Oligonucleotide Detection and Optical Measurement with Graphene Oxide in the Presence of Bovine Serum Albumin Enabled by Use of Surfactants and Salts

Nitu, Florentin R.; Burns, Jorge S.; Ioniță, Mariana

doi:10.3390/coatings10040420

Cited by 7 publications

(6 citation statements)

References 61 publications

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“…ssDNA has a much higher binding efficiency on the GO surface than double-stranded (ds) DNA. There are numerous approaches where GO operates as a support nanomaterial for detecting DNA, including electrochemical [ 13 , 14 ], field-effect transistors [ 15 ], and fluorescent biosensor applications [ 16 , 17 ]. Among these, fluorescent base biosensors have been of particular interest due to their enhanced capabilities, such as low cost, simplicity, high sensitivity, superior selectivity, fast response, and multiple analyses [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Toader,

Nitu,

Ionita

2023

Molecules

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A nitrocellulose–graphene oxide hybrid that consists of a commercially nitrocellulose (NC) membrane non-covalently modified with graphene oxide (GO) microparticles was successfully prepared for oligonucleotide extraction. The modification of NC membrane was confirmed by Fourier Transform Infrared Spectroscopy (FTIR), which highlighted the principal absorption bands of both the NC membrane at 1641, 1276, and 835 cm−1 (NO2) and of GO in the range of 3450 cm−1 (CH2-OH). The SEM analysis underlined the well-dispersed and uniform coverage of NC membrane with GO, which displayed thin spider web morphology. The wettability assay indicated that the NC–GO hybrid membrane exhibited slightly lower hydrophilic behavior, with a water contact angle of 26.7°, compared to the 15° contact angle of the NC control membrane. The NC–GO hybrid membranes were used to separate oligonucleotides that had fewer than 50 nucleotides (nt) from complex solutions. The features of the NC–GO hybrid membranes were tested for extraction periods of 30, 45, and 60 min in three different complex solutions, i.e., an aqueous medium, an α-Minimum Essential Medium (αMEM), and an αMEM supplemented with fetal bovine serum (FBS). The oligonucleotides were desorbed from the surface of the NC–GO hybrid membrane using Tris-HCl buffer with a pH of 8.0. Out of the three media utilized, the best results were achieved after 60 min incubation of the NC–GO membranes in αMEM, as evidenced by the highest fluorescence emission of 294 relative fluorescence units (r.f.u.). This value corresponded to the extraction of approximately 330–370 pg (≈7%) of the total oligo-DNA. This method is an efficient and effortless way to purify short oligonucleotides from complex solutions.

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Section: Introductionmentioning

confidence: 99%

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Toader,

Nitu,

Ionita

2023

Molecules

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“…We chose to perform target-probe hybridization before adding GO to maximize hybridization opportunity. [25] The data indicates that GO is able to inhibit bioluminescence in this system in the absence of target, while maintaining signal in the presence of a target. The detection limit that we were able to obtain is 7.59 nM.…”

Section: Detection Of Hiv-1 Nucleic Acidmentioning

confidence: 81%

“…Unhybridized Gluc‐HIV‐1 probes would bind to the GO in this case as they are single stranded, thereby quenching the Gluc luminescence. We chose to perform target‐probe hybridization before adding GO to maximize hybridization opportunity [25] . The data indicates that GO is able to inhibit bioluminescence in this system in the absence of target, while maintaining signal in the presence of a target.…”

Section: Resultsmentioning

confidence: 99%

A Bioluminescent Protein‐Graphene Oxide Donor‐Quencher Pair in DNA Hybridization Assays

et al. 2022

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Despite fluorescent quenching with graphene oxide (GO) having shown great success in various applications -bioluminescent quenching has not yet been demonstrated using GO as a quencher. To explore the ability of GO to quench bioluminescence, we used Gaussia luciferase (Gluc) as a donor and GO as a quencher and demonstrated its application in sensing of two target analytes, HIV-1 DNA and IFN-γ. We demonstrated that the incubation of Gluc conjugated HIV-1 and IFN-γ oligonucleotide probes with GO provided for monitoring of probe-target interactions based on bioluminescence meas-urement in a solution phase sensing system. The limits of detection obtained for IFN-γ and HIV-1 DNA detection were 17 nM and 7.59 nM, respectively. Both sensing systems showed selectivity toward the target analyte. The detection of IFN-γ in saliva matrix was demonstrated. The use of GO as a quencher provides for high sensitivity while maintaining the selectivity of designed probes to their respective targets. The use of GO as a quencher provides for an easy assay design and low cost, environmentally friendly reporter.

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“…Fluorescence quenching efficiency is dependent on the interaction between DNA and GO, which is determined by the length of DNA [ 45 , 46 ], GO surface modification [ 25 ], size of GO [ 30 ], and concentrations of DNA and GO [ 47 ]. Compared to GO, the positively charged modified GO (pGO25000) had a perfect fluorescence quenching ability at very low concentrations.…”

Section: Resultsmentioning

confidence: 99%

Function of Graphene Oxide as the “Nanoquencher” for Hg2+ Detection Using an Exonuclease I-Assisted Biosensor

Sun

Jin

et al. 2022

IJMS

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Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg2+ detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg2+–T when Hg2+ is present, and no fluorescence is observed. It is digested by exonuclease I without Hg2+, and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg2+ concentration in the range of 0–250 nM (R2 = 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg2+ detection, obtaining a recovery rate from 99.6% to 101.1%.

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Oligonucleotide Detection and Optical Measurement with Graphene Oxide in the Presence of Bovine Serum Albumin Enabled by Use of Surfactants and Salts

Cited by 7 publications

References 61 publications

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

A Bioluminescent Protein‐Graphene Oxide Donor‐Quencher Pair in DNA Hybridization Assays

Function of Graphene Oxide as the “Nanoquencher” for Hg2+ Detection Using an Exonuclease I-Assisted Biosensor

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