2015
DOI: 10.1007/s00726-015-1923-3
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Oligomeric state and structural stability of two hyperthermophilic β-glucosidases from Thermotoga petrophila

Abstract: The β-glucosidases are enzymes essential for several industrial applications, especially in the field of plant structural polysaccharides conversion into bioenergy and bioproducts. In a recent study, we have provided a biochemical characterization of two hyperthermostable β-glucosidases from Thermotoga petrophila belonging to the families GH1 (TpBGL1) and GH3 (TpBGL3). Here, as part of a continuing investigation, the oligomeric state, the net charge, and the structural stability, at acidic pH, of the TpBGL1 an… Show more

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Cited by 19 publications
(18 citation statements)
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“…PfBGL1 and TpBGL1 are belonging to the family GH1 and have a common (α/β) 8 barrel structure [26,36]. Belonging to the family GH3, TpBGL3 is arranged in three distinct domains: a N-terminal domain (α/β) 8 triose phosphate isomerase barrel, followed by a five-stranded α/β sandwich, and a C-terminal fibronectin type III domain [26]. The archaeal PfBGL1 forms a very stable homotetramer in solution (S1 Fig) and the assembly has a solvent-accessible surface area of 64,660 Å 2 .…”
Section: Resultsmentioning
confidence: 99%
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“…PfBGL1 and TpBGL1 are belonging to the family GH1 and have a common (α/β) 8 barrel structure [26,36]. Belonging to the family GH3, TpBGL3 is arranged in three distinct domains: a N-terminal domain (α/β) 8 triose phosphate isomerase barrel, followed by a five-stranded α/β sandwich, and a C-terminal fibronectin type III domain [26]. The archaeal PfBGL1 forms a very stable homotetramer in solution (S1 Fig) and the assembly has a solvent-accessible surface area of 64,660 Å 2 .…”
Section: Resultsmentioning
confidence: 99%
“…Therefore, several studies have investigated the effect of polyphenolic compounds on enzymatic activity, and as a consequence, contributed to the improvement of cost-effective production of biofuels [1419]. The archaeon Pyrococcus furiosus and the bacterium Thermotoga petrophila are hyperthermophilic microorganisms that produce various enzymes with potential industrial, including β-glucosidases [19,25,26,36,41,42]. …”
Section: Discussionmentioning
confidence: 99%
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“…Briefly, endoglucanases randomly cleave the β-1,4-glycosidic linkages of cellulose, while cellobiohydrolases cleave off cellobiose units (dimer of glucose) from the reducing or nonreducing end of cellulose chain [4], [5]. At last, β-glucosidases hydrolyze the released cellobiose to glucose [6], [7].…”
Section: Introductionmentioning
confidence: 99%