Olfactory stem cells can be induced to express chondrogenic phenotype in a rat intervertebral disc injury model

Murrell, Wayne; Sanford, Emma; Anderberg, Leif; Cavanagh, Brenton; Mackay‐Sim, Alan

doi:10.1016/j.spinee.2009.02.011

Cited by 60 publications

(48 citation statements)

References 43 publications

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“…For this study we grew the ONS cells in standard culture conditions to obtain enough for mRNA, protein and functional analyses as a baseline for future studies of differentiated cells. ONS cells might be even more informative when differentiated into specific neural lineages (Murrell et al, 2005;Murrell et al, 2008;Murrell et al, 2009). Because of their accessibility and relative ease of generation it should be feasible to grow ONS cells from enough patients to investigate cellular sequelae of clinical subtypes (e.g.…”

Section: Dmmbiologistsorg 790mentioning

confidence: 99%

“…Olfactory mucosa contains stem cells that are multipotent and can be propagated as stem or progenitor cells in neurospheres, a hallmark of neural stem cells (Roisen et al, 2001;Murrell et al, 2005;Murrell et al, 2008;Murrell et al, 2009). They can be proliferated in vitro and differentiated into multiple cell types including neurons and glia (Roisen et al, 2001;Murrell et al, 2005;Murrell et al, 2008;Murrell et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Disease-specific, neurosphere-derived cells as models for brain disorders

Matigian

Abrahamsen

Sutharsan

et al. 2010

Disease Models &Amp; Mechanisms

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SUMMARYThere is a pressing need for patient-derived cell models of brain diseases that are relevant and robust enough to produce the large quantities of cells required for molecular and functional analyses. We describe here a new cell model based on patient-derived cells from the human olfactory mucosa, the organ of smell, which regenerates throughout life from neural stem cells. Olfactory mucosa biopsies were obtained from healthy controls and patients with either schizophrenia, a neurodevelopmental psychiatric disorder, or Parkinson's disease, a neurodegenerative disease. Biopsies were dissociated and grown as neurospheres in defined medium. Neurosphere-derived cell lines were grown in serum-containing medium as adherent monolayers and stored frozen. By comparing 42 patient and control cell lines we demonstrated significant disease-specific alterations in gene expression, protein expression and cell function, including dysregulated neurodevelopmental pathways in schizophrenia and dysregulated mitochondrial function, oxidative stress and xenobiotic metabolism in Parkinson's disease. The study has identified new candidate genes and cell pathways for future investigation. Fibroblasts from schizophrenia patients did not show these differences. Olfactory neurosphere-derived cells have many advantages over embryonic stem cells and induced pluripotent stem cells as models for brain diseases. They do not require genetic reprogramming and they can be obtained from adults with complex genetic diseases. They will be useful for understanding disease aetiology, for diagnostics and for drug discovery.

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Section: Dmmbiologistsorg 790mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Disease-specific, neurosphere-derived cells as models for brain disorders

Matigian

Abrahamsen

Sutharsan

et al. 2010

Disease Models &Amp; Mechanisms

Self Cite

186

237

View full text Add to dashboard Cite

show abstract

“…Adult stem cells have a number of advantages for transplantation, as they can be used for autologous transplantation (to resist host rejection) and are less tumorigenic than embryonic stem cells [19]. Adult stem cells from the olfactory mucosa are readily accessible by biopsy and exhibit a broad differentiation potential both in vitro and in transplantation settings [20][21][22]. Transplantation of adult olfactory mucosaderived stem cells (hereafter referred to as olfactory stem cells) has been shown to have beneficial effects in animal models of human disorders relating to a variety of tissues, including Parkinson's disease (transplantation into brain: [23]) and cardiac infarction (transplantation into heart: [22]).…”

Section: Introductionmentioning

confidence: 99%

Functional Effects of Adult Human Olfactory Stem Cells on Early-Onset Sensorineural Hearing Loss

et al. 2011

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Transplantation of exogenous stem cells has been proposed as a treatment to prevent or reverse sensorineural hearing loss. Here, we investigate the effects of transplantation of adult human olfactory mucosa-derived stem cells on auditory function in A/J mice, a strain exhibiting early-onset progressive sensorineural hearing loss. Recent evidence indicates that these stem cells exhibit multipotency in transplantation settings and may represent a subtype of mesenchymal stem cell. Olfactory stem cells were injected into the cochleae of A/J mice via a lateral wall cochleostomy during the time period in which hearing loss first becomes apparent. Changes in auditory function were assessed 1 month after transplantation and compared against animals that received sham injections. Hearing threshold levels in stem cell-transplanted mice were found to be significantly lower than those of sham-injected mice (p < .05) for both click and pure tone stimuli. Transplanted cells survived within the perilymphatic compartments but did not integrate into cochlear tissues. These results indicate that transplantation of adult human olfactory mucosa-derived stem cells can help preserve auditory function during early-onset progressive sensorineural hearing loss. STEM CELLS 2011;29:670-677 Disclosure of potential conflicts of interest is found at the end of this article.

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“…De plus, l'équipe de Roisen aux États-Unis a démontré que la transplantation de cellules souches olfactives nasales humaines améliorait significativement la locomotion de rats devenus paraplé-giques après une section de la moelle épinière [4]. Parallèlement, la capacité de différenciation de ces mêmes cellules humaines en chondrocytes, après transplantation dans un modèle rat de lésion des disques intervertébraux [5], ou en ostéocytes après insertion chez des souris nude, a été mise en évi-dence [2]. Enfin, une troisième équipe a apporté la preuve que : (1) les cellules ques des souris transplantées ont révélé un rétablissement partiel de l'activité électrique hippocampique (transmission cellulaire et potentialisation à long terme), à la base de la récupération des fonctions mnésiques observée.…”

Section: Cellules Souches Olfactives : Restauration Des Fonctions Mnéunclassified