Oligonucleotide fingerprints of antigenically related bovine coronavirus and human coronavirus OC43

Lapps, William; Brian, David A.

doi:10.1007/bf01314116

Cited by 18 publications

(16 citation statements)

References 21 publications

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“…We showed previously that BCV probes specific to different regions throughout the genome were individually capable of detecting TCV isolates or TCV in clinical specimens (unpublished results). Since homology between BCV and HCV-OC43 has been confirmed by serological studies (Hogue et al, 1984), and RNA fingerprinting data suggest a close resemblance between the remaining as yet unsequenced portions of the genomes (Lapps & Brian, 1985), we also expect an overall genomic relationship between TCV and HCV-OC43, although this has to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus

Verbeek¹,

Tijssen²

1991

Journal of General Virology

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The 3' end of the turkey coronavirus (TCV) genome and the gene encoding the nucleocapsid protein (N) were cloned and sequenced. The gene encoding the membrane protein (M) was obtained by cloning a polymerase chain reaction (PCR)-amplified fragment obtained using bovine coronavirus (BCV)-specific primers. Furthermore, five TCV DNA fragments, obtained by PCR on RNA from clinical specimens and corresponding to either the N terminus of the M protein or the complete M protein were also cloned and sequenced. The sequence revealed a 3' non-coding region of 291 bases, an open reading frame (ORF) encoding the N protein with a predicted size of 448 amino acids, or an Mr of 49K, and an ORF encoding the M protein with a predicted size of 230 amino acids and an Mr of 26K. A third ORF, encoding a hypothetical protein of 207 amino acids with an Mr of 23K was found within the N gene sequence. The amino acid sequences of both the N and M proteins were more than 99% similar to those published for BCV. Extensive similarity was also observed between the amino acid sequences of the TCV N protein and those of murine hepatitis virus (MHV) (70%) and human respiratory coronavirus strain OC43 (HCV-OC43) (98%) and between the amino acid sequences of the predicted M proteins of TCV and MHV (86 %). Such striking identity suggests that BCV, TCV and HCV-OC43 must have diverged from each other only recently. A potential N-glycosylation site was found at the N terminus of the TCV M protein and is situated at the same location in BCV, MHV and transmissible gastroenteritis virus.

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Section: Discussionmentioning

confidence: 99%

Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus

Verbeek¹,

Tijssen²

1991

Journal of General Virology

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“…The Mebus strain of bovine coronavirus (BCV) was plaque purified and grown on the human rectal tumor (HRT) cell line as previously described (Hogue et al, 1984;Lapps and Brian, 1985).…”

Section: Virus and Cellsmentioning

confidence: 99%

“…Viral glycoproteins were labeled by adding 400 &i of [3H]glucosamine (5-l 5 Ci/mmol, ICN) per flask to me-dium containing 5% fetal calf serum. Virus was harvested and purified by isopycnic sedimentation in continuous sucrose gradients as previously described (Hogue et a/., 1984;Lapps and Brian, 1985). g, 25".…”

Section: Radiolabeling Of Viral Proteins and Purification Of Virusmentioning

confidence: 99%

“…BCV is known to possess a single-stranded, nonsegmented, polyadenylated RNA genome of approximately 20 kb (Guy and Brian, 1979;Lapps and Brian, 1985). The total number of genes encoded by the genome is not known, but presumably, because of its close antigenic relatedness to the well-characterized mouse hepatitis coronavirus, BCV will be similar to MHV in the number and arrangement of genes on its genome.…”

Section: Introductionmentioning

confidence: 99%

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Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes

1987

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The 3' end of the 20-kb genome of the Mebus strain of bovine enteric coronavirus (BCV) was copied into cDNA and cloned into the PstI site of the pUC9 vector. Four clones from the 3' end of the genome were sequenced either completely or in part to determine the sequence of the first 2451 bases. Within this sequence were identified, in order, a 3'-noncoding region of 291 bases, the gene for a 448-amino acid nucleocapsid protein (N) having a molecular weight of 49,379, and the gene for a 230-amino acid matrix protein (M) having a molecular weight of 26,376. A third large open reading frame is contained entirely within the N gene sequence but is positioned in a different reading frame; it potentially encodes a polypeptide of 207 amino acids having a molecular weight of 23,057. A higher degree of amino acid sequence homology was found between the M proteins of BCV and MHV (87%) than between the N proteins (70%). For the M proteins of BCV and MHV, notable differences were found at the amino terminus, the most probable site of O-glycosylation, where the sequence is N-Met-Ser-Ser-Val-Thr-Thr for BCV and N-Met-Ser-Ser-Thr-Thr for MHV. BCV apparently uses two of its six potential O-glycosylation sites.

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“…HCoV-OC43 causes the common cold in humans [1]. BCoV and HCoV-OC43 have marked antigenic and genetic homologies, and the emergence of HCoV-OC43 after cattle-to-human transmission of BCoV has also been reported [9,[15][16][17]. A gene sequence similarity suggests that CRCoV was also derived from BCoV or HCoV-OC43; however, since the entire gene of CRCoV has not been sequenced, its details remain unknown.…”

mentioning

confidence: 99%

The Infectivity and Pathogenicity of a Group 2 Bovine Coronavirus in Pups

Kaneshima

Hohdatsu

Hagino

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. Canine respiratory coronavirus (CRCoV), which is more closely related to the bovine coronavirus (BCoV), has recently been detected in dogs. In this study, we examined whether BCoV was capable of infecting and exhibiting pathogenicity in dogs. Three 1-month-old pups were oronasally given field isolates of BCoV, and were kept together with 2 control animals. As a result, increases in BCoV-neutralizing antibody titers were confirmed in all pups in the challenged and control groups. Moreover, the virus gene was also detected in oral and rectal swabs by RT-PCR. These results indicate that BCoV infects dogs, and easily infects other dogs that are kept together. However, no clinical symptoms such as respiratory symptoms and diarrhea were observed.

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Oligonucleotide fingerprints of antigenically related bovine coronavirus and human coronavirus OC43

Cited by 18 publications

References 21 publications

Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus

Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus

Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes

The Infectivity and Pathogenicity of a Group 2 Bovine Coronavirus in Pups

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