Olea europaea L. cv. Chetoui leaf and stem hydromethanolic extracts suppress proliferation and promote apoptosis via caspase signaling on human multiple myeloma cells

Limam, Inès; Abdelkarim, Mohamed; Chahbi, Ahlem; Fathallah, Mayssa; Elkahoui, Salem; Aissa-Fennira, Fatma Ben

doi:10.1016/j.eujim.2020.101145

Cited by 12 publications

(14 citation statements)

References 50 publications

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“…Cytotoxicity of E. arenarium Guss extracts against U266 cells was assessed using MTT and Trypan blue assays as previously described 5 . Trypan blue exclusion assay was first performed to evaluate viability in a dose/time‐dependent manner.…”

Section: Methodsmentioning

confidence: 99%

“…Cell cycle (sub‐G1, G1, S, and G2/M) analysis was performed using FXCycle PI/RNase Staining Solution as previously described 5 . Summarily, U266 cells were treated or not treated (control, methanol 0.5%) with 100 μg/ml of E. arenarium Guss extracts for 24 h. Then fixed cells were incubated with the Staining Solution and were analysed with a flow cytometer.…”

Section: Methodsmentioning

confidence: 99%

“…All of these limitations support the need to explore new therapeutic strategies to overcome drug resistance 3 4 against various diseases, including cancer 5‐7 . In recent decades, there has been a growing interest in biomolecules in cancer research 8,9 .…”

Section: Introductionmentioning

confidence: 99%

“…In recent decades, there has been a growing interest in biomolecules in cancer research 8,9 . Plant extracts may be used to increase therapeutic efficacy by sensitizing malignant cells to drugs such as hematologic malignancies 1,3,5,10 …”

Section: Introductionmentioning

confidence: 99%

“…Tunisian people use in their pharmacological systems influenced by Arab and African culture, a wide variety of traditional medicinal herbs. To this end, numerous studies have been carried out to enhance the potential benefits of local medicinal plants 5,12,13 …”

Section: Introductionmentioning

confidence: 99%

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Hydromethanolic root and aerial part extracts from Echium arenarium Guss suppress proliferation and induce apoptosis of multiple myeloma cells through mitochondrial pathway

Limam

Aissa-Fennira

Chahbi

et al. 2021

Environmental Toxicology

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Echium arenarium Guss is a Mediterranean plant traditionally used in healing skin wound and it was reported exhibiting potent antioxidant, antibacterial, and antiparasitic activities. However, antitumoral activities of this plant have not yet been explored. Here we investigated for the first time, root (EARE) and aerial part (EAAPE) extracts of E. arenarium Guss to examine cytotoxicity and apoptosis activation pathway on U266 human multiple myeloma (MM) cell line. We demonstrated that EARE and EAAPE decreased U266 cell viability in a dose dependent manner. Based on 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, EARE was significantly two times more efficient (IC50 value 41 μg/ml) than EAAPE (IC50 value 82 μg/ml) considering 48 h of treatment. Furthermore, after 24 h of exposure to 100 μg/ml of EARE or EAAPE, cell cycle showed remarkable increase in sub‐G1 population and a decrease of U266 cells proportion in G1 phase. In addition, EARE increased cell percentage in S phase. Moreover, analysis revealed that EAAPE or EARE induced apoptosis of U266 cells after 24 h of treatment. Interestingly, depolarization of mitochondrial membrane potential and activation of caspase 3/7 were demonstrated in treated U266 cells. Phytochemical analysis of E. arenarium extracts showed that EARE exhibited the highest content of total phenolic content. Interestingly, six phenolic compounds were identified. Myricitrin was the major compound in EARE, followed by luteolin 7‐O‐glucoside, resorcinol, polydatin, Trans‐hydroxycinnamic acid, and hyperoside. These findings proved that an intrinsic mitochondria‐mediated apoptosis pathway probably mediated the apoptotic effects of E. arenarium Guss extracts on U266 cells, and this will suggest several action plans to treat MM.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hydromethanolic root and aerial part extracts from Echium arenarium Guss suppress proliferation and induce apoptosis of multiple myeloma cells through mitochondrial pathway

Limam

Aissa-Fennira

Chahbi

et al. 2021

Environmental Toxicology

Self Cite

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Caspase‐driven cancer therapies: Navigating the bridge between lab discoveries and clinical applications

Allani,

Akhilesh,

Tiwari

2024

Cell Biochemistry & Function

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Apoptosis is the cell's natural intrinsic regulatory mechanism of normal cells for programmed cell death, which plays an important role in cancer as a classical mechanism of tumor cell death causing minimal inflammation without causing damage to other cells in the vicinity. Induction of apoptosis by activation of caspases is one of the primary targets for cancer treatment. Over the years, a diverse range of natural, synthetic, and semisynthetic compounds and their derivatives have been investigated for their caspase‐mediated apoptosis‐induced anticancer activities. The review aims to compile the preclinical evidence and highlight the critical mechanistic pathways related to caspase‐induced cell apoptosis in cancer treatment. The focus is placed on the key components of the mechanisms, including their chemical nature, and specific attention is given to phytochemicals derived from natural sources and synthetic and semisynthetic compounds. 180+ compounds from the past two decades with potential as anticancer agents are discussed in this review article. By summarizing the current knowledge and advancements in this field, this review provides a comprehensive overview of potential therapeutic strategies targeting apoptosis in cancer cells. The findings presented herein contribute to the ongoing efforts to combat cancer and stimulate further research into the development of effective and targeted anticancer therapies.

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Anticancer effects of Artemisia campestris extract on acute myeloid leukemia cells: an ex vivo study

Kharrat,

Lakhal,

Souia

et al. 2024

Med Oncol

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Olea europaea L. cv. Chetoui leaf and stem hydromethanolic extracts suppress proliferation and promote apoptosis via caspase signaling on human multiple myeloma cells

Cited by 12 publications

References 50 publications

Hydromethanolic root and aerial part extracts from Echium arenarium Guss suppress proliferation and induce apoptosis of multiple myeloma cells through mitochondrial pathway

Hydromethanolic root and aerial part extracts from Echium arenarium Guss suppress proliferation and induce apoptosis of multiple myeloma cells through mitochondrial pathway

Caspase‐driven cancer therapies: Navigating the bridge between lab discoveries and clinical applications

Anticancer effects of Artemisia campestris extract on acute myeloid leukemia cells: an ex vivo study

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