Olanzapine: evaluation of the <i>in vivo</i> cytogenetic effect

Constantinos, Zampas; Papadopoulou, E.; Goulas, A.; Iakovidou‐Kritsi, Zafiroula

doi:10.1002/hup.2471

Cited by 3 publications

(9 citation statements)

References 10 publications

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“…OLP, an antagonist of dopamine [36], was used to verify whether NONHSAT089447 expression was induced by dopamine. OLP was added at varying concentrations to cells pre-treated with dopamine.…”

Section: Discussionmentioning

confidence: 99%

Regulatory Role of lncRNA NONHSAT089447 in the Dopamine Signaling Pathway in Schizophrenic Patients

Chen¹,

Zhu²,

Niu³

et al. 2019

Med Sci Monit

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Background We previously discovered that 3 long non-coding RNAs (lncRNAs) NONHSAT089447, NONHSAT021545, and NONHSAT041499 were differentially expressed in the peripheral blood of patients with schizophrenia, in comparison to those in normal healthy controls. In this study, we conducted bioinformatic analysis of these 3 lncRNAs and the regulatory role of lncRNA NONHSAT089447 in the dopamine signaling pathway in patients with schizophrenia. Material/Methods There lncRNAs in peripheral blood mononuclear cells (PBMCs) were screened using microarray analysis. Pearson’s correlation analysis was performed to assess the levels of co-expressed mRNAs of respective lncRNAs. The Database for Annotation, Visualization and Integrated Discovery (DAVID) software was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes or Genomes (KEGG) enrichment analysis for these lncRNAs. Human neuroblastoma cell lines (SK-N-SH) were cultured and treated with dopamine or olanzapine (OLP), or transfected with siRNA targeting NONHSAT089447 or plasmid expressing NONHSAT089447. Levels of lncRNAs were detected by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). Then, mRNA and protein expression of the dopamine receptors DRD1, DRD2, DRD3, DRD4, and DRD5 were measured by RT-PCR and western blot analysis, respectively. Results OLP treatment significantly inhibited the expression of NONHSAT089447. Knockdown of NONHSAT089447 by siRNA decreased DRD3 and DRD5 expression, while overexpression of NONHSAT089447 significantly upregulated expression of DRD3 and DRD5. Western blot analysis confirmed that levels of NONHSAT089447 regulated downstream DRD signaling. Conclusions Our results revealed that the lncRNA NONHSAT089447 participated in the dopamine signaling pathway via upregulation of DRDs.

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Section: Discussionmentioning

confidence: 99%

Regulatory Role of lncRNA NONHSAT089447 in the Dopamine Signaling Pathway in Schizophrenic Patients

Chen¹,

Zhu²,

Niu³

et al. 2019

Med Sci Monit

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“…Olanzapine, an atypical antipsychotic drug, is approved for treating schizophrenia, bipolar disorder, and other psychiatric disorders. It is commonly used off-label for treating other conditions, such as eating disorders [ 1 ]. It is an antagonist of the D2 dopamine and 5-HT2A serotonin receptors and has antagonist properties at M1-muscarinic, H1-histaminic, and alpha-1 adrenergic receptors [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is commonly used off-label for treating other conditions, such as eating disorders [ 1 ]. It is an antagonist of the D2 dopamine and 5-HT2A serotonin receptors and has antagonist properties at M1-muscarinic, H1-histaminic, and alpha-1 adrenergic receptors [ 1 ]. Olanzapine, like other atypical antipsychotics, displays serious adverse events in patients’ metabolic profiles like weight gain and insulin resistance [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

“…Olanzapine’s cytogenetic behavior has been studied in vitro in lymphocytes from healthy individuals [ 7 , 8 ], and in vivo in lymphocytes from schizophrenia patients [ 1 ]. Olanzapine in vitro seems to have cytotoxic but not cytostatic effects on cultured lymphocytes of healthy donors [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Olanzapine in vitro seems to have cytotoxic but not cytostatic effects on cultured lymphocytes of healthy donors [ 7 ]. Olanzapine appears cytotoxic but not cytostatic in schizophrenic patients, but the danger of cytotoxicity seems to be more prominent with known DNA-damaging agents such as smoking [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

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Olanzapine’s Cytogenetic Effect on T Lymphocytes in Systemic Lupus Erythematosus and Rheumatoid Arthritis Patients: In Vitro Study

Demirtzoglou¹,

Chrysoglou²,

Katopodi³

et al. 2023

Cureus

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Objectives: This study will investigate olanzapine’s cytogenetic behavior in cultured human T lymphocytes in patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Methods: Three olanzapine solutions were added in cultures of peripheral blood lymphocytes of healthy individuals, SLE, and RA patients. After 72 hours of incubation, the cultured lymphocytes were plated on glass slides and stained with the fluorescence plus Giemsa method. Sister chromatid exchanges (SCEs), proliferation rate index (PRI), and mitotic index (MI) were measured with the optical microscope. Results: There was a statistically significant (p=0.001) dose-dependent increase of SCEs in SLE and RA patients compared to healthy individuals and a statistically significant (p=0.001) reduction of PRI and MI in the highest concentration in the SLE group. Moreover, Spearman's rank correlation coefficient was applied to calculate the correlation between SCEs, PRI, and MI. Negative significant correlations were noticed for both patient groups concerning SCEs-PRI alterations and SCEs-MI alterations. Conversely, positive correlations were noticed for both patient groups for PRI-MI alterations. Conclusions: Olanzapine affects T lymphocytes from SLE and RA patients by modifying DNA replication procedures and DNA damage response. Considering the use of olanzapine in neuropsychiatric symptoms of SLE, further in vivo studies are necessary to evaluate its effect on human DNA.

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Antipsychotics influence Toll-like receptor (TLR) expression and its relationship with cognitive functions in schizophrenia

Kéri

Szabó

Kelemen

2017

Brain, Behavior, and Immunity

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Olanzapine: evaluation of the in vivo cytogenetic effect

Abstract: Our results indicate a mild cytotoxic-but not cytostatic-effect of OLZ which was more prominent in smokers and in chronically treated patients. That effect should be taken into consideration by psychiatrists upon assessing the benefit/risk ratio of their prescriptions.

Cited by 3 publications

References 10 publications

Regulatory Role of lncRNA NONHSAT089447 in the Dopamine Signaling Pathway in Schizophrenic Patients

Regulatory Role of lncRNA NONHSAT089447 in the Dopamine Signaling Pathway in Schizophrenic Patients

Olanzapine’s Cytogenetic Effect on T Lymphocytes in Systemic Lupus Erythematosus and Rheumatoid Arthritis Patients: In Vitro Study

Antipsychotics influence Toll-like receptor (TLR) expression and its relationship with cognitive functions in schizophrenia

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