Oleosins and Oil Bodies in Seeds and Other Organs

Huang, Anthony H. C.

doi:10.1104/pp.110.4.1055

Cited by 505 publications

(389 citation statements)

References 16 publications

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“…The acyl chains of the phospholipid monolayer are embedded in the TAG interior of the oil body. Oleosin is a structural protein that is thought to prevent coalescence of oil bodies during seed dehydration (Huang, 1996). That oil bodies originate from the endoplasmic reticulum is consistent with the finding that enzymes of TAG synthesis, including DGAT, are present in microsomal membrane fractions, which are known to contain vesicles of endoplasmic reticulum (Kwanyuen and Wilson, 1986).…”

supporting

confidence: 63%

“…Not only do they both store TAG, but plastoglobuli also appear to be coated with a structural protein termed fibrillin or plastid lipidassociated-protein, which is analogous to oil body oleosin (Pozueta-Romero et al, 1997;Kessler et al, 1999;Rey et al, 2000). Fibrillin and oleosin are thought to prevent coalescence of plastoglobuli and oil bodies, respectively (Huang, 1996;Rey et al, 2000). Moreover, recent experiments with transgenic plants in which fibrillin was overexpressed have indicated that the availability of fibrillin regulates the formation of plastoglobuli in much the same way that oleosin regulates the formation of oil bodies (Huang, 1992;Rey et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

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A Role for Diacylglycerol Acyltransferase during Leaf Senescence

2002

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Lipid analysis of rosette leaves from Arabidopsis has revealed an accumulation of triacylglycerol (TAG) with advancing leaf senescence coincident with an increase in the abundance and size of plastoglobuli. The terminal step in the biosynthesis of TAG in Arabidopsis is catalyzed by diacylglycerol acyltransferase 1 (DGAT1; EC 2.3.1.20). When gel blots of RNA isolated from rosette leaves at various stages of development were probed with the Arabidopsis expressed sequence tag clone, E6B2T7, which has been annotated as DGAT1, a steep increase in DGAT1 transcript levels was evident in the senescing leaves coincident with the accumulation of TAG. The increase in DGAT1 transcript correlated temporally with enhanced levels of DGAT1 protein detected immunologically. Two lines of evidence indicated that the TAG of senescing leaves is synthesized in chloroplasts and sequesters fatty acids released from the catabolism of thylakoid galactolipids. First, TAG isolated from senescing leaves proved to be enriched in hexadecatrienoic acid (16:3) and linolenic acid (18:3), which are normally present in thylakoid galactolipids. Second, DGAT1 protein in senescing leaves was found to be associated with chloroplast membranes. These findings collectively indicate that diacylglycerol acyltransferase plays a role in senescence by sequestering fatty acids de-esterified from galactolipids into TAG. This would appear to be an intermediate step in the conversion of thylakoid fatty acids to phloem-mobile sucrose during leaf senescence.Diacylglycerol (DAG) acyltransferase (DGAT; EC 2.3.1.20) mediates the final acylation step in the synthesis of triacylglycerol (TAG). It is present in most plant organs, including leaves, petals, fruits, anthers, and developing seeds (Hobbs et al., 1999). In seeds, TAG is thought to be synthesized within the membranes of the endoplasmic reticulum and subsequently released into the cytosol in the form of oil bodies, which bleb from the cytoplasmic surface of the endoplasmic reticulum (Huang, 1992). The stored TAG is localized in the interior of the oil body, and the surfaces of oil bodies are coated with a monolayer of phospholipid associated with oleosin, the major protein of oil bodies. The acyl chains of the phospholipid monolayer are embedded in the TAG interior of the oil body. Oleosin is a structural protein that is thought to prevent coalescence of oil bodies during seed dehydration (Huang, 1996). That oil bodies originate from the endoplasmic reticulum is consistent with the finding that enzymes of TAG synthesis, including DGAT, are present in microsomal membrane fractions, which are known to contain vesicles of endoplasmic reticulum (Kwanyuen and Wilson, 1986). In addition, TAG can be synthesized in vitro in the presence of microsomes isolated from developing seeds (Lacey et al., 1999).Although TAG formation in seeds is believed to occur in the ER, there have been several reports indicating that purified chloroplast envelope membranes from leaves are also capable of synthesizing this storage lipid (Siebe...

