Olfactory channels associated with the Drosophila maxillary palp mediate short- and long-range attraction

Dweck, Hany K.M.; Ebrahim, Shimaa A. M.; Khallaf, Mohammed A.; Koenig, Christopher J.; Farhan, Abu; Stieber, Regina; Weißflog, Jerrit; Svatoš, Aleš; Große-Wilde, Ewald; Knaden, Markus; Hansson, Bill S.

doi:10.7554/elife.14925

Cited by 47 publications

(42 citation statements)

References 31 publications

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“…Therefore, the male‐specific EPG responses to cue lure and raspberry ketone may indicate the presence of male‐specific behavioral attractants in B. depressa . The different olfactory role between the maxillary palps and the antennae has been demonstrated through electrophysiological and behavioral studies in other dipteran species such as D. melanogaster (De Bruyne et al, ; Dweck et al, ), N. bullata (Wasserman & Itagaki, ), A. gambiae (Lu et al, ), C. quinquefasciatus (Syed & Leal, ), and B. tryoni (Verschut et al, ), which suggests that the distinct olfactory function of the MpBs is common across wider range of dipteran species.…”

Section: Discussionmentioning

confidence: 97%

Morphological and functional heterogeneity in olfactory perception between antennae and maxillary palps in the pumpkin fruit fly, Bactrocera depressa

Jeong

Kim

et al. 2019

Arch Insect Biochem Physiol

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The morphology and ultrastructure of the olfactory sensilla on the antennae and maxillary palps were investigated through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and their responses to five volatile compounds were measured using electroantenogram (EAG) and electropalpogram (EPG) techniques in the pumpkin fruit fly, Bactrocera depressa (Shiraki; Diptera: Tephritidae). Male and female B. depressa displayed distinct morphological types of olfactory sensilla in the antennae and maxillary palps, with predominant populations of trichoid, basiconic, and coeloconic sensilla. Basiconic sensilla, the most abundant type of olfactory sensilla in the antennae, could be further classified into two different types. In contrast, the maxillary palps exhibited predominant populations of a single type of curved basiconic sensilla. High‐resolution SEM observation revealed the presence of multiple nanoscale wall‐pores on the cuticular surface of trichoid and basiconic sensilla, indicating that their primary function is olfactory. In contrast, coeloconic sensilla displayed several longitudinal grooves around the sensillum peg. The TEM observation of individual antennal olfactory sensilla indicates that the basiconic sensilla are thin‐walled, while the trichoid sensilla are thick‐walled. The profile of EAG responses of male B. depressa was different from their EPG response profile, indicating that the olfactory function of maxillary palps is different from that of antennae in this species. The structural and functional variation in the olfactory sensilla between antennae and maxillary palps suggests that each plays an independent role in the perception of olfactory signals in B. depressa.

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Section: Discussionmentioning

confidence: 97%

Morphological and functional heterogeneity in olfactory perception between antennae and maxillary palps in the pumpkin fruit fly, Bactrocera depressa

Jeong

Kim

et al. 2019

Arch Insect Biochem Physiol

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“…To ensure a reproducible odour stimulus, we used commercial noni juice in most experiments. In a long-range, wind tunnel assay 34 , D. sechellia displayed significantly higher attraction to the noni source than its sister species (Fig. 1b).…”

Section: Long-and Short-range Olfactory Attraction Of D Sechellia Tomentioning

confidence: 98%

The making of an olfactory specialist

Auer

Khallaf

Silbering

et al. 2019

Preprint

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AbstractThe evolution of animal behaviour is poorly understood. Despite numerous correlations of behavioural and nervous system divergence, demonstration of the genetic basis of interspecific behavioural differences remains rare. Here we develop a novel neurogenetic model, Drosophila sechellia, a close cousin of D. melanogaster that displays profound behavioural changes linked to its extreme host fruit specialisation. Through calcium imaging, we identify olfactory pathways detecting host volatiles. Mutational analysis indicates roles for individual receptors in long- and short-range attraction. Cross-species allele transfer demonstrates that differential tuning of one receptor is important for species-specific behaviour. We identify the molecular determinants of this functional change, and characterise their behavioural significance and evolutionary origin. Circuit tracing reveals that receptor adaptations are accompanied by increased sensory pooling onto interneurons and novel central projection patterns. This work links molecular and neuronal changes to behavioural divergence and defines a powerful model for investigating nervous system evolution and speciation.

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“…We wanted to continue to explore the neural pathways for the ab2like and ab3-like OSNs in D. suzukii, we therefore next documented the circuitry from the antenna towards the antennal lobe (AL) using the single-sensillum backfill neuronal staining technique 29 . By comparing the labeled glomeruli from D. suzukii to the known AL atlas of D. melanogaster (Figure 1 B-E) 30 , we could provide additional support for the ab2-like and ab3-like sensillum type identification, as these two fly species shared identical wiring for these OSNs, despite the observed deviation in ligand spectra for D. suzukii adults.…”

Section: Conservation Of Neuronal Wiring Despite Ligand Spectrum Shiftsmentioning

confidence: 99%

Evolution of a pest: towards the complete neuroethology of Drosophila suzukii and the subgenus Sophophora

Keesey

Zhang

Depetris-Chauvin

et al. 2019

Preprint

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Comparative analysis of multiple genomes has been used extensively to examine the evolution of chemosensory receptors across the genus Drosophila. However, few studies have delved into functional characteristics, as most have relied exclusively on genomic data alone, especially for non-model species. In order to increase our understanding of olfactory evolution, we have generated a comprehensive assessment of the olfactory functions associated with the antenna and palps for Drosophila suzukii as well as several other members of the subgenus Sophophora, thus creating a functional olfactory landscape across a total of 20 species. Here we identify and describe several common elements of evolution, including consistent changes in ligand spectra as well as relative receptor abundance, which appear heavily correlated with the known phylogeny. We also combine our functional ligand data with protein orthologue alignments to provide a high-throughput evolutionary assessment and predictive model, where we begin to examine the underlying mechanisms of evolutionary changes utilizing both genetics and odorant binding affinities. In addition, we document that only a few receptors frequently vary between species, and we evaluate the justifications for evolution to reoccur repeatedly within only this small subset of available olfactory sensory neurons.

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Olfactory channels associated with the Drosophila maxillary palp mediate short- and long-range attraction

Cited by 47 publications

References 31 publications

Morphological and functional heterogeneity in olfactory perception between antennae and maxillary palps in the pumpkin fruit fly, Bactrocera depressa

Morphological and functional heterogeneity in olfactory perception between antennae and maxillary palps in the pumpkin fruit fly, Bactrocera depressa

The making of an olfactory specialist

Evolution of a pest: towards the complete neuroethology of Drosophila suzukii and the subgenus Sophophora

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