Oligodendroglia-derived extracellular vesicles activate autophagy via LC3B/BAG3 to protect against oxidative stress with an enhanced effect for HSPB8 enriched vesicles

Broek, Bram Van den; Wuyts, Charlotte; Sisto, Angela; Pintelon, Isabel; Timmermans, Jean‐Pierre; Somers, Veerle; Timmerman, Vincent; Hellings, Niels; Irobi, Joy

doi:10.1186/s12964-022-00863-x

Cited by 13 publications

(6 citation statements)

References 61 publications

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“…The role of EVs in the proteostasis control appears to be complex. The cells can use EVs to dispose of oligomerised/aggregated or misfolded proteins, or to repair proteotoxic stress-induced protein damage in recipient cells 48,49 . In this aspect, EVs-mediated secretion and intercellular transmission of HSPs were shown to be responsible for maintenance of proteostasis at the organismal level 50 .…”

Section: Discussionmentioning

confidence: 99%

Heat shock protein A2 is a novel extracellular vesicle-associated protein

Sojka

Abramowicz

Adamiec-Organiściok

et al. 2023

Sci Rep

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Abstract70-kDa Heat Shock Proteins (HSPA/HSP70) are chaperones playing a central role in the proteostasis control mechanisms. Their basal expression can be highly elevated as an adaptive response to environmental and pathophysiological stress conditions. HSPA2, one of poorly characterised chaperones of the HSPA/HSP70 family, has recently emerged as epithelial cells differentiation-related factor. It is also commonly expressed in cancer cells, where its functional significance remains unclear. Previously, we have found that proteotoxic stress provokes a decrease in HSPA2 levels in cancer cells. In the present study we found that proteasome inhibition-related loss of HSPA2 from cancer cells neither is related to a block in the gene transcription nor does it relate to increased autophagy-mediated disposals of the protein. Proteotoxic stress stimulated extracellular release of HSPA2 in extracellular vesicles (EVs). Interestingly, EVs containing HSPA2 are also released by non-stressed cancer and normal cells. In human urinary EVs levels of HSPA2 were correlated with the levels of TSG101, one of the main EVs markers. We conclude that HSPA2 may constitute basic components of EVs. Nevertheless, its specific role in EVs and cell-to-cell communication requires further investigation.

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Section: Discussionmentioning

confidence: 99%

Heat shock protein A2 is a novel extracellular vesicle-associated protein

Sojka

Abramowicz

Adamiec-Organiściok

et al. 2023

Sci Rep

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“…Similarly, EVs also regulate autophagy. For example, oligodendroglia‐derived EVs activate autophagy to protect microglia cells against oxidative stress via LC3B/BAG3 pathway with an HSPB8‐dependent enhancement [35].…”

Section: Evs In Different Types Of Pcdmentioning

confidence: 99%

Proteomic characterization of extracellular vesicles in programmed cell death

Xue,

Wong,

Zhao

et al. 2024

Proteomics

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Programmed cell death (PCD) is a fundamental biological process that plays a critical role in cell development, differentiation, and homeostasis. The secretion and uptake of extracellular vesicles (EVs) is one of the important regulatory mechanisms for PCD. EVs are natural membrane structures secreted by cells that contain a variety of proteins, lipids, nucleic acids, and other bioactive molecules. Due to their important roles in intercellular communication and disease progression, there is great interest in studying EVs and their cargo. Different protein components are sorted and packaged in EVs, allowing EVs to perform their functions. The study of EV proteomics helps us understand the role of PCD in the development of diseases. Meanwhile, proteomics is a powerful tool for studying the composition and function of EVs, which assists in the identification, quantification, and profiling of protein components of EVs, and provides insight into the molecular mechanisms involved in PCD and related diseases. In this review, we summarize the characteristics of EV proteomics in different types of PCD, compare different proteomic profiling strategies for EVs, and discuss the impact of EV proteomics on cell function and regulation during PCD, to understand its role in the pathogenesis of related diseases.

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“…In this context, Van den Broek et al. (2022) demonstrate that OL‐EVs are internalized by activated MG and play a pivotal role in maintaining cellular homeostasis during chronic inflammation. They achieve this by enhancing the formation of autophagic vesicles and reducing oxidative stress‐induced apoptotic cell death.…”

Section: Physiological Functions Cell–cell Communication and Ev‐media...mentioning

confidence: 99%

Microglia‐derived extracellular vesicles in homeostasis and demyelination/remyelination processes

Wies Mancini,

Mattera,

Pasquini

et al. 2023

Journal of Neurochemistry

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Microglia (MG) play a crucial role as the predominant myeloid cells in the central nervous system and are commonly activated in multiple sclerosis. They perform essential functions under normal conditions, such as actively surveying the surrounding parenchyma, facilitating synaptic remodeling, engulfing dead cells and debris, and protecting the brain against infectious pathogens and harmful self‐proteins. Extracellular vesicles (EVs) are diverse structures enclosed by a lipid bilayer that originate from intracellular endocytic trafficking or the plasma membrane. They are released by cells into the extracellular space and can be found in various bodily fluids. EVs have recently emerged as a communication mechanism between cells, enabling the transfer of functional proteins, lipids, different RNA species, and even fragments of DNA from donor cells. MG act as both source and recipient of EVs. Consequently, MG‐derived EVs are involved in regulating synapse development and maintaining homeostasis. These EVs also directly influence astrocytes, significantly increasing the release of inflammatory cytokines like IL‐1β, IL‐6, and TNF‐α, resulting in a robust inflammatory response. Furthermore, EVs derived from inflammatory MG have been found to inhibit remyelination, whereas Evs produced by pro‐regenerative MG effectively promote myelin repair. This review aims to provide an overview of the current understanding of MG‐derived Evs, their impact on neighboring cells, and the cellular microenvironment in normal conditions and pathological states, specifically focusing on demyelination and remyelination processes.

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Oligodendroglia-derived extracellular vesicles activate autophagy via LC3B/BAG3 to protect against oxidative stress with an enhanced effect for HSPB8 enriched vesicles

Cited by 13 publications

References 61 publications

Heat shock protein A2 is a novel extracellular vesicle-associated protein

Heat shock protein A2 is a novel extracellular vesicle-associated protein

Proteomic characterization of extracellular vesicles in programmed cell death

Microglia‐derived extracellular vesicles in homeostasis and demyelination/remyelination processes

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