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supporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

A Role for Diacylglycerol Acyltransferase during Leaf Senescence

2002

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“…A number of bands also occur at less than 29 kDa, probably corresponding to oleosins (HUANG, 1996). Bands can also be seen at less than 14.4 kDa.…”

Section: Sds-polyacrylamide Gel Electrophoresis (Sds-page)mentioning

confidence: 87%

Study of the proteins in the defatted flour and protein concentrate of baru nuts (Dipteryx alata Vog)

et al. 2012

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Baru (Dipteryx alata Vog.) is an abundant legume in the Brazilian Savanna. Its nuts can be exploited sustainably using its protein and lipid fractions. This study aimed to analyze the proteins of the nuts present in the defatted flour and protein concentrate in terms of their functional properties, the profile of their fractions, and the in vitro digestibility. The flour was defatted with hexane and extracted at the pH of higher protein solubility to obtain the protein concentrate. The electrophoretic profile of the protein fractions was evaluated in SDS-PAGE gel. The functional properties of the proteins indicate the possibility of their use in various foods, like soybeans providing water absorption capacity, oil absorption capacity, emulsifying properties, and foamability. Globulins, followed by the albumins, are the major fractions of the flour and protein concentrate, respectively. Digestibility was greater for the concentrate than for the defatted flour.

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“…2 and 7), we captured in type I infructescences stages prior to and at the onset of seed germinability until full premature germination (prior to dormancy induction), and in types II and III infructescences we captured all stages prior to, during, and after the induction of primary dormancy, including the full premature germination stage. To assign these stages to events during seed development and maturation on the molecular level, we analyzed the expression patterns of well-known marker proteins including oleosin (seed storage accumulation), EM6 (seed maturation), and dehydrin (desiccation tolerance; Close et al, 1993;Huang, 1996;Bies et al, 1998;Crowe et al, 2000;Ruuska et al, 2002;Boudet et al, 2006;Siloto et al, 2006;Leprince and Buitink, 2010). Oleosin accumulation during L. papillosum seed development was enhanced with the onset of premature germinability and early maturation in type I and II infructescences.…”

Section: Discussion Spatiotemporal Maturation Patterns In L Papillosmentioning

confidence: 99%

Spatiotemporal Seed Development Analysis Provides Insight into Primary Dormancy Induction and Evolution of theLepidium DELAY OF GERMINATION1Genes

et al. 2013

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Seed dormancy is a block to the completion of germination of an intact viable seed under favorable conditions and is an adaptive and agronomically important trait. Thus, elucidating conserved features of dormancy mechanisms is of great interest. The worldwide-distributed genus Lepidium (Brassicaceae) is well suited for cross-species comparisons investigating the origin of common or specific early-life-history traits. We show here that homologs of the seed dormancy-specific gene DELAY OF GERMINATION1 (DOG1) from Arabidopsis (Arabidopsis thaliana) are widespread in the genus Lepidium. The highly dormant Lepidium papillosum is a polyploid species and possesses multiple structurally diversified DOG1 genes (LepaDOG1), some being expressed in seeds. We used the largely elongated and well-structured infructescence of L. papillosum for studying primary dormancy induction during seed development and maturation with high temporal resolution. Using simultaneous germination assays and marker protein expression detection, we show that LepaDOG1 proteins are expressed in seeds during maturation prior to dormancy induction. Accumulation of LepaDOG1 takes place in seeds that gain premature germinability before and during the seed-filling stage and declines during the late maturation and desiccation phase when dormancy is induced. These analyses of the Lepidium DOG1 genes and their protein expression patterns highlight similarities and species-specific differences of primary dormancy induction mechanism(s) in the Brassicaceae.

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Oleosins and Oil Bodies in Seeds and Other Organs

Cited by 505 publications

References 16 publications

A Role for Diacylglycerol Acyltransferase during Leaf Senescence

A Role for Diacylglycerol Acyltransferase during Leaf Senescence

Study of the proteins in the defatted flour and protein concentrate of baru nuts (Dipteryx alata Vog)

Spatiotemporal Seed Development Analysis Provides Insight into Primary Dormancy Induction and Evolution of theLepidium DELAY OF GERMINATION1Genes

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