Oil palm drought inducible DREB1 induced expression of DRE/CRT- and non-DRE/CRT-containing genes in lowland transgenic tomato under cold and PEG treatments

Azzeme, Azzreena Mohamad; Abdullah, Siti Nor Akmar; Aziz, Maheran Abdul; Wahab, Puteri Edaroyati Megat

doi:10.1016/j.plaphy.2016.12.025

Cited by 27 publications

(24 citation statements)

References 60 publications

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“…MT1) seeds were purchased from the Malaysian Agricultural Research and Development Institute (MARDI), Serdang and kept at 4 °C. The seeds were further germinated on Murashigae and Skoog (MS) media as described by Azzeme et al (2017).…”

Section: Plants and Agrobacterium-mediated Transformationmentioning

confidence: 99%

“…Two hundred of 7-to 10-d-old cotyledons were cut and co-cultivated with Agrobacterium tumefaciens strain LBA4404 harboring pMDC-EgDREB1 and pMDC(-32) (empty vector) as described by Azzeme et al (2017). The vector map of pMDC-EgDREB1 and pMDC(-32) is described in Fig.…”

Section: Plants and Agrobacterium-mediated Transformationmentioning

confidence: 99%

“…Six independent transgenic lines harboring Elaeis guineensis DREB1 (EgDREB1) (named as EgDREB1-L5, EgDREB1-L9, EgDREB1-L13, EgDREB1-L1, EgDREB1-L3, and EgDREB1-L10), transgenic plant harboring empty vector [named as pMDC(-32)] and WT plant were grown in the greenhouse, Universiti Putra Malaysia, at natural conditions (a temperature 28 -30 °C) (Azzeme et al 2017). The empty vector plant harbouring only a pMDC-32 vector and not the ccdB and EgDREB1 genes.…”

Section: Verification and Regeneration Of Transgenic Plantsmentioning

confidence: 99%

“…Involvement of DREB1 gene in controlling SRG expression has been widely reported. The gene has the ability to enhance stress tolerance to drought, freezing and salinity based on the reports from various plants including tomato (Azzeme et al 2017), wheat (Wang et al 2006), rice (Ito et al 2006), potato (Bouaziz et al 2012), and barley (Soltesz et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…EgDREB1 in PEGand cold-treated lowland tomato seedlings upregulated the expression of tomato genes like peroxidase, ascorbate peroxidase, glutathione peroxidase, catalase, heat shock protein 70, late embryogenesis abundant, metallothionein type 2, delta 1-pyrroline-5-carboxylate synthetase and adenylate isopentenyltransferase. These genes contain DRE/CRT motif in their promoter sequence (Azzeme et al 2017). Overexpression of citrus DREB in tomato, however, produced smaller fruit size and induced accumulation of primary metabolites (Nishawy et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of oil palm EgDREB1 in tomato decreased fruit sizeand produced parthenocarpic fruits

Azzeme¹,

Abdullah²,

Aziz³

et al. 2020

Biol. plant.

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Drought-responsive element binding (DREB) is involved in the regulation of stress-responsive gene expressions in plants through abscisic acid (ABA)-independent pathway. In this study, constitutive expression of oil palm (Elaeis guineensis) EgDREB1 driven by double strength cauliflower mosaic virus 35S promoter in tomato (Solanum lycopersicum) reduced seed number, produced parthenocarpic fruits, changed morphology of leaves, and increased root biomass of transgenic plants. Early flowering and fruiting of the transgenic lines were observed in the culture vessels. EgDREB1 was specifically expressed in the fruits and its expression was not detected in vegetative tissues (leaves and roots). Altered expression of several endogenous tomato genes involved in the biosynthesis of phytohormones including jasmonic acid, ethylene, auxin, cytokinin, gibberellin (GA) and ABA were observed compared to wild type plants. The expression of AP2-likeethylene transcription factor (LeAP2), allene oxide synthase (LeAOS), allene oxide cyclase (LeAOC), aminocyclopropane-1-carboxylic acid synthase (LeACS), 1-aminocyclopropane-1-carboxylate oxidase 1 (LeACO), auxin responsive factor 8 (LeARF8), auxin/indole-3-acetic acid (LeAux/IAA), cytokinin oxidase/dehydrogenase-like (LeSlCKX1), adenylate isopentenyltransferase (LeSlIPT1), gibberellin 2-oxidase 2 (LeGa2ox2), gibberellin 20-oxidase 4 (LeGa20ox4) and ABAaldehyde oxidase (LeAAO) were different in fruits with reduced seed number compared to parthenocarphic fruits. These results suggest that their expression has significant effects on fruit development in transgenic tomato. EgDREB1 may mediate the expression of some of these genes as dehydration-responsive element binding (DRE) motif were found in their promoter sequences. These data indicate that the EgDREB1 controls fruit development in trsngenic plants by regulating the expression of hormone-associated genes.

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Section: Plants and Agrobacterium-mediated Transformationmentioning

confidence: 99%

Section: Plants and Agrobacterium-mediated Transformationmentioning

confidence: 99%

Section: Verification and Regeneration Of Transgenic Plantsmentioning

confidence: 99%