Olfactory ensheathing cell transplantation inhibits P2X4 receptor overexpression in spinal cord injury rats with neuropathic pain

Zheng, Zuncheng; Du, Xiaojing; Zhang, Kaigang; Wang, Xiaoyu; Chen, Yuexia; Kuang, Naifeng; Fan, Tao; Sun, Bo

doi:10.1016/j.neulet.2017.04.060

Cited by 20 publications

(9 citation statements)

References 27 publications

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“…The type of spinal cord injury in published studies varied considerably ( Figure 2C ). The most common injury models in the studies were transection in 35 (35.7%) experiments, contusion in 19 (19.4%), compression in 16 (16.3%), demyelination in 15 (15.3%), hemisection in 7 (7.1%), electrolytic in 3 (3.0%) and photochemical in 2 (2.0%) experiments, and in one study it was unclear which SCI model was applied ( Zheng et al, 2017 ). It should be noted that in addition to the heterogeneity in injuries amongst the studies, there was also considerable heterogeneity with regards to the time of application of cells post injury, injection volumes, concentration of cells, number and localization of injections.…”

Section: Resultsmentioning

confidence: 99%

Considering the Cellular Composition of Olfactory Ensheathing Cell Transplants for Spinal Cord Injury Repair: A Review of the Literature

Miah

Ferretti

Choi

2021

Front. Cell. Neurosci.

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Olfactory ensheathing cells (OECs) are specialized glia cells of the olfactory system that support the continual regeneration of olfactory neurons throughout adulthood. Owing to their pro-regenerative properties, OECs have been transplanted in animal models of spinal cord injuries (SCI) and trialed in clinical studies on SCI patients. Although these studies have provided convincing evidence to support the continued development of OEC transplantation as a treatment option for the repair of SCI, discrepancies in the reported outcome has shown that OEC transplantation requires further improvement. Much of the variability in the reparative potential of OEC transplants is due to the variations in the cell composition of transplants between studies. As a result, the optimal cell preparation is currently a subject of debate. Here we review, the characterization as well as the effect of the cell composition of olfactory cell transplantation on therapeutic outcome in SCI. Firstly, we summarize and review the cell composition of olfactory cell preparations across the different species studied prior to transplantation. Since the purity of cells in olfactory transplants might affect the study outcome we also examine the effect of the proportions of OECs and the different cell types identified in the transplant on neuroregeneration. Finally, we consider the effect of the yield of cells on neuroregeneration by assessing the cell dose of transplants on therapeutic outcome.

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Section: Resultsmentioning

confidence: 99%

Considering the Cellular Composition of Olfactory Ensheathing Cell Transplants for Spinal Cord Injury Repair: A Review of the Literature

Miah

Ferretti

Choi

2021

Front. Cell. Neurosci.

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“…At the same time, they also found that the inhibition of the P2 purinoceptor family was an important part of the process. Further studies have revealed that OECs transplantation inhibit P2X2 receptor (Zhao et al, 2015;Zhang et al, 2020c), P2X4 receptor (Zheng et al, 2017;Zhang et al, 2019bZhang et al, , 2020b, and P2X7 receptor (Zhang et al, 2019a(Zhang et al, , 2020a) overexpression mediated NP. Wu et al (2011) found that delayed OECs transplantation also can effectively inhibit hyperalgesia and allodynia after dorsal root injury.…”

Section: Olfactory Ensheathing Cellsmentioning

confidence: 99%

Cell therapy for neuropathic pain

Yin

Zou

Sun

et al. 2023

Front. Mol. Neurosci.

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Neuropathic pain (NP) is caused by a lesion or a condition that affects the somatosensory system. Pathophysiologically, NP can be ascribed to peripheral and central sensitization, implicating a wide range of molecular pathways. Current pharmacological and non-pharmacological approaches are not very efficacious, with over half of NP patients failing to attain adequate pain relief. So far, pharmacological and surgical treatments have focused primarily on symptomatic relief by modulating pain transduction and transmission, without treating the underlying pathophysiology. Currently, researchers are trying to use cell therapy as a therapeutic alternative for the treatment of NP. In fact, mounting pre-clinical and clinical studies showed that the cell transplantation-based therapy for NP yielded some encouraging results. In this review, we summarized the use of cell grafts for the treatment of NP caused by nerve injury, synthesized the latest advances and adverse effects, discussed the possible mechanisms to inform pain physicians and neurologists who are endeavoring to develop cell transplant-based therapies for NP and put them into clinical practice.

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“…The microglial P2X4 receptor plays an important role in neuropathic pain [47,48]. In neuropathic pain models with spared nerve injury or spinal 50 cord injury, the P2X4 receptor is significantly upregulated in activated spinal microglia [49,50]. ATP released from the injured neurons activates microglial P2X4 receptor, to allow calcium influx and membrane depolarization, which further activates p38 mitogen-activated protein kinases (MAPKs) and promotes the synthesis and release of the brain-derived neurotrophic factor (BDNF) [49,51].…”

Section: The P2x 4 Receptor Regulates the Microglial Response In Neur...mentioning

confidence: 99%

The role of purinergic signaling in microglial responses

Gao

2021

Stress and Brain

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Microglia, the primary immune cells in the central nervous system (CNS), are key to the maintenance of homeostasis in the brain parenchyma. In the intact brain, microglia continuously survey the microenvironment with ramified processes. Upon disease and/or damage, they rapidly convert into an amoeboid morphology, move toward the injury, and release cytokines to repair damage or clear debris. Purinergic signaling plays an important role in regulating microglial dynamics and immune responses. Specific purinergic receptors have been shown to participate in different aspects of microglial responses in the normal and diseased brains. In this review, we focus on the role of purinergic signaling-mediated microglial responses under physiological and pathological conditions.

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Olfactory ensheathing cell transplantation inhibits P2X4 receptor overexpression in spinal cord injury rats with neuropathic pain

Cited by 20 publications

References 27 publications

Considering the Cellular Composition of Olfactory Ensheathing Cell Transplants for Spinal Cord Injury Repair: A Review of the Literature

Considering the Cellular Composition of Olfactory Ensheathing Cell Transplants for Spinal Cord Injury Repair: A Review of the Literature

Cell therapy for neuropathic pain

The role of purinergic signaling in microglial responses

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