Óleo De Coco, Uma Alternativa De Diafanizador Na Técnica Histológica

Abstract

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Replacement of xylene with extra virgin coconut oil in the clearing step of the histological routine

“…According to Nunez and Cinsa (2016) the staining process is a step important, and fundamental since it dyes the tissue components and consequently allows its visualization.To carry out the staining of the tissues, it is essential to remove the paraffin, which is usually preceded by xylene and alcohol baths (Tolosa et al, 2003;Timm , 2005 andNunez &Cinsa, 2016). In our study, paraffin removal was also (1992) removed the paraffin on the slides, with coconut oil at 60 o C. Regardless of the methodology used, the miscibility of oils with xylene and paraffin is high and does not interfere with staining with H/E (Rasmussen et al, 1992;Premala tha et al, 2013;Sermadi et al, 2014;Swamy et al, 2015;Indu et al, 2016;Ashitha, 2018;Chandraker et al, 2018;Abreu et al, 2019;Sermadi et al 2019;Career et al 2019Tsamiya et al 2021Akpulu et al, 2021). Regarding other histological staining techniques, Rasmussen et al (1992) showed no differences in histochemical and immunohistochemical staining, a fact corroborated by Sermadi et al (2014) who found that staining with PAS (periodic acid Schiff) is not compromised by the use of coconut oil or olive oil (Sermadi et al, 2019).…”

“…Udonkang et al (2014) evaluated palm oil as a clarifying agent and found small differences compared to xylene, without compromising tissue quality; Sermadi et al (2014) compared coconut oil with xylene as a clarifying agent and did not show impairment of histological quality; Indu et al (2014) proposed cedar oil as an effective, ecological and safe alternative to xylene as a deparaffinization agent in the histopathological laboratory; Swamy et al (2015) successfully used carrot oil, pine oil, rose oil and olive oil (olive oil) in histological processing, and found that the four oils have a capacity to clear tissues similar to xylene, being economical oils and that do not compromise histological staining; pine oil was superior in its physical and whitening properties; Digala et al (2017) report that the results obtained with coconut oil and peanut oil are better than xylene in the processing of tissues without health risks; Ashitha (2018) used coconut oil and palm oil in the clearing step and found that both oils, with an advantage over coconut oil, have potential as a substitute for xylene; Chandraker et al (2018) reported difficulties replacing xylene with coconut oil, but stated that the histological quality is similar and without risk to health; Ravindran et al (2018) evaluated palm oil and found similarity with xylene, with good histological results, being a product free of toxicity, without risks, non-flammable, biodegradable, economical, easy to handle and readily available, in addition to staining characteristics with hematoxylin staining and eosin (H/E) showed longevity without fading. histological diaphanization processing, given the results obtained in their study; Abreu et al (2019) demonstrated that in the clearing step, the 1:1 solution (xylene and coconut oil) was satisfactory and meets the specificities of the histological routine; Akpulu et al (2021) recommended eucalyptus oil as an effective agent in the dewaxing process without compromising the staining step and Tsamiya et al (2021) stated that olive oil, clove oil, and peanut oil can clear tissues when compared to those clarified with xylene, also considering their reduced cost, availability, beneficial effects on health and safety for the environment.…”

Substituição do xilol por óleo de coco extravirgem na etapa de diafanização da rotina histológica

“…Independentemente da metodologia usada a miscibilidade dos óleos com o xilol e a parafina é grande e não interfere na coloração com H/E (Rasmussen et al, 1992;Premalatha et al, 2013;Sermadi et al, 2014;Swamy et al, 2015;Indu et al, 2016;Ashitha, 2018;Chandraker et al, 2018;Abreu et al, 2019;Sermadi et al 2019;Carreira et al 2019Tsamiya et al 2021Akpulu et al, 2021). Em relação a outras técnicas de tingimento histológico Rasmussen et al (1992) não evidenciaram diferenças nas colorações histoquímica e imuno-histoquímica, fato corroborado por Sermadi et al (2014) que constataram que a coloração com PAS (ácido periódico de Schiff) não é comprometida pelo uso do óleo de coco e nem com azeite (Sermadi et al, 2019).…”