Ointment ofBrassica oleraceavar.capitataMatures the Extracellular Matrix in Skin Wounds of Wistar Rats

Abstract

Wound healing is a complex process that aims to restore damaged tissue. Phytotherapeutics, such as cabbage, Brassica oleracea var. capitata (Brassicaceae), and sunflower, Helianthus annuus L. (Asteraceae) oil, are used as wound healers. Five circular wounds, each 12 mm in diameter, were made in the dorsolateral region of each rat. The animals were divided into four groups: balsam (B. oleracea); ointment (B. oleracea); sunflower oil (Helianthus annuus); control (saline solution 0.9%). These products were applied daily for 20 days and every four days the tissues of different wounds were removed. The wound contraction area, total collagen, types I and III collagen, glycosaminoglycans, and tissue cellularity were analyzed. In the groups that received ointment and balsam there was reduction in the wound area on days 4, 8, 12, and 20. Throughout the trial period, the balsam and ointment groups showed a higher amount of total collagen, type I collagen, and glycosaminoglycan compared to the others groups. The rats in the groups treated with B. oleracea var. capitata showed a higher number of cells on days 8, 16, and 20. B. oleracea was effective in stimulating the maturation of collagen and increasing the cellularity, as also in improving the mechanical resistance of the newly formed tissue.

Wound-Healing potential of Sebastiania hispida (Mart.) Pax (Euphorbiaceae) ointment compared to low power laser in rats

“…By week 2, type I collagen again becomes the principal collagen produced by fibroblasts. Type I and type III collagen are formed in skin in a higher proportion in relation to other types and are maintained in a fixed relative proportion to one another in normal skin tissue (Cheng et al, 2011;Sarandy et al, 2015).…”

Effect of Topical Administration of Fractions and Isolated Molecules from Plant Extracts on Skin Wound Healing: A Systematic Review of Murine Experimental Models

“…The use of plant based strategies is opening a new perspective for the treatment of skin wounds, mainly in developing countries, once it represents a simple, low cost, and affordable therapy [1, 7, 56–58]. There are several studies indicating beneficial effects of herbal medicines in all phases of the healing process.…”

“…These parameters indicate if the wound closure follows a normal process, in which the newly formed tissue gradually develops drastic structural changes to reconstitute the morphofunctional characteristics of the intact skin. Works which demonstrated the importance of these analyses assert that the type and quantity of collagen fibers deposited on the tissue can be used as a marker of tissue mechanical resistance [2, 3, 9, 58]. The connective and epithelial tissues form a support structure to promote the correct closure of the wound [57, 63], reducing the chances of opportunistic infections in the wounded area [38, 64].…”

Ethno-Herbal-Medico in Wound Repair: An Incisive Review

“…Moreover, wound healing agent showed the efficient functioning via the Wnt/β-catenin pathway. Sarandy et al (2015) evaluated the reduction of the wound area, reconstruction of extracellular matrix and cell proliferation of the cutaneous scar tissue of Wistar rats treated with ointment Brassica oleracea var. capitata extract.…”