2016
DOI: 10.1016/j.aspen.2016.07.014
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Olfactory stimulation of Apis cerana indica towards different doses of volatile constituents: SEM and EAG approaches

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Cited by 6 publications
(10 citation statements)
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“…We also used pure odor instead of cocktails of molecules such as those emitted by flowers for simplicity. This is a common decision in the research on bee olfaction ( [53][54][55][57][58][59][60] ). Fourth, our only way to identify the onset of the reactions is using an antennal motion threshold, since there was a delay for the odor to reach the insect after it was added to the continuous airflow.…”
Section: Limits Of the Studymentioning
confidence: 99%
See 1 more Smart Citation
“…We also used pure odor instead of cocktails of molecules such as those emitted by flowers for simplicity. This is a common decision in the research on bee olfaction ( [53][54][55][57][58][59][60] ). Fourth, our only way to identify the onset of the reactions is using an antennal motion threshold, since there was a delay for the odor to reach the insect after it was added to the continuous airflow.…”
Section: Limits Of the Studymentioning
confidence: 99%
“…The odors used were benzaldehyde (≥99%, Sigma-Aldrich, Taufkirchen, Germany), 1-butanol (99%, Alfa Aesar, USA), phenylacetaldehyde (95%, Alfa Aesar), + alpha pinene (analytical standard, Fluka, Germany), nonanal (≥95% Sigma-Aldrich), farnesene (mixture of isomers, Sigma-Aldrich), beta caryophyllene (≥80%, Sigma-Aldrich), R+ limonene (analytical standard, Fluka), trans-2-hexen-al (≥95%, Sigma-Aldrich), trans-2-hexen-ol (≥95%, Sigma-Aldrich) and ocimene (≥90%, Sigma-Aldrich). All those odors can be sensed by bees ( [53][54][55][56][57][58][59][60][61] ) (Table 1). All odors were diluted in water except for phenylacetaldehyde that had to be diluted in paraffin oil (puriss, Sigma-Aldrich) for solubility reasons.…”
Section: Odor Preparationmentioning
confidence: 99%
“…The stimulation airflow controller (Syntech, CS‐55) for the EAG experiment can convey two kinds of airflow, a steady airflow and a pulse airflow. Some of the test reagents were selected based on previous work (Bhowmik et al, 2016). In these experiments, the concentration of each reagent varied from 0.1 to 100 μl/ml.…”
Section: Methodsmentioning
confidence: 99%
“…There are also species‐specific variations in the responsiveness of antennae to the same odor; for example, Apis cerana consistently exhibits weaker antennal responses to the mandibular pheromone of the queen bee compared to the antennal responses of Apis mellifera (Dong et al, 2017). The EAG responses of honeybee antennae to varying concentrations of six common plant volatiles were previously evaluated, which showed that these responses can be utilized as odor probes (Bhowmik, Lakare, Sen, & Bhadra, 2016; Lambin, Déglise, & Gauthier, 2005). Honeybees, like vertebrates, respond to negative stimuli (Bateson, Desire, Gartside, & Wright, 2011) both physically and physiologically, which can be used as indicators of their emotions (Paul, Harding, & Mendl, 2005).…”
Section: Introductionmentioning
confidence: 99%
“…The broad types of sensilla in insects and in honey bees are described by (Esslen & Kaissling 1976). Preliminary studies on the olfactory capabilities of honeybee A. cerana indica have been carried out wherein the ultrastructure of the antenna and electroantennogram has been studied (Bhowmik et al 2016). The proteome of antennae of A. mellifera linguistica and A. cerana drones and workers suggest differences in the olfactory capabilities of the two species (Woltedji et al 2012) suggesting differences in olfactory senses.…”
Section: Introductionmentioning
confidence: 99%