Oleanolic Acid: Extraction, Characterization and Biological Activity

Castellano, José María; Ramos-Romero, Sara; Perona, Javier S.

doi:10.3390/nu14030623

Cited by 153 publications

(90 citation statements)

References 199 publications

(310 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the last two decades, OA has been subject to intensive research. A number of dietary sources of these biomolecules and multiple beneficial effects in the prevention of chronic disorders in which oxidative stress and inflammation have been described [ 66 ]. The anti-inflammatory activity of this triterpene in different pathophysiological scenarios is well documented [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Lipophilic Bioactive Compounds Transported in Triglyceride-Rich Lipoproteins Modulate Microglial Inflammatory Response

Espinosa

Castellano

Garcia-Rodriguez

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Microglial cells can contribute to Alzheimer’s disease by triggering an inflammatory response that leads to neuronal death. In addition, the presence of amyloid-β in the brain is consistent with alterations in the blood–brain barrier integrity and triglyceride-rich lipoproteins (TRL) permeation. In the present work, we used lab-made TRL as carriers of lipophilic bioactive compounds that are commonly present in dietary oils, namely oleanolic acid (OA), α-tocopherol (AT) and β-sitosterol (BS), to assess their ability to modulate the inflammatory response of microglial BV-2 cells. We show that treatment with lab-made TRL increases the release and gene-expression of IL-1β, IL-6, and TNF-α, as well as NO and iNOS in microglia. On the other hand, TRL revealed bioactive compounds α-tocopherol and β-sitosterol as suitable carriers for oleanolic acid. The inclusion of these biomolecules in TRL reduced the release of proinflammatory cytokines. The inclusion of these biomolecules in TRL reduced the release of proinflammatory cytokines. AT reduced IL-6 release by 72%, OA reduced TNF-α release by approximately 50%, and all three biomolecules together (M) reduced IL-1β release by 35% and TNF-α release by more than 70%. In addition, NO generation was reduced, with the inclusion of OA by 45%, BS by 80% and the presence of M by 88%. Finally, a recovery of the basal glutathione content was observed with the inclusion of OA and M in the TRL. Our results open the way to exploiting the neuro-pharmacological potential of these lipophilic bioactive compounds through their delivery to the brain as part of TRL.

show abstract

Section: Discussionmentioning

confidence: 99%

Lipophilic Bioactive Compounds Transported in Triglyceride-Rich Lipoproteins Modulate Microglial Inflammatory Response

Espinosa

Castellano

Garcia-Rodriguez

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Castellano et al demonstrated that oleanolic acid could inhibit the release of inflammatory factors (IL-1β, TNF-α, IL-6, etc.) under various inflammation and oxidative stress scenarios ( Castellano et al, 2022 ). The molecular mechanisms mentioned above are all involved in AP, which might help decipher why DCP works better at regulating oxidative stress and inflammation in the duodenum.…”

Section: Discussionmentioning

confidence: 99%

Dao-Chi Powder Ameliorates Pancreatitis-Induced Intestinal and Cardiac Injuries via Regulating the Nrf2-HO-1-HMGB1 Signaling Pathway in Rats

et al. 2022

View full text Add to dashboard Cite

Dao-Chi powder (DCP) has been widely used in the treatment of inflammatory diseases in the clinical practice of traditional Chinese medicine, but has not been used in acute pancreatitis (AP). This study aimed to evaluate the effect of DCP on severe AP (SAP) and SAP-associated intestinal and cardiac injuries. To this end, an SAP animal model was established by retrograde injection of 3.5% taurocholic acid sodium salt into the biliopancreatic ducts of rats. Intragastric DCP (9.6 g/kg.BW) was administered 12 h after modeling. The pancreas, duodenum, colon, heart and blood samples were collected 36 h after the operation for histological and biochemical detection. The tissue distributions of the DCP components were determined and compared between the sham and the SAP groups. Moreover, molecular docking analysis was employed to investigate the interactions between the potential active components of DCP and its targets (Nrf2, HO-1, and HMGB1). Consequently, DCP treatment decreased the serum levels of amylase and the markers of gastrointestinal and cardiac injury, further alleviating the pathological damage in the pancreas, duodenum, colon, and heart of rats with SAP. Mechanistically, DCP rebalanced the pro-/anti-inflammatory cytokines and inhibited MPO activity and MDA levels in these tissues. Furthermore, Western blot and RT-PCR results showed that DCP intervention enhanced the expression of Nrf2 and HO-1 in the duodenum and colon of rats with SAP, while inhibiting the expression of HMGB1 in the duodenum and heart. HPLC-MS/MS analysis revealed that SAP promoted the distribution of ajugol and oleanolic acid to the duodenum, whereas it inhibited the distribution of liquiritigenin to the heart and ajugol to the colon. Molecular docking analysis confirmed that the six screened components of DCP had relatively good binding affinity with Nrf2, HO-1, and HMGB1. Among these, oleanolic acid had the highest affinity for HO-1. Altogether, DCP could alleviated SAP-induced intestinal and cardiac injuries via inhibiting the inflammatory responses and oxidative stress partially through regulating the Nrf2/HO-1/HMGB1 signaling pathway, thereby providing additional supportive evidence for the clinical treatment of SAP.

show abstract

“…An elaborate review was published in 2016 regarding the molecular mechanisms of OA antineoplastic activity in different types of cancer ( Figure 9 ) [ 121 ]. Another recently published review by Castellano et al [ 122 ] depicts the molecular mechanisms involved in the antihypertensive, antihyperglycemic, and anti-inflammatory activity of OA.…”

Section: Oleanolic Acidmentioning

confidence: 99%

Recent Advances Regarding the Molecular Mechanisms of Triterpenic Acids: A Review (Part I)

Mioc

Milan

Malița

et al. 2022

IJMS

View full text Add to dashboard Cite

Triterpenic acids are phytocompounds with a widespread range of biological activities that have been the subject of numerous in vitro and in vivo studies. However, their underlying mechanisms of action in various pathologies are not completely elucidated. The current review aims to summarize the most recent literature, published in the last five years, regarding the mechanism of action of three triterpenic acids (asiatic acid, oleanolic acid, and ursolic acid), corelated with different biological activities such as anticancer, anti-inflammatory, antidiabetic, cardioprotective, neuroprotective, hepatoprotective, and antimicrobial. All three discussed compounds share several mechanisms of action, such as the targeted modulation of the PI3K/AKT, Nrf2, NF-kB, EMT, and JAK/STAT3 signaling pathways, while other mechanisms that proved to only be specific for a part of the triterpenic acids discussed, such as the modulation of Notch, Hippo, and MALAT1/miR-206/PTGS1 signaling pathway, were highlighted as well. This paper stands as the first part in our literature study on the topic, which will be followed by a second part focusing on other triterpenic acids of therapeutic value.

show abstract

Oleanolic Acid: Extraction, Characterization and Biological Activity

Cited by 153 publications

References 199 publications

Lipophilic Bioactive Compounds Transported in Triglyceride-Rich Lipoproteins Modulate Microglial Inflammatory Response

Lipophilic Bioactive Compounds Transported in Triglyceride-Rich Lipoproteins Modulate Microglial Inflammatory Response

Dao-Chi Powder Ameliorates Pancreatitis-Induced Intestinal and Cardiac Injuries via Regulating the Nrf2-HO-1-HMGB1 Signaling Pathway in Rats

Recent Advances Regarding the Molecular Mechanisms of Triterpenic Acids: A Review (Part I)

Contact Info

Product

Resources

About