Oligonucleotide composition of a yeast lysine transfer ribonucleic acid

Role of modified nucleosides in tRNA: Effect of modification of the 2-thiouridine derivative located at the 5'-end of the anticodon of yeast transfer RNA2Lys

“…So peak 17 must contain a decanucleotide of the composition (U3, C3, A, t6Al U*)G. Since both t6A and U* were present in the same oligonucleotide, peak 17 might be considered as the anticodon fragment of tRIN4YA . The composition of fragment 17 indeed agreed with thatof the T1 fragment containing the anticodon of tRNAys (7,21). tide obtained from chromatography of the T1 digest of (A) tRNAiYs and (B) tRNAiYs (CNBr).…”

5-Methyl-2-thiouridine in the tRNA of Candida tropicalis and its localization in lysine tRNA

“…Since both the thionucleotides were found in the RNase A-resistant fragment (see above), the results established that both of the thionucleotides were associated with Up only. The mobilities of the dinucleotides on DEAE-cellulose and Whatman 3 MM paper were in agreement for a dinucleotide of the type UJ*pUp or UpU*p. Observations similar to this have been made by other research workers, who encountered a RNase-resistant dinucleotide, mcm5s2Up followed by uri- (10,11,13,17). The present resu the presence of mcm5s2UpUp in the RNase tropicalis tRNA.…”