Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABA<sub>A</sub> Receptors

Abstract

A mixture of eight fatty acids (linoleic, oleic, palmitic, stearic, myristic, elaidic, lauric, and palmitoleic acids) at similar concentrations that have been identified in human amniotic fluid exerts anxiolytic-like effects similar to diazepam in adult Wistar rats through actions at γ-aminobutyric acid-A (GABA A) receptors, but unknown is whether any of these fatty acids exerts a predominant action over the others in infant rats. Of these fatty acids, some actions of oleic acid have already been identified, and it is one of the most abundant in amniotic fluid. Therefore, the aim of this study was to explore the effect of oleic acid on anxiety-like behavior and motoric activity in infant rats. To explore sedative actions, 28-day-old Wistar rats received 80-320 µg oleic acid or a sedative dose of diazepam (5 mg/kg). In a dose-response study, other groups of rats were injected with 10-80 µg oleic acid or 1 mg/kg diazepam. In an interaction study, rats that received oleic acid were pretreated with the GABA A receptor antagonists picrotoxin or flumazenil to explore the participation of this receptor in the effects of oleic acid on behavior in the elevated plus maze, rotarod test, and open field test. Oleic acid produced sedative effects but did not exert any anxiolytic-like actions. Hypoactivity and motor incoordination that were induced by oleic acid were blocked by flumazenil and picrotoxin. In conclusion, oleic acid reduced locomotor activity and motor incoordination through actions at the GABA A receptor.

Sensitivity to diazepam after a single session of forced swim stress in weaning Wistar rats

“…In the present study, the DZP-5 group had the smallest number of total crossings (H 4 = 11.214, p = 0.024) and the smallest number of crossings in the central zone (H 4 = 10.067, p = 0.039) compared to other groups (control, DZP-0.1, DZP-0.5, and DZP-1; Table I). This hypoactivity illustrates a seda- tive effect of 5 mg kg -1 diazepam, an effect consistent with a previous study that found that the 5 mg kg -1 dose increased sedation scores in PND28 rats (11). This finding in the DZP-5 group suggests that 5 mg kg -1 should not be administered in weaning rats because it is sedative rather than anxiolytic.…”

A Fatty Acids Mixture Reduces Anxiety-Like Behaviors in Infant Rats Mediated by GABAA Receptors

“…Diazepam (Valium 10, Hoffman-Roche, Basel, Switzerland) was used as the reference anxiolytic drug and injected intraperitoneally at a dose of 1 mg/kg dissolved in isotonic saline solution (v2) in a volume of 1 ml/300 g. This dose was selected because it produces anxiolytic-like effects in 28-day-old rats [ 19 , 23 , 24 ].…”

Topamax® and Ginger Oil Potential Neurotoxicity And Their Interaction in Mice Thru Oxidative Stress and Inflammatory Markers, Neurotransmitters Expression and Immunohistochemical Measurements

“…Also, many studies reported that the alteration of α5 GABAAR levels have been observed in neurodevelopmental disorders [77,78]. Additionally, down-regulation of Gabra1, 3 and 5 mRNA levels was reported in Alzheimer's disease (AD) patients [79]. In the present study, the expression level of three α subunit-encoding genes, Gabra1, Gabra3 and Gabra5, were evaluated in response to Topamax and ginger oil and their interaction.…”