Okült Hepati̇t B Vi̇rüsünün Genoti̇p, Subgenoti̇p Ve Subti̇p Anali̇zi̇

Çakal, Bülent; Atasoy, Alp; Çavuş, Bilger; Örmeci, Aslı; Poda, Mehveş; Bulakçı, Mesut; Güllüoğlu, Mine; Günver, Mehmet Güven; Akyüz, Filiz

doi:10.26650/iuitfd.2020.0093

Cited by 1 publication

(2 citation statements)

References 14 publications

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“…sequences in the database. Based on BLAST aligment analysis, our LAMP primer region exhibits similarities of 93% for genotypes A (A1-A2-A3), 92% for genotype B, 95% for genotype C, 99% for genotype D, 94% for genotype E, 91% for genotype F, 92% for genotype G, and 93% for genotype H. Our primer region was found to be 99% similar to genotype D, which is the most common genotype in Türkiye (8).…”

Section: Accepted Manuscriptmentioning

confidence: 79%

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Development of Molecular-Based Screening Test for Hepatitis B Virus in Human Plasma Samples

Altın,

Agel

2024

Jpn J Infect Dis

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Despite the HBV vaccine being routinely administered in many countries, the death rate remains significant. Antiviral medications are available for the treatment of HBV infection, but patients encounter various serious complications in cases of chronic HBV infection. The failure of serological tests to detect early viral replication prevents early treatment response. Recently, many studies have demonstrated significant advantages of the loop-mediated isothermal amplification (LAMP) assay over serological testing and PCR for the rapid detection ofA c c e p t e d M a n u s c r i p tmicrobial pathogens. This study developed a rapid, sensitive, and portable system-integrative LAMP assay to detect Hepatitis B DNA in plasma samples. The final optimized assay was achieved with an amplification time of less than 45 minutes at 62°C. As a result of testing 77 HBV-positive plasma samples with known Cq values, the LAMP assay showed 100% specificity, 92, 20% sensitivity, and a detection limit of 10 copies/µL. Our results showed that the colorimetric LAMP assay is a sensitive, efficient, and highly reliable assay for the rapid detection of HBV and has the potential to be used as a screening test in areas where poor laboratory facilities and limited resources are available.

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Section: Accepted Manuscriptmentioning

confidence: 79%

“…indicates that genotype D is dominant in this area (8,9,10). With a 99% similarity rate, our LAMP primer is capable of identifying the most common genotype, genotype D, in Türkiye.…”

Section: Türkiye Is a Region With Intermediate Endemicity Of Hbv Infe...mentioning

confidence: 97%

Development of Molecular-Based Screening Test for Hepatitis B Virus in Human Plasma Samples

Altın,

Agel

2024

Jpn J Infect Dis

View full text Add to dashboard Cite

show abstract

Okült Hepati̇t B Vi̇rüsünün Genoti̇p, Subgenoti̇p Ve Subti̇p Anali̇zi̇

Cited by 1 publication

References 14 publications

Development of Molecular-Based Screening Test for Hepatitis B Virus in Human Plasma Samples

Development of Molecular-Based Screening Test for Hepatitis B Virus in Human Plasma Samples

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