2020
DOI: 10.1177/0963689720947098
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“Old School” Islet Purification Based on the Unit Gravity Sedimentation as a Rescue Technique for Intraportal Islet Transplantation—A Case Report

Abstract: Here, we present a case that required a supplemental “old school” islet purification for a safe intraportal infusion. Following pancreas procurement from a brain-dead 26-year-old male donor (body mass index: 21.9), 24.6 ml of islet tissue was isolated after continuous density gradient centrifugation. The islet yield was 504,000 islet equivalent (IEQ), distributed among the following three fractions: 64,161 IEQ in 0.6 ml of pellet, 182,058 IEQ in 10 ml, and 258,010 IEQ in 14 ml with 95%, 20%, and 10% purity, re… Show more

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“… 21 There are additional options available for pellet volume reduction such as the unit gravity sedimentation-based “old school” supplementation islet purification technique, which requires minimal islet manipulation. 22 The drawback to purification is the potential to lose viable islet cells, resulting in poorer islet recovery for transplant. The decision to use purification is dependent on the postdigestion tissue volume and was performed in 15 of 20 patients in the remote group for tissue volumes >15 mL.…”
Section: Discussionmentioning
confidence: 99%
“… 21 There are additional options available for pellet volume reduction such as the unit gravity sedimentation-based “old school” supplementation islet purification technique, which requires minimal islet manipulation. 22 The drawback to purification is the potential to lose viable islet cells, resulting in poorer islet recovery for transplant. The decision to use purification is dependent on the postdigestion tissue volume and was performed in 15 of 20 patients in the remote group for tissue volumes >15 mL.…”
Section: Discussionmentioning
confidence: 99%