Oleanolic acid rejuvenates testicular function through attenuating germ cell DNA damage and apoptosis via deactivation of NF-κB, p53 and p38 signalling pathways

Zhao, Haixia; Liu, Jing; Li, Song; Zhen-cai, Liu; Han, Gencheng; Yuan, Ding; Wang, Ting; Dun, Yaoyan; Zhou, Zhi; Liu, Zhaoqi; Wang, Yongjun; Zhang, Changcheng

doi:10.1111/jphp.12668

Cited by 22 publications

(14 citation statements)

References 40 publications

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“…Activation of the NF‐κB/Bax pathway can cause apoptosis. The inactive form of NF‐κB resides in the cytoplasm and associates or links with its natural biological inhibitor, IκB.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D₃supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B

Liu

Wang

et al. 2018

J of Diabetes Invest

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Aims/Introduction: Vitamin D 3 deficiency can lead to male hypogonadism in diabetes mellitus, but the target organs and the mechanism driving the disorder are unclear. This experiment was designed to study the relationship between vitamin D 3 deficiency and hypogonadism in diabetes mellitus. Materials and Methods: Rats with streptozotocin-induced diabetes were randomly divided into four groups and treated with different doses of vitamin D 3 : blank (no vitamin D 3 ), low (0.025 lg/kg/day), high (0.1 lg/kg/day), high (0.1 lg/kg/day) and with bisphenol A diglycidyl ether (peroxisome proliferator-activated receptor gamma inhibitor 30 mg/kg/ day). They were compared with wild-type rats. Results: After 12 weeks, the vitamin D 3 supplements had partially restored testicular pathological changes, as shown by reduced testicular fibrosis related to downregulation transforming growth factor beta 1 and apoptosis related to downregulation of nuclear factor kappa B, but not the pituitary gland. The expression of peroxisome proliferator-activated receptor gamma, which can inhibit transforming growth factor beta 1 and nuclear factor kappa B, was significantly increased after treatment with vitamin D 3 . Conclusions: These results suggest that treatment with vitamin D 3 can improve testicular function in diabetic rats through the peroxisome proliferator-activated receptor gamma/transforming growth factor beta 1/nuclear factor kappa B signaling pathway.

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“…Activation of the NF‐κB/Bax pathway can cause apoptosis. The inactive form of NF‐κB resides in the cytoplasm and associates or links with its natural biological inhibitor, IκB.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D₃supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B

Liu

Wang

et al. 2018

J of Diabetes Invest

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“…However, extensive DNA damage results in activation of the cell death mechanisms such as apoptosis and necrosis [ 48 ]. Previous studies show that ROS and NF-κB signaling mechanisms are involved in DNA damage [ 27 , 49 , 50 ]. Our study shows that fluoride induces nuclear DNA fragmentation in a time- and dose- dependent manner.…”

Section: Discussionmentioning

confidence: 99%

“…Nuclear factor κB (NF-κB) signaling plays an important role in DNA damage response and apoptosis in diverse cell type [ 23 – 27 ]. Moreover, fluoride promotes expression of NF-κB pathway genes and activation of the NF-κB signaling pathway [ 28 – 30 ].…”

Section: Introductionmentioning

confidence: 99%

Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-κB signaling

et al. 2017

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In this study, we investigated the roles of reactive oxygen species (ROS) and nuclear factor-κB (NF-κB) signaling in sodium fluoride-induced DNA damage and apoptosis in mouse splenocytes. Intragastric administration of 12, 24 or 48 mg/kg sodium fluoride resulted in a time- and dose-dependent increase in DNA fragmentation and apoptosis in mouse splenocytes on days 21 and 42. High ROS levels correlated with increased levels of phosphorylated IκB kinase and NF-κB p65 and decreased levels of inhibitory kappa B protein in splenocytes from mice treated with sodium fluoride. Moreover, splenocytes from sodium fluoride-treated mice showed high expression of pro-apoptotic proteins, including Bim, Bax, Bak, caspase-3 and poly ADP-ribose polymerase, and low expression of the anti-apoptotic proteins BcL-2 and BcL-xL. These results show that sodium fluoride induces apoptosis in mouse splenocytes by enhancing ROS-dependent NF-κB signaling.

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“…Djeziri et al [20] reported that oleanolic acid exerts protective effects on obesity, including a decrease in body and adipose tissue weight and inflammatory cytokine production. In the reproductive experimental field, Zhao et al [32] showed that oleanolic acid effectively rejuvenated testicular function by reducing DNA damage, apoptosis, and inflammatory cytokine production, indicating a potential for the prevention of aging-related testicular dysfunction. On the other hand, as OleaVita contains many other components, such as oleuropein and polyphenolic compounds, further studies are necessary warranted to understand the exacts effect of OleaVita.…”

Section: Discussionmentioning

confidence: 99%

Olive Leaf Extract (OleaVita) Suppresses Inflammatory Cytokine Production and NLRP3 Inflammasomes in Human Placenta

et al. 2019

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The placenta is essential for pregnancy and produces both pro-inflammatory and anti-inflammatory cytokines. Excessive production of inflammatory cytokines, involving interleukin-1β (IL-1β), IL-6, and IL-8, from placental tissues is associated with pregnancy complications. Olive leaf extract has several health benefits, including anti-inflammatory functions. OleaVita is a new commercial olive leaf extract; it is hypothesized to suppress placental inflammation. In human placental tissue culture, OleaVita treatment inhibited the secretion of inflammatory cytokines and NF-κB p65 protein expression. OleaVita also suppressed toll-like receptor ligands-induced IL-1β secretion in human placental tissues. IL-1β is regulated by the NLRP3 inflammasomes, a pivotal regulator of various diseases. OleaVita significantly decreased NLRP3 and pro-IL-1β protein expression, suggesting that it has an inhibitory effect on NLRP3 inflammasome activation. Thus, OleaVita is beneficial as an inhibitor of inflammation and NLRP3 inflammasome activation, and may be used as a supplement for the treatment and prevention of inflammatory diseases.

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Oleanolic acid rejuvenates testicular function through attenuating germ cell DNA damage and apoptosis via deactivation of NF-κB, p53 and p38 signalling pathways

Abstract: Oleanolic acid effectively rejuvenates testicular function via attenuating germ cell DNA damage and apoptosis through deactivation of NF-κB, p53 and p38 signalling pathways.

Cited by 22 publications

References 40 publications

Vitamin D₃supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B

Vitamin D₃supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B

Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-κB signaling

Olive Leaf Extract (OleaVita) Suppresses Inflammatory Cytokine Production and NLRP3 Inflammasomes in Human Placenta

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Oleanolic acid rejuvenates testicular function through attenuating germ cell DNA damage and apoptosis via deactivation of NF-κB, p53 and p38 signalling pathways

Abstract: Oleanolic acid effectively rejuvenates testicular function via attenuating germ cell DNA damage and apoptosis through deactivation of NF-κB, p53 and p38 signalling pathways.

Cited by 22 publications

References 40 publications

Vitamin D3supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B

Vitamin D3supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B

Sodium fluoride induces apoptosis in mouse splenocytes by activating ROS-dependent NF-κB signaling

Olive Leaf Extract (OleaVita) Suppresses Inflammatory Cytokine Production and NLRP3 Inflammasomes in Human Placenta

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Vitamin D₃supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B

Vitamin D₃supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B