Essential Oil Nanoemulsion for Managing Lobesia botrana

Biosurfactants: Production and application prospects in the food industry

Fumigant Toxicity in Myzus persicae Sulzer (Hemiptera: Aphididae): Controlled Release of (E)-anethole from Microspheres

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