Olfactory neuroepithelium in the middle turbinate: is there any impact on olfaction function after lateral marsupialization for concha bullosa surgery?

İsmi, Onur; Meşe, Feyzi; Gur, H. Deniz; Gürses, İclal; Vayısoğlu, Yusuf; Görür, Kemal; Özcan, Cengiz

doi:10.1016/j.bjorl.2021.06.005

Cited by 3 publications

(5 citation statements)

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“…Although olfactory epithelium has been inconsistently reported to be found in the middle turbinate, we did not detect any olfactory cells, likely as our biopsy was from the anterior rather than the superomedial portion. 7,70 Additionally, mRNA of tuft/brush cell marker genes was either not found or only a few molecules were detected in the whole cell population. [71][72][73] Lastly, we compared this single cell data which was collected from a healthy individual (SEP310) with data from a sample from patient with schizophrenia (SEP313).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, as the first structures of the upper airway to receive inspired air, they play a role in immune surveillance. [6][7][8] They have been described to be composed of columnar pseudostratified respiratory epithelium containing basal cells, ciliated cells, glandular epithelial cells, and olfactory epithelial cells. [9][10][11] Many of these studies looked at nasal cavity tissue or turbinate tissue in general; however, the specific cellular framework of the middle turbinate has not yet been described.…”

Section: Introductionmentioning

confidence: 99%

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Cell type catalog of middle turbinate epithelium

Mathews

Tung

Foronjy

et al. 2022

Preprint

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Importance: Elucidation of the cellular makeup of the middle turbinate provides a foundation for future studies of pathogenesis of sinonasal disease. Neural progenitors and pluripotent basal cells found in middle turbinate mucosa potentially can be used to develop cellular models to study brain disorders or in regenerative medicine to substitute neuronal tissues. Objective: Single cell RNA-sequencing (scRNA-seq) of middle turbinate mucosa was performed to create the first single cell transcriptome catalog of this part of the human body. Design: Samples were obtained from the head of the middle turbinate from healthy volunteers. After the specimen was prepared per lab protocol, cells were dissociated, suspended, and counted. Single cell libraries were then prepared according to the 10x Genomics protocol and sequenced using NovaSeq 6000 (Illumina). Sequencing data were processed using Cell Ranger, and clustering and gene expression analysis was performed using Seurat. Cell types were annotated using known markers and data from other single cell studies. Setting: Single center, tertiary care center Participants: Healthy volunteer. Intervention(s) (for clinical trials) or Exposure(s) (for observational studies): None. Main Outcome(s) and Measure(s): Identification of cell types of middle turbinate mucosa through expression profiling of single cells using known markers. Results: 14 unique cell types were identified, including serous, goblet, club, basal, ciliated, endothelial, and neural progenitor cells, as well as multiple types of blood cells. Conclusions and Relevance: This catalog provides a comprehensive depiction of the cellular composition of middle turbinate mucosa. By uncovering the cellular stratification of gene expression profiles in healthy middle turbinate epithelium, the groundwork has been laid for further investigation into the molecular pathogenesis and targeted therapy of sinonasal disease.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cell type catalog of middle turbinate epithelium

Mathews

Tung

Foronjy

et al. 2022

Preprint

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“…Also, Sasaki and Nakahara ( 27 ) demonstrated that NPs might contain nerve fibers and respond to neurogenic stimulation. Moreover, it was proven that even the middle turbinate contained olfactory structures in most of the concha bullosa patients ( 13 , 14 ). Hence, based on our study findings, both OCPs and MMPs may contain olfactory neuroepithelium.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies demonstrated that surgical excision of OCPs does not impair and even restore smell function after ESS ( 9 , 10 , 11 ). The OE has been demonstrated to be more anteriorly distributed than it was supposed to be before, and middle turbinate may also contain OE ( 12 , 13 , 14 ). The relationship between the presence of olfactory neuroepithelium in these polyps and olfactory function results has not yet been properly investigated.…”

Section: Introductionmentioning

confidence: 94%

“…In this study, we aimed to investigate the smell function of CRS w/NP patients with OCPs using the smell diskettes olfaction test pre- and post-operatively. We also aimed to investigate the olfactory structures in both MMPs and OCPs by using immunohistochemical analysis of olfactory marker protein (OMP), which is a marker widely used for detecting OE and olfactory structures in the nasal cavity, and we also correlated the staining results with the smell scores of the patients ( 6 , 13 , 14 , 15 ).…”

Section: Introductionmentioning

confidence: 99%

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Olfactory Neuroepithelium in Olfactory Cleft Polyps: Do They Have Any Effect on Olfaction Results After Endoscopic Sinus Surgery?

Özcan¹,

İsmi²,

Meşe³

et al. 2022

TAO

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Objective: How the presence of olfactory structures in olfactory cleft polyps (OCPs) affect olfaction function outcomes after surgical removal has not yet been investigated. In this study we aimed to assess the presence of olfactory structures in OCPs and correlate these findings with olfactory outcomes after endoscopic sinus surgery (ESS). Methods: Twenty seven patients with OCP underwent preoperative topical and systemic steroid treatment and ESS. Biopsies from the middle meatal polyps (MMPs) and OCPs were immunohistochemically analyzed for olfactory marker protein (OMP). The smell diskettes olfaction test was applied to patients at baseline, after steroid treatment (AST) and after ESS. Results: OCPs exhibited OMP staining more commonly and intensely compared to MMPs (p=0.008), however, there were no correlations between OMP staining scores and any of the olfaction scores (p>0.05). Steroid treatment increased smell function significantly (p<0.001), however, there were no significant differences between AST and after ESS smell scores (p=0.17). There were significant correlations between smell gains AST and final smell gains after ESS (r=0.665, p<0.001). Conclusion: OCPs contain olfactory neuroepithelium more commonly and intensely than MMPs in nasal polyp patients. However, surgical importance of this finding is controversial because removal of these polyps did not decrease smell function postoperatively in our study. Nasal polyp patients who will take steroid treatment pre-operatively must be informed that the success of ESS on olfaction depends on the response of the steroid treatment and ESS AST might not have additional favorable effect on smell function.

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