Oligomerization of cytochrome c, myoglobin, and related heme proteins by 3D domain swapping

Hirota, Shun

doi:10.1016/j.jinorgbio.2019.03.002

Cited by 33 publications

(16 citation statements)

References 93 publications

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“…During purification the highly hydrophobic OmcS (Qian et al, 2011) and other proteins are separated from the lipid-rich environment of the outer-cell surface and suspended in high salt and high pH aqueous solutions. Changes in chemical conditions are known to induce c -type cytochrome monomers to polymerize into nanowires (Hirota et al, 2010; Haldar et al, 2015; Alvarez De Eulate et al, 2017; Hirota, 2019; Nucara et al, 2019). An important stabilizing element in the OmcS filament structure is the coordination of a histidine in one subunit with the iron in the heme of an adjacent subunit (Filman et al, 2019; Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Geobacter Protein Nanowires

2019

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The study of electrically conductive protein nanowires in Geobacter sulfurreducens has led to new concepts for long-range extracellular electron transport, as well as for the development of sustainable conductive materials and electronic devices with novel functions. Until recently, electrically conductive pili (e-pili), assembled from the PilA pilin monomer, were the only known Geobacter protein nanowires. However, filaments comprised of the multi-heme c-type cytochrome, OmcS, are present in some preparations of G. sulfurreducens outer-surface proteins. The purpose of this review is to evaluate the available evidence on the in vivo expression of e-pili and OmcS filaments and their biological function. Abundant literature demonstrates that G. sulfurreducens expresses e-pili, which are required for long-range electron transport to Fe (III) oxides and through conductive biofilms. In contrast, there is no definitive evidence yet that wild-type G. sulfurreducens express long filaments of OmcS extending from the cells, and deleting the gene for OmcS actually increases biofilm conductivity. The literature does not support the concern that many previous studies on e-pili were mistakenly studying OmcS filaments. For example, heterologous expression of the aromatic-rich pilin monomer of Geobacter metallireducens in G. sulfurreducens increases the conductivity of individual nanowires more than 5,000-fold, whereas expression of an aromatic-poor pilin reduced conductivity more than 1,000-fold. This more than million-fold range in nanowire conductivity was achieved while maintaining the 3-nm diameter characteristic of e-pili. Purification methods that eliminate all traces of OmcS yield highly conductive e-pili, as does heterologous expression of the e-pilin monomer in microbes that do not produce OmcS or any other outer-surface cytochromes. Future studies of G. sulfurreducens expression of protein nanowires need to be cognizant of the importance of maintaining environmentally relevant growth conditions because artificial laboratory culture conditions can rapidly select against e-pili expression. Principles derived from the study of e-pili have enabled identification of non-cytochrome protein nanowires in diverse bacteria and archaea. A similar search for cytochrome appendages is warranted. Both e-pili and OmcS filaments offer design options for the synthesis of protein-based “green” electronics, which may be the primary driving force for the study of these structures in the near future.

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Section: Introductionmentioning

confidence: 99%

Geobacter Protein Nanowires

2019

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“…Many c-type cyts can form oligomers by 3D domain swapping (herein, domain swapping) [3][4][5][6][7][8][9][10][11]. In domain swapping, two or more identical protein monomers exchange the same domain or secondary structural elements and fold into dimers or higher oligomers whose units are structurally similar to the original monomer [12][13][14][15]. Positively charged horse cytochrome (cyt) c forms domain-swapped oligomers by treatment with ethanol, and dimeric and trimeric horse cyt c are formed by swapping the Cterminal α-helix ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Protein surface charge effect on 3D domain swapping in cells for c-type cytochromes

Yang

Yamanaka

Nagao

et al. 2019

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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“…The first is contact dimerization, when the loop of one of the monomers forms stabilizing contacts with another molecule; the second is terminal domain exchange or domain swapping [ 51 ]. Domain exchange is typical of proteins such as cytochrome c [ 52 ] and, in particular, some amyloidogenic proteins, such as human prion protein, cystatin C, or β 2 -microglobulin [ 53 , 54 ].…”

Section: Secreted Ribonucleases Of Bacillimentioning

confidence: 99%

Supramolecular organization as a factor of ribonuclease cytotoxicity

2020

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One of the approaches used to eliminate tumor cells is directed destruction/modification of their RNA molecules. In this regard, ribonucleases (RNases) possess a therapeutic potential that remains largely unexplored. It is believed that the biological effects of secreted RNases, namely their antitumor and antiviral properties, derive from their catalytic activity. However, a number of recent studies have challenged the notion that the activity of RNases in the manifestation of selective cytotoxicity towards cancer cells is exclusively an enzymatic one. In this review, we have analyzed available data on the cytotoxic effects of secreted RNases, which are not associated with their catalytic activity, and we have provided evidence that the most important factor in the selective apoptosis-inducing action of RNases is the structural organization of these enzymes, which determines how they interact with cell components. The new idea on the preponderant role of non-catalytic interactions between RNases and cancer cells in the manifestation of selective cytotoxicity will contribute to the development of antitumor RNase-based drugs.

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Oligomerization of cytochrome c, myoglobin, and related heme proteins by 3D domain swapping

Cited by 33 publications

References 93 publications

Geobacter Protein Nanowires

Geobacter Protein Nanowires

Protein surface charge effect on 3D domain swapping in cells for c-type cytochromes

Supramolecular organization as a factor of ribonuclease cytotoxicity

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