Oligonucleotide Fingerprinting in Plants and Fungi

Weising, Kurt; Ramser, Juliane; Kaemmer, D.; Kahl, Günter; Epplen, Jörg T.

doi:10.1007/978-3-0348-7312-3_22

Cited by 247 publications

(267 citation statements)

References 65 publications

Supporting

Mentioning

246

Contrasting

Unclassified

Order By: Relevance

“…Alternatively, diagnostic marker bands of parents may have been lost from S. cambrensis due to rapid genomic change, which is believed to occur in new allopolyploids more commonly than previously assumed (Soltis & Soltis 1999, 2000. A third possibility is that band absence is due to the effect of ploidy upon competition processes in RAPD-PCR reactions (Wolff & Peters-Van Rijn 1993;Weising et al 1995). Because S. cambrensis contains a larger genome than S. squalidus, it is expected to possess more sites of 100% sequence similarity to a particular primer.…”

Section: Genomic Composition Of Hybrid Derivatives Of Senecio Squalidmentioning

confidence: 99%

Plant introductions, hybridization and gene flow

Abbott

James

Milne

et al. 2003

Phil. Trans. R. Soc. Lond. B

140

121

View full text Add to dashboard Cite

Many regional floras contain a high proportion of recently introduced plant species. Occasionally, hybridization between an introduced species and another species (introduced or native) can result in interspecific gene flow. This may occur even in instances where the F 1 hybrid shows very high sterility, but occasionally produces a few viable gametes. We provide examples of gene flow occurring between some rhododendrons recently introduced to the British flora, and between an introduced and native Senecio species. Neutral molecular markers have normally been employed to obtain evidence of interspecific gene flow, but the challenge now is to isolate and characterize functional introgressed genes and to determine how they affect the fitness of introgressants and whether they improve adaptation to novel habitats allowing introgressants to expand the range of a species. We outline a candidate gene approach for isolating and characterizing an allele of the RAY gene in Senecio vulgaris, which is believed to have introgressed from S. squalidus, and which causes the production of ray florets in flower heads. We discuss the effects of this introgressed allele on individual fitness, including those that originate directly from the production of ray florets plus those that may arise from pleiotropy and/or linkage.

show abstract

Section: Genomic Composition Of Hybrid Derivatives Of Senecio Squalidmentioning

confidence: 99%

Plant introductions, hybridization and gene flow

Abbott

James

Milne

et al. 2003

Phil. Trans. R. Soc. Lond. B

140

121

View full text Add to dashboard Cite

show abstract

“…Unfortunately, the traditional methods for characterization and assessment of genetic variability in perennial fruit crop species, based on morphological, physiological and biochemical studies are both time consuming and affected by the environment. The introduction of molecular biology techniques, such as DNA based markers, provides an opportunity for genetic characterization that allows direct comparison of different genetic material independent of environmental influences (Weising et al 1995). Previously, in addition to morphological identification (Zenelli et al 2005), various biochemical and molecular markers have been used for genetic characterization of walnut genotypes.…”

Section: Introductionmentioning

confidence: 99%

SSR and RAPD analysis of genetic diversity in walnut (Juglans regia L.) genotypes from Jammu and Kashmir, India

Ahmed

Mir

et al. 2012

Physiol Mol Biol Plants

View full text Add to dashboard Cite

“…Dados de microssatélites, obtidos para o gênero Musa (banana), mostram uma aplicação imediata para análise genética e sugerem que SSRs podem servir de âncora para a construção de um mapa genético básico em banana (Kaemmer et al, 1997). Marcadores SSRs podem também ser obtidos, usando oligonucleotídeos de seqüências simples repetidas como primers na amplificação de DNA por PCR ou, ainda, como sondas em experimentos de hibridação envolvendo DNA genômico, para a detecção de polimorfismo em famílias de DNA repetitivo (Weising et al, 1995). Esta abordagem foi usada para a análise genética em Helianthus (Dehmer, 1998) e Solanum tuberosum (McGregor et al, 2000).…”

Section: Introductionunclassified

Uso de marcadores moleculares na análise da variabilidade genética em acerola (Malpighia emarginata D.C.)

Salla

Ruas

et al. 2002

Rev. Bras. Frutic.

View full text Add to dashboard Cite

RESUMO -A acerola (Malpighia emarginata) é uma frutífera tropical encontrada nativa na América Central e no Norte da América do Sul, sendo de grande importância econômica e social devido ao seu alto conteúdo de vitamina C (ácido ascórbico). Pomares de acerola têm sido preferencialmente estabelecidos por métodos de propagação vegetiva. No entanto, a propagação sexuada por sementes é igualmente utilizada e permite revelar um alto grau de polimorfismo na cultura, possibilitando a identificação de genótipos portadores de características de interesse agronômico. Vinte e quatro acessos de acerola, pertencentes ao Banco Ativo de Germoplasma da Universidade Estadual de Londrina, foram analisados, usando marcadores RAPD (Random amplified Polymorphic DNA) e obtidos com iniciadores (primers) de seqüência simples repetidas (SSRs). Um total de 164 e 73 marcadores foram obtidos com primers de RAPD e SSR, respectivamente. Os marcadores obtidos foram analisados, usando o método de agrupamentos UPGMA. A análise comparativa dos dendrogramas gerados com os primers de RAPD e com os primers SSR mostrou que, enquanto alguns acessos se associaram em grupos diferentes, outros apresentaram a mesma associação. Entretanto, maior polimorfismo entre acessos foi detectado com os primers de RAPD. A análise dos resultados revelou a alta variabilidade contida na coleção, permitindo associar o grau de similaridade genética, obtido por marcadores de DNA, com caracteres morfológicos compartilhados entre os acessos.Termos para indexação: Acerola, Malpighia emarginata, marcadores RAPD e SSR, variabilidade genética. THE USE OF MOLECULAR MARKERS IN THE GENETIC VARIABILITY ANALYSIS OF ACEROLA (Malphighia emarginata)ABSTRACT -Acerola (Malpighia emarginata) is a tropical fruit native from Central America and north of South America. It has shown an increasing economic and social importance due to its high vitamin C (ascorbic acid) content. Vegetative propagation is the preferable method used to establish acerola plantations. However, propagation by seeds has also been used allowing the identification and selection of genotypes that carry characteristics of agronomic interest. Twenty-four acerola accesses, of the Active Germplasm Bank of the Universidade Estadual de Londrina, were analyzed using molecular markers obtained with random amplified polymorphic DNA (RAPD) and simple sequence repeated (SSRs) primers. A total of 164 and 73 markers were obtained with RAPD and SSR primers, respectively. Polymorfic markers were scored as present or absent and analyzed using the UPGMA cluster analysis. The results presented reveal high levels of polymorphism in the studied collection. Comparative analysis of the phenograms, generated with both RAPD and SSR primers, revealed that while some accesses clustered in different groups other accesses presented the same association. However, there was large RAPD variation among the accesses. The associations observed with molecular markers were, for many accesses, the same of those determined on the basis of morphological char...

show abstract

Oligonucleotide Fingerprinting in Plants and Fungi

Cited by 247 publications

References 65 publications

Plant introductions, hybridization and gene flow

Plant introductions, hybridization and gene flow

SSR and RAPD analysis of genetic diversity in walnut (Juglans regia L.) genotypes from Jammu and Kashmir, India

Uso de marcadores moleculares na análise da variabilidade genética em acerola (Malpighia emarginata D.C.)

Contact Info

Product

Resources

About