Old World and Clade C New World Arenaviruses Mimic the Molecular Mechanism of Receptor Recognition Used by α-Dystroglycan's Host-Derived Ligands

Rojek, Jillian M.; Spiropoulou, Christina F.; Campbell, Kevin P.; Kunz, Stefan

doi:10.1128/jvi.02574-06

Cited by 68 publications

(86 citation statements)

References 51 publications

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“…The cells were then chilled on ice and detached with enzyme-free cell dissociation, and the cell surface expression of ␣-DG and TfR1 was assessed by flow cytometry (9,20).…”

Section: Methodsmentioning

confidence: 99%

“…High Throughput Screening (HTS) of Combinatorial Chemical Libraries-Retroviral pseudotypes of LASV, JUNV, GTOV, and MACV virus as well as vesicular stomatitis virus (VSV) were generated and titers determined as described (20,21). Pseudotypes (multiplicity of infection of 0.1) were preincubated with the compounds (concentration was 50 M for individual compounds and 5 M for individual compounds in mixtures of 4 -10) for 45 min and then added to cell monolayers in 96-well plates in presence of drug.…”

Section: Methodsmentioning

confidence: 99%

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Unique Small Molecule Entry Inhibitors of Hemorrhagic Fever Arenaviruses

Lee

Rojek

Spiropoulou

et al. 2008

Journal of Biological Chemistry

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100

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“…The cells were then chilled on ice and detached with enzyme-free cell dissociation, and the cell surface expression of ␣-DG and TfR1 was assessed by flow cytometry (9,20).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Unique Small Molecule Entry Inhibitors of Hemorrhagic Fever Arenaviruses

Lee

Rojek

Spiropoulou

et al. 2008

Journal of Biological Chemistry

Self Cite

100

View full text Add to dashboard Cite

show abstract

“…In contrast, the established human B-and T-cell lines tested express a form of α-DG that lacks a detectable functional modifi cation, rendering these cell types highly resistant (Rojek et al, 2007). DCs express the greatest amount of α-DG on their surfaces (Sevilla et al, 2000).…”

Section: Receptor Controlmentioning

confidence: 99%

“…Arenavirus binding to the α-DG receptor critically depends on its protein O-mannosylation, but not on more generic O-linked glycans (Rojek et al, 2007).…”

Section: Receptor Controlmentioning

confidence: 99%

Lymphocytic choriomeningitis virus: ways to establish and maintain non-cytolytic persistent infection

Labudová¹,

Pastorek²,

Pastoreková³

2016

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Summary. -Lymphocytic choriomeningitis virus (LCMV) is a prototype virus of the Arenaviridae family that is attracting considerable attention both as an important experimental model system to study acute and persistent viral infections, and as a neglected human pathogen of clinical signifi cance. Notably, LCMV is capable of persisting in an infected host, and escaping the immune system. Here we describe the strategies used by the virus to establish and maintain long-term infection in vitro and/or persistent infection in vivo. We discuss how the viral components (RNA, nucleoprotein, glycoprotein, Z protein) manipulate the host cell machinery to facilitate survival and spread of the virus without disturbing the basal cellular processes. Deep understanding of these strategies is inevitable for the development of approaches towards restricting the virus spread and/or preventing its harmful reactivation. Th is review summarizes the current status in this area and presents ideas emerging from existing data.Keywords: lymphocytic choriomeningitis virus; arenaviruses; persistent infection E-mail: virulama@savba.sk; phone: +421-2-59302439. Abbreviations: ATF6 = activating transcription factor 6; DC = dendritic cells; DG = dystroglycan; ECM = extracellular matrix; eIF-4E/G = eukaryotic translation initiation factor 4E or 4G; eIF-4G = eukaryotic translation initiation factor 4G; GAPDH = glyceraldehyd-3-phosphate dehydrogenase; GP1,2 = glycoprotein 1, 2; GPC = glycoprotein precursor; IRE1 = inositol-requiring protein 1; IRF-3 = IFN regulatory factor 3; PERK = PKR-like ER kinase; PML = promyelocytic leukemia; UPR = unfolded protein response; ZP = Z protein

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“…в эту модификацию включены многие кле-точные энзимы, в том числе клеточная ацетилглю-козамин трансфераза, протеин-о-маннозил транс-фераза, протеин-о-манноза трансфераза, фукутин и родственные им белки [21]. в исследованиях, прове-денных с вирусом лхм, выявлено, что при инфици-ровании in vivo гликозилирование не является кри-тическим моментом для развития инфекции.…”

